2021
Human embryo polarization requires PLC signaling to mediate trophectoderm specification
Zhu M, Shahbazi M, Martin A, Zhang C, Sozen B, Borsos M, Mandelbaum RS, Paulson RJ, Mole MA, Esbert M, Titus S, Scott RT, Campbell A, Fishel S, Gradinaru V, Zhao H, Wu K, Chen ZJ, Seli E, de los Santos MJ, Goetz M. Human embryo polarization requires PLC signaling to mediate trophectoderm specification. ELife 2021, 10: e65068. PMID: 34569938, PMCID: PMC8514238, DOI: 10.7554/elife.65068.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdultBody PatterningCell DifferentiationCell LineageCell PolarityEmbryo Culture TechniquesEmbryo, MammalianFemaleGATA3 Transcription FactorGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicHumansPhosphoinositide Phospholipase CPhospholipase C betaPregnancySignal TransductionTime FactorsYoung AdultConceptsApical domain formationInner cell massEmbryo polarizationPhospholipase CFirst lineage segregationApico-basal polarizationEight-cell stageLineage segregationMammalian developmentTrophectoderm specificationTE fateApical enrichmentApical accumulationDistinct lineagesApical domainApolar cellsPar complexPolarized cellsRNA interferenceMolecular mechanismsF-actinHuman embryosCell stageEmbryosDomain formationIndividual culture leads to decreased blastocyst formation but does not affect pregnancy outcomes in the setting of a single, vitrified-warmed euploid blastocyst transfer
Glatthorn HN, Hanson BM, Kim JG, Herlihy NS, Klimczak AM, Hong KH, Seli E, Scott RT. Individual culture leads to decreased blastocyst formation but does not affect pregnancy outcomes in the setting of a single, vitrified-warmed euploid blastocyst transfer. Journal Of Assisted Reproduction And Genetics 2021, 38: 2157-2164. PMID: 34086147, PMCID: PMC8417170, DOI: 10.1007/s10815-021-02252-8.Peer-Reviewed Original ResearchConceptsEuploid blastocyst transferIntracytoplasmic sperm injectionPregnancy outcomesBlastocyst transferSignificant differencesBlastocyst formationSingle embryo transferMann-Whitney U testPreimplantation genetic testingLogistic regression modelsOvarian stimulationClinical miscarriageEuploid blastocystsSperm injectionEuploid embryosEmbryo transferTrophectoderm biopsyGenetic testingGroup embryo cultureU testSame time frameDecreased rateOutcomesMiscarriageFertilization rateEvaluation of genome-wide DNA methylation profile of human embryos with different developmental competences
Yang M, Tao X, Scott K, Zhan Y, Scott RT, Seli E. Evaluation of genome-wide DNA methylation profile of human embryos with different developmental competences. Human Reproduction 2021, 36: 1682-1690. PMID: 33846747, DOI: 10.1093/humrep/deab074.Peer-Reviewed Original ResearchMeSH KeywordsAdultAneuploidyBlastocystDNA MethylationEmbryo Culture TechniquesEmbryo ImplantationFemaleHumansPregnancyPreimplantation DiagnosisConceptsWhole-genome bisulfite sequencingDNA methylation profilesGenome-wide DNA methylation levelsGenome-wide DNA methylation profilesGenome-wide methylation levelsDNA methylation levelsMethylation profilesMethylation levelsDNA methylationGenome-wide DNA methylome analysisWhole-genome sequencing librariesDifferent DNA methylation profilesEpigenetic regulatory mechanismsDifferent developmental potentialEmbryonic competenceDNA methylome analysisPre-implantation embryo developmentInvolved chromosomeTrophectoderm biopsy samplesDifferentiation of cellsHuman preimplantation embryosCopy number varianceChromosomal scaleLower methylation levelsEmbryo identityNoninvasive preimplantation genetic testing for aneuploidy exhibits high rates of deoxyribonucleic acid amplification failure and poor correlation with results obtained using trophectoderm biopsy
Hanson BM, Tao X, Hong KH, Comito CE, Pangasnan R, Seli E, Jalas C, Scott RT. Noninvasive preimplantation genetic testing for aneuploidy exhibits high rates of deoxyribonucleic acid amplification failure and poor correlation with results obtained using trophectoderm biopsy. Fertility And Sterility 2021, 115: 1461-1470. PMID: 33745720, DOI: 10.1016/j.fertnstert.2021.01.028.Peer-Reviewed Original ResearchMeSH KeywordsAneuploidyBlastocystCulture MediaEmbryo Culture TechniquesEmbryo ImplantationEmbryo TransferFemaleFertilityHigh-Throughput Nucleotide SequencingHumansInfertilityLive BirthMaleNoninvasive Prenatal TestingNucleic Acid Amplification TechniquesPredictive Value of TestsPregnancyPregnancy RatePreimplantation DiagnosisReproducibility of ResultsSperm Injections, IntracytoplasmicTime FactorsTreatment OutcomeWhole Genome SequencingConceptsPreimplantation genetic testingGenetic testingBiopsy resultsProspective cohort study SETTINGTrophectoderm biopsyTE biopsyNoninvasive preimplantation genetic testingCohort study SETTINGNext-generation sequencingHigh ratePrimary outcomeClinical outcomesMAIN OUTCOMEBiopsyStudy settingAmplification failureDiscordant resultsClinical applicabilityPrivate practiceNiPGTAvailable kitsCulture mediumShort durationOutcomesCulture medium samples
2018
Metabolic imaging with the use of fluorescence lifetime imaging microscopy (FLIM) accurately detects mitochondrial dysfunction in mouse oocytes
Sanchez T, Wang T, Pedro MV, Zhang M, Esencan E, Sakkas D, Needleman D, Seli E. Metabolic imaging with the use of fluorescence lifetime imaging microscopy (FLIM) accurately detects mitochondrial dysfunction in mouse oocytes. Fertility And Sterility 2018, 110: 1387-1397. PMID: 30446247, PMCID: PMC6289735, DOI: 10.1016/j.fertnstert.2018.07.022.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedComputer SystemsEmbryo Culture TechniquesEmbryo, MammalianEmbryonic DevelopmentEndopeptidase ClpFemaleFlavin-Adenine DinucleotideFluorescenceMaleMaternal AgeMiceMice, Inbred C57BLMice, KnockoutMicroscopy, FluorescenceMitochondriaMolecular ImagingNADOocytesReactive Oxygen SpeciesConceptsBlastocyst development rateOocyte dysfunctionReactive oxygen species levelsFlavin adenine dinucleotide (FAD) autofluorescenceMetabolic dysfunctionOxygen species levelsYoung miceMetabolic parametersOld miceMAIN OUTCOMEGlobal knockoutDysfunctionNoninvasive toolNormal oocytesMetabolic imagingMitochondrial dysfunctionMiceOld oocytesFLIM parametersROS levelsMetabolic differencesMitochondrial functionNicotinamide adenine dinucleotide dehydrogenaseIndividual oocytesWild-type oocytesDiminished ovarian reserve and poor response to stimulation in patients <38 years old: a quantitative but not qualitative reduction in performance
Morin SJ, Patounakis G, Juneau CR, Neal SA, Scott RT, Seli E. Diminished ovarian reserve and poor response to stimulation in patients <38 years old: a quantitative but not qualitative reduction in performance. Human Reproduction 2018, 33: 1489-1498. PMID: 30010882, DOI: 10.1093/humrep/dey238.Peer-Reviewed Original ResearchMeSH KeywordsAdultAge FactorsAneuploidyBlastomeresDatabases, FactualEmbryo Culture TechniquesEmbryo TransferFemaleFertility Agents, FemaleFertilization in VitroHumansInfertility, FemaleLive BirthOvarian ReserveOvaryOvulationOvulation InductionPregnancyPregnancy RateRetrospective StudiesTreatment OutcomeConceptsPoor oocyte qualityOvarian reserveLive birth rateDiminished ovarian reserveOocyte yieldOocyte qualityBlastulation rateAMH levelsPoor respondersPoor responseFollicular depletionBirth rateEmbryo transferAneuploidy rateLower live birth ratesYoung womenSTUDY FUNDING/COMPETINGGood prognosis patientsOvarian reserve parametersOvarian reserve testingYoung poor respondersPoor ovarian responseRetrospective cohort studyLower oocyte yieldInterquartile range
2016
IVF
Seli E, Garcia-Velasco J. IVF. Current Opinion In Obstetrics & Gynecology 2016, 28: 149-150. PMID: 27077470, DOI: 10.1097/gco.0000000000000275.Peer-Reviewed Original ResearchCryopreservationEmbryo Culture TechniquesEmbryo TransferFemaleFertilization in VitroHumansMalePregnancy
2010
Perspectives on emerging biomarkers for non-invasive assessment of embryo viability in assisted reproduction.
Aydiner F, Yetkin CE, Seli E. Perspectives on emerging biomarkers for non-invasive assessment of embryo viability in assisted reproduction. 2010, 10: 206-15. PMID: 20196727, DOI: 10.2174/156652410790963349.Peer-Reviewed Original Research
2009
Noninvasive metabolomic profiling as an adjunct to morphology for noninvasive embryo assessment in women undergoing single embryo transfer
Seli E, Vergouw CG, Morita H, Botros L, Roos P, Lambalk CB, Yamashita N, Kato O, Sakkas D. Noninvasive metabolomic profiling as an adjunct to morphology for noninvasive embryo assessment in women undergoing single embryo transfer. Fertility And Sterility 2009, 94: 535-542. PMID: 19589524, DOI: 10.1016/j.fertnstert.2009.03.078.Peer-Reviewed Original ResearchConceptsSingle embryo transferDay 3 embryosDay 2Embryo transferImplantation rateEmbryo culture mediumMetabolomic profilingPositive fetal cardiac activityAssisted reproductive technology programViability indexNoninvasive metabolomic profilingFetal cardiac activityReproductive technology programHuman embryo culture mediaRetrospective studyMAIN OUTCOMEDay 3Morphology gradeBlinded analysisCardiac activityReproductive potentialEmbryo assessmentCulture mediumBlinded validationWomen
2008
Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization
Seli E, Botros L, Sakkas D, Burns DH. Noninvasive metabolomic profiling of embryo culture media using proton nuclear magnetic resonance correlates with reproductive potential of embryos in women undergoing in vitro fertilization. Fertility And Sterility 2008, 90: 2183-2189. PMID: 18842260, DOI: 10.1016/j.fertnstert.2008.07.1739.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkersCulture MediaEmbryo Culture TechniquesEmbryo ImplantationEmbryo TransferEmbryo, MammalianEmbryonic DevelopmentFemaleFertilization in VitroHumansLive BirthMagnetic Resonance SpectroscopyMetabolomicsPredictive Value of TestsPregnancyPregnancy RateProtonsRetrospective StudiesSensitivity and SpecificityTreatment OutcomeConceptsEmbryo culture mediumNoninvasive metabolomic profilingMetabolomic profilesMetabolomic profilingReproductive technology programViability indexNuclear magnetic resonance metabolomic profilingRetrospective studyART treatmentMAIN OUTCOMEReproductive outcomesCulture mediumDay 3NMR metabolomic profilesGlutamate concentrationMultivariate analysisPregnancyNoninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study
Scott R, Seli E, Miller K, Sakkas D, Scott K, Burns DH. Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study. Fertility And Sterility 2008, 90: 77-83. PMID: 18281045, DOI: 10.1016/j.fertnstert.2007.11.058.Peer-Reviewed Original ResearchConceptsMetabolomic profilingProspective blinded pilot studyNoninvasive metabolomic profilingEmbryonic reproductive potentialBlinded pilot studyReproductive technology programCharacteristic curve analysisHuman embryo culture mediaOverall diagnostic accuracyViability indexImplantation rateEmbryo culture mediumMAIN OUTCOMEDay 3Noninvasive assessmentDay 5Culture mediumDiagnostic accuracyPilot studyCurve analysisSimilar findingsReproductive potentialRapid noninvasive assessmentDelivery rateHuman embryos
2007
Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization
Seli E, Sakkas D, Scott R, Kwok SC, Rosendahl SM, Burns DH. Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization. Fertility And Sterility 2007, 88: 1350-1357. PMID: 17923129, DOI: 10.1016/j.fertnstert.2007.07.1390.Peer-Reviewed Original Research