2020
Impaired Mitochondrial Stress Response due to CLPP Deletion Is Associated with Altered Mitochondrial Dynamics and Increased Apoptosis in Cumulus Cells
Esencan E, Jiang Z, Wang T, Zhang M, Soylemez-Imamoglu G, Seli E. Impaired Mitochondrial Stress Response due to CLPP Deletion Is Associated with Altered Mitochondrial Dynamics and Increased Apoptosis in Cumulus Cells. Reproductive Sciences 2020, 27: 621-630. PMID: 31939198, DOI: 10.1007/s43032-019-00063-y.Peer-Reviewed Original ResearchConceptsCaseinolytic peptidase PCumulus cell functionClpP deletionMitochondrial unfolded protein responseMitochondrial stress responseCumulus cellsUnfolded protein responseRNA sequencing analysisAltered mitochondrial dynamicsCell functionProtein homeostasisMitochondrial dynamics genesCLPP resultsMitochondrial dynamicsDynamic genesPhagosome pathwayProtein responseCellular metabolismGene expressionWild typeStress responseCumulus oophorus complexesMitochondrial ultrastructureSequencing analysisApoptotic activity
2019
Mitofusin 1 is required for female fertility and to maintain ovarian follicular reserve
Zhang M, Bener MB, Jiang Z, Wang T, Esencan E, Scott III R, Horvath T, Seli E. Mitofusin 1 is required for female fertility and to maintain ovarian follicular reserve. Cell Death & Disease 2019, 10: 560. PMID: 31332167, PMCID: PMC6646343, DOI: 10.1038/s41419-019-1799-3.Peer-Reviewed Original ResearchConceptsOocyte-granulosa cell communicationDynamic organellesAccumulation of ceramideFemale reproductive agingMitofusin 1Secondary follicle stageMitochondrial dynamicsCell communicationReproductive phenotypesCeramide synthesis inhibitor myriocinDevelopmental arrestApoptotic cell lossMitochondrial dysfunctionTargeted deletionOvarian follicular reserveOocyte maturationFemale fertilityFollicle stageDeletionPhenotypeReproductive agingOocytesCadherinFollicular reserveOrganellesMitofusin 2 plays a role in oocyte and follicle development, and is required to maintain ovarian follicular reserve during reproductive aging
Zhang M, Bener MB, Jiang Z, Wang T, Esencan E, Scott R, Horvath T, Seli E. Mitofusin 2 plays a role in oocyte and follicle development, and is required to maintain ovarian follicular reserve during reproductive aging. Aging 2019, 11: 3919-3938. PMID: 31204316, PMCID: PMC6628992, DOI: 10.18632/aging.102024.Peer-Reviewed Original ResearchConceptsMitofusin 2Key regulatory proteinsImpaired oocyte maturationFollicle developmentMitochondrial fusionRegulatory proteinsEndoplasmic reticulumMitochondrial dysfunctionTargeted deletionOocyte maturationOocytesReproductive agingFemale subfertilityOocyte qualityOvarian follicular reserveTelomeresMitochondriaMetabolic milieuProteinReticulumDeletionFusionPhenotypeApoptosisMaturation
2018
Metabolic imaging with the use of fluorescence lifetime imaging microscopy (FLIM) accurately detects mitochondrial dysfunction in mouse oocytes
Sanchez T, Wang T, Pedro MV, Zhang M, Esencan E, Sakkas D, Needleman D, Seli E. Metabolic imaging with the use of fluorescence lifetime imaging microscopy (FLIM) accurately detects mitochondrial dysfunction in mouse oocytes. Fertility And Sterility 2018, 110: 1387-1397. PMID: 30446247, PMCID: PMC6289735, DOI: 10.1016/j.fertnstert.2018.07.022.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedComputer SystemsEmbryo Culture TechniquesEmbryo, MammalianEmbryonic DevelopmentEndopeptidase ClpFemaleFlavin-Adenine DinucleotideFluorescenceMaleMaternal AgeMiceMice, Inbred C57BLMice, KnockoutMicroscopy, FluorescenceMitochondriaMolecular ImagingNADOocytesReactive Oxygen SpeciesConceptsBlastocyst development rateOocyte dysfunctionReactive oxygen species levelsFlavin adenine dinucleotide (FAD) autofluorescenceMetabolic dysfunctionOxygen species levelsYoung miceMetabolic parametersOld miceMAIN OUTCOMEGlobal knockoutDysfunctionNoninvasive toolNormal oocytesMetabolic imagingMitochondrial dysfunctionMiceOld oocytesFLIM parametersROS levelsMetabolic differencesMitochondrial functionNicotinamide adenine dinucleotide dehydrogenaseIndividual oocytesWild-type oocytesMitochondrial unfolded protein response gene Clpp is required to maintain ovarian follicular reserve during aging, for oocyte competence, and development of pre‐implantation embryos
Wang T, Babayev E, Jiang Z, Li G, Zhang M, Esencan E, Horvath T, Seli E. Mitochondrial unfolded protein response gene Clpp is required to maintain ovarian follicular reserve during aging, for oocyte competence, and development of pre‐implantation embryos. Aging Cell 2018, 17: e12784. PMID: 29851234, PMCID: PMC6052477, DOI: 10.1111/acel.12784.Peer-Reviewed Original ResearchConceptsMitochondrial unfolded protein responseUnfolded mitochondrial proteinsCaseinolytic peptidase PAbsence of ClpPUnfolded protein responsePre-implantation embryosExpression of genesOocyte mitochondrial functionTwo-cell embryosProtein homeostasisMTOR inhibitor rapamycinMitochondrial proteinsOocyte competenceClpPProtein responseInhibitor rapamycinMitochondrial functionP-Akt473P-S6KOvarian follicular reserveSmall mitochondriaMTOR pathway activationPathway activationEmbryosP-S6
2017
Embryonic poly(A)-binding protein is required at the preantral stage of mouse folliculogenesis for oocyte–somatic communication†
Lowther KM, Favero F, Yang CR, Taylor HS, Seli E. Embryonic poly(A)-binding protein is required at the preantral stage of mouse folliculogenesis for oocyte–somatic communication†. Biology Of Reproduction 2017, 96: 341-351. PMID: 28203794, DOI: 10.1095/biolreprod.116.141234.Peer-Reviewed Original Research
2015
Embryonic Poly(A)-Binding Protein (EPAB) Is Required for Granulosa Cell EGF Signaling and Cumulus Expansion in Female Mice
Yang CR, Lowther KM, Lalioti MD, Seli E. Embryonic Poly(A)-Binding Protein (EPAB) Is Required for Granulosa Cell EGF Signaling and Cumulus Expansion in Female Mice. Endocrinology 2015, 157: 405-416. PMID: 26492470, PMCID: PMC4701890, DOI: 10.1210/en.2015-1135.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Morphogenetic Protein 15Cell ProliferationCells, CulturedCoculture TechniquesCumulus CellsEpidermal Growth FactorErbB ReceptorsFemaleGranulosa CellsGrowth Differentiation Factor 9Luteinizing HormoneMAP Kinase Signaling SystemMice, KnockoutOocytesPhosphorylationPoly(A)-Binding ProteinsProtein Processing, Post-TranslationalReceptors, LHSignal TransductionConceptsEpidermal growth factorZygotic genome activationP90 ribosomal S6 kinaseBone morphogenetic protein 15Cumulus cellsRibosomal S6 kinaseImpaired oocyte maturationCumulus expansionGrowth differentiation factor 9Genome activationDifferentiation factor 9S6 kinaseEarly embryosTranslational activationEGF signalingEGF receptorFemale micePhosphorylated MEK1/2EGF treatmentBinding proteinFactor 9Cells exhibitOocyte maturationProteinProtein 15
2014
Epab is dispensable for mouse spermatogenesis and male fertility
Ozturk S, Guzeloglu-Kayisli O, Lowther KM, Lalioti MD, Sakkas D, Seli E. Epab is dispensable for mouse spermatogenesis and male fertility. Molecular Reproduction And Development 2014, 81: 390-390. PMID: 24599567, PMCID: PMC4079073, DOI: 10.1002/mrd.22319.Peer-Reviewed Original Research
2012
Embryonic poly(A)-binding protein (EPAB) is required for oocyte maturation and female fertility in mice
Guzeloglu-Kayisli O, Lalioti MD, Aydiner F, Sasson I, Ilbay O, Sakkas D, Lowther KM, Mehlmann LM, Seli E. Embryonic poly(A)-binding protein (EPAB) is required for oocyte maturation and female fertility in mice. Biochemical Journal 2012, 446: 47-58. PMID: 22621333, PMCID: PMC3955213, DOI: 10.1042/bj20120467.Peer-Reviewed Original ResearchConceptsTranslational activationOocyte maturationZygotic genome activationFemale fertilityGenome activationGerminal vesicle stage oocytesDazl mRNAEarly embryogenesisMaternal mRNAsDownstream regulatorsMammalian reproductionGene expressionEPABStage oocytesXenopus oocytesEpidermal growthLate antral folliclesOogenesisMRNAOocytesMaturationProteinFemale miceCumulus expansionAntral follicles
2007
Estrogen increases apoptosis in the arterial wall in a murine atherosclerosis model
Seli E, Guzeloglu-Kayisli O, Kayisli UA, Kizilay G, Arici A. Estrogen increases apoptosis in the arterial wall in a murine atherosclerosis model. Fertility And Sterility 2007, 88: 1190-1196. PMID: 17498707, DOI: 10.1016/j.fertnstert.2007.01.132.Peer-Reviewed Original ResearchConceptsHigh-cholesterol dietMurine atherosclerosis modelAtherosclerotic plaque formationCholesterol dietFemale LDLAtherosclerosis modelPlaque formationArterial wallPlacebo-treated miceMitotic activityEffects of estrogenEffects of ovariectomyAtherosclerotic plaque developmentVascular effectsFemale miceMAIN OUTCOMEVascular apoptosisC57BL/6 backgroundPlaque developmentAortic wallLDLLDL receptorMice resultsMiceDNA strand breaks
2002
Estradiol suppresses vascular monocyte chemotactic protein-1 expression during early atherogenesis
Seli E, Kayisli UA, Selam B, Seli M, Arici A. Estradiol suppresses vascular monocyte chemotactic protein-1 expression during early atherogenesis. American Journal Of Obstetrics And Gynecology 2002, 187: 1544-1549. PMID: 12501061, DOI: 10.1067/mob.2002.127306.Peer-Reviewed Original ResearchConceptsMonocyte chemotactic protein-1 expressionChemotactic protein-1 expressionProtein-1 expressionLipid depositionSerum total cholesterol concentrationVascular lipid depositionProtein 1 antibodyAorta of animalsTotal cholesterol concentrationDevelopment of atherosclerosisSmooth muscle cellsPlacebo pelletsAtherogenic stimuliDeficient miceMacrophage recruitmentEarly atherogenesisImmunohistochemical analysisTreatment groupsC57BL/6 backgroundCholesterol concentrationsMuscle cellsArterial wallB stainingAortaEstradiol