2013
Human embryonic poly(A)-binding protein (EPAB) alternative splicing is differentially regulated in human oocytes and embryos
Guzeloglu-Kayisli O, Lalioti MD, Babayev E, Torrealday S, Karakaya C, Seli E. Human embryonic poly(A)-binding protein (EPAB) alternative splicing is differentially regulated in human oocytes and embryos. Molecular Human Reproduction 2013, 20: 59-65. PMID: 24002949, DOI: 10.1093/molehr/gat061.Peer-Reviewed Original ResearchConceptsZygotic genome activationEarly embryo developmentEarly embryosTranslational activationSomatic tissuesSpliced formsEmbryo developmentEmbryo-specific expressionPost-transcriptional mechanismsOocyte maturationHuman somatic tissuesFull-length formGenome activationExon 8Transcriptional regulationAlternative splicingTranscriptional activityGene expressionHuman oocytesEPABAmino acidsXenopusEmbryosMRNAOocytesCharacterization of the Gonadotropin Releasing Hormone Receptor (GnRHR) Expression and Activity in the Female Mouse Ovary
Torrealday S, Lalioti MD, Guzeloglu-Kayisli O, Seli E. Characterization of the Gonadotropin Releasing Hormone Receptor (GnRHR) Expression and Activity in the Female Mouse Ovary. Endocrinology 2013, 154: 3877-3887. PMID: 23913446, PMCID: PMC3776864, DOI: 10.1210/en.2013-1341.Peer-Reviewed Original ResearchConceptsGranulosa/cumulus cellsGranulosa cellsMouse ovariesReceptor expressionPrimary mouse granulosa cellsCumulus cellsPregnant mare serum gonadotropinIntracellular cAMPHormone receptor expressionGnRH receptor expressionCultured granulosa cellsOvarian granulosa cellsMouse granulosa cellsMechanism of actionGonadotoxic chemotherapyQuantitative RT-PCRGnRH agonistGnRHR mRNAFertility preservationMouse pituitary cellsLuciferase reporter plasmidSerum gonadotropinPituitary cell linePituitary cellsPituitary tissuemRNA-Binding Protein ZFP36 Is Expressed in Atherosclerotic Lesions and Reduces Inflammation in Aortic Endothelial Cells
Zhang H, Taylor WR, Joseph G, Caracciolo V, Gonzales DM, Sidell N, Seli E, Blackshear PJ, Kallen CB. mRNA-Binding Protein ZFP36 Is Expressed in Atherosclerotic Lesions and Reduces Inflammation in Aortic Endothelial Cells. Arteriosclerosis Thrombosis And Vascular Biology 2013, 33: 1212-1220. PMID: 23559629, PMCID: PMC3844532, DOI: 10.1161/atvbaha.113.301496.Peer-Reviewed Original ResearchConceptsVascular endothelial cellsEndothelial cellsAortic endothelial cellsAtherosclerotic lesionsFoam cellsAnti-inflammatory effectsHuman aortic endothelial cellsNuclear factor-κB transcriptional activationLow-density lipoproteinMacrophage foam cellsZinc finger protein 36Vascular inflammationAngiotensin IIInflammatory cytokinesCytokine mRNAZFP36 expressionBacterial lipopolysaccharideHealthy aortaInflammationTarget cellsLesionsZFP36Protein 36CellsMRNA transcripts
2012
Embryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation
Friend K, Brook M, Bezirci FB, Sheets MD, Gray NK, Seli E. Embryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation. Biochemical Journal 2012, 445: 93-100. PMID: 22497250, PMCID: PMC3955212, DOI: 10.1042/bj20120304.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCytoplasmFemaleFluorescent Antibody TechniqueGene Expression Regulation, DevelopmentalImmunoprecipitationMutationOocytesOogenesisPhosphorylationPoly APoly(A)-Binding ProteinsPolyadenylationProtein BiosynthesisProtein Processing, Post-TranslationalRNA, MessengerXenopus laevisXenopus ProteinsConceptsCytoplasmic polyadenylationOocyte maturationPost-transcriptional regulatorsEarly embryonic developmentPost-translational modificationsXenopus oocyte maturationMaternal mRNAsProtein phosphorylationResidue clustersTranslational activationEmbryonic developmentXenopus laevis oocytesProtein activityPhosphorylationFirst insightLaevis oocytesPolyadenylationInherent abilityEPABMaturationPhosphoproteinRegulatorMutationsMRNAOocytesThe mRNA‐Binding Protein Zfp36 Is Upregulated by β‐Adrenergic Stimulation and Represses IL‐6 Production in 3T3‐L1 Adipocytes
Brahma PK, Zhang H, Murray BS, Shu F, Sidell N, Seli E, Kallen CB. The mRNA‐Binding Protein Zfp36 Is Upregulated by β‐Adrenergic Stimulation and Represses IL‐6 Production in 3T3‐L1 Adipocytes. Obesity 2012, 20: 40-47. PMID: 21818148, PMCID: PMC4127993, DOI: 10.1038/oby.2011.259.Peer-Reviewed Original ResearchConceptsAU-rich elementsZFP36 expressionCell type-specific modulatorsPost-transcriptional levelMRNA-binding proteinRetinoic acidZinc finger protein 36Novel mechanistic approachTarget mRNAsMRNA targetsSuch proteinsPotent repressorAdipocyte-derived IL-6Protein 36Chronic inflammatory stateSystemic immune responsesDevelopment of diabetesDependent pathwayDiverse inflammatory disordersZFP36IL-6 productionAdrenergic receptor agonist isoproterenolCyclic adenosine monophosphateInterleukin-6 expressionΒ-Adrenergic Stimulation
2011
The mRNA-Binding Protein HuR is Regulated in the Menstrual Cycle and Repressed in Ectopic Endometrium
Karipcin FS, Ensari TA, Kayisli UA, Guzel E, Kallen CB, Seli E. The mRNA-Binding Protein HuR is Regulated in the Menstrual Cycle and Repressed in Ectopic Endometrium. Reproductive Sciences 2011, 18: 145-155. PMID: 20889954, PMCID: PMC3343139, DOI: 10.1177/1933719110382307.Peer-Reviewed Original ResearchConceptsEctopic endometriumMenstrual cycleSecretory phaseCytokine expressionHuR expressionNormal endometriumCultured endometrial stromal cellsStromal cellsEctopic endometrial cellsEctopic human endometriumMid-menstrual phaseElevated cytokine levelsEndometrial stromal cellsLate secretory phasePathogenesis of endometriosisCultured stromal cellsTumor necrosis factorMessenger RNACytokine levelsEndometrial cellsEutopic endometriumHuman endometriumNecrosis factorEndometriumEndometriosis
2010
Identification and in Vitro Characterization of Follicle Stimulating Hormone (FSH) Receptor Variants Associated with Abnormal Ovarian Response to FSH
Gerasimova T, Thanasoula MN, Zattas D, Seli E, Sakkas D, Lalioti MD. Identification and in Vitro Characterization of Follicle Stimulating Hormone (FSH) Receptor Variants Associated with Abnormal Ovarian Response to FSH. The Journal Of Clinical Endocrinology & Metabolism 2010, 95: 529-536. PMID: 20061434, PMCID: PMC2840851, DOI: 10.1210/jc.2009-1304.Peer-Reviewed Original ResearchConceptsOvarian responseFSH receptorFSHR mRNAAbnormal ovarian responseOvarian stimulation protocolsForms of infertilityOocytes of womenExtracellular ligand-binding portionHormone receptor variantsOvarian hyperstimulationOvarian stimulationFSHR variantsPoor responseInfertility treatmentT cellsStimulation protocolFollicle growthFSHFertility clinicsCAMP activationCumulus cellsReceptor variantsGenetic causeRT-PCRWomen
2008
Alternative splicing of the mouse embryonic poly(A) binding protein (Epab) mRNA is regulated by an exonic splicing enhancer: a model for post-transcriptional control of gene expression in the oocyte
Seli E, Yaba A, Guzeloglu-Kayisli O, Lalioti MD. Alternative splicing of the mouse embryonic poly(A) binding protein (Epab) mRNA is regulated by an exonic splicing enhancer: a model for post-transcriptional control of gene expression in the oocyte. Molecular Human Reproduction 2008, 14: 393-398. PMID: 18492745, PMCID: PMC2453241, DOI: 10.1093/molehr/gan028.Peer-Reviewed Original ResearchConceptsExonic splicing enhancersAlternative splicing variantsSingle nucleotide polymorphismsAlternative splicingSplicing variantsSplicing enhancersExon 10Post-transcriptional controlMRNA alternative splicingEarly preimplantation embryosOocyte-specific proteinRNA editingMaternal mRNAsEarly embryosMouse embryonicBinding protein mRNAReal-time RT-PCR resultsGene expressionPreimplantation embryosSpliced formsSame locusSequence analysisBinding proteinSplicingParental origin
2005
An embryonic poly(A)-binding protein (ePAB) is expressed in mouse oocytes and early preimplantation embryos
Seli E, Lalioti MD, Flaherty SM, Sakkas D, Terzi N, Steitz JA. An embryonic poly(A)-binding protein (ePAB) is expressed in mouse oocytes and early preimplantation embryos. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 367-372. PMID: 15630085, PMCID: PMC544294, DOI: 10.1073/pnas.0408378102.Peer-Reviewed Original ResearchConceptsZygotic gene activationGene activationEarly embryosSomatic cellsTranslational activationGene expressionEmbryo developmentEarly preimplantation embryo developmentEarly Xenopus developmentEarly preimplantation embryosEight-cell stageEarly embryo developmentPreimplantation embryo developmentTwo-cell embryosCytoplasmic PABPMouse orthologXenopus developmentMammalian oocytesProphase ISomatic tissuesChromosome 2Preimplantation embryosEPABMouse oocytesOne-cell
2002
Estradiol down-regulates MCP-1 expression in human coronary artery endothelial cells
Seli E, Pehlivan T, Selam B, Garcia-Velasco JA, Arici A. Estradiol down-regulates MCP-1 expression in human coronary artery endothelial cells. Fertility And Sterility 2002, 77: 542-547. PMID: 11872210, DOI: 10.1016/s0015-0282(01)03223-x.Peer-Reviewed Original ResearchConceptsHuman coronary artery endothelial cellsCoronary artery endothelial cellsMCP-1 expressionHuman umbilical vein endothelial cellsEnzyme-linked immunosorbent assayArtery endothelial cellsMCP-1 mRNAUmbilical vein endothelial cellsEndothelial cellsVein endothelial cellsMCP-1Prospective comparative studyConcentrations of estradiolMCP-1 productionMCP-1 proteinDose-dependent inhibitionVascular endothelial cellsEstrogen treatmentMacrophage recruitmentMAIN OUTCOMEMRNA expressionRibonucleic acidImmunosorbent assayProtein expressionTamoxifen
1999
Monocyte chemotactic protein-1 expression in human corpus luteum
Senturk LM, Seli E, Gutierrez LS, Mor G, Zeyneloglu HB, Arici A. Monocyte chemotactic protein-1 expression in human corpus luteum. Molecular Human Reproduction 1999, 5: 697. PMID: 10421794, DOI: 10.1093/molehr/5.8.697.Peer-Reviewed Original Research
1998
The role of monocyte chemotactic protein-1 in intraperitoneal adhesion formation.
Zeyneloglu HB, Senturk LM, Seli E, Oral E, Olive DL, Arici A. The role of monocyte chemotactic protein-1 in intraperitoneal adhesion formation. Human Reproduction 1998, 13: 1194-1199. PMID: 9647546, DOI: 10.1093/humrep/13.5.1194.Peer-Reviewed Original ResearchConceptsMCP-1 levelsChemotactic protein-1Enzyme-linked immunosorbent assayIntraperitoneal adhesion formationIntraperitoneal adhesionsPg/Monocyte chemotactic protein-1MCP-1 mRNA expressionNormal fertile womenBilateral tubal ligationProtein 1MCP-1 proteinAdhesion formationGroup of womenWound repair modelPelvic adhesionsFertile womenMononuclear cellsPeritoneal injuryMCP-1Tubal ligationReproductive ageEndometriosisBiopsy samplesPatients
1997
Leukaemia inhibitory factor expression in human follicular fluid and ovarian cells.
Arici A, Oral E, Bahtiyar O, Engin O, Seli E, Jones EE. Leukaemia inhibitory factor expression in human follicular fluid and ovarian cells. Human Reproduction 1997, 12: 1233-1239. PMID: 9222008, DOI: 10.1093/humrep/12.6.1233.Peer-Reviewed Original ResearchConceptsFollicular fluid samplesLeukemia inhibitory factorLIF mRNAOvarian stromal cellsGranulosa-lutein cellsFollicular phaseFollicular fluidFluid samplesExpression of LIFPg/Embryo qualityStromal cellsFollicular fluid oestradiol concentrationsLeukemia inhibitory factor expressionTumor necrosis factor alphaPhysiology of ovulationNecrosis factor alphaConcentrations of LIFHuman follicular fluidConcentration-dependent mannerLIF levelsOvarian stimulationStromal cell culturesOvarian functionOestradiol concentrations
1996
Growth-regulated alpha expression in the peritoneal environment with endometriosis.
Oral E, Seli E, Bahtiyar MO, Olive DL, Arici A. Growth-regulated alpha expression in the peritoneal environment with endometriosis. Obstetrics And Gynecology 1996, 88: 1050-6. PMID: 8942852, DOI: 10.1016/s0029-7844(96)00361-4.Peer-Reviewed Original ResearchConceptsAlpha messenger RNAPeritoneal fluid samplesPeritoneal fluidPg/Severe endometriosisFertile womenSevere endometriosis groupNormal fertile womenNeutrophil chemotactic activityReproductive-age womenEndometrial stromal cellsPathogenesis of endometriosisFluid samplesInterleukin-1 alphaMessenger RNAInterleukin-8 receptorEnzyme-linked immunosorbentMann-Whitney testUntreated endometriosisEndometrial biopsyTime-dependent mannerEndometrial diseaseEndometriosis groupEndometrial tissuePeritoneal environment