2024
Leukocytospermia does not negatively impact outcomes in in vitro fertilization cycles with intracytoplasmic sperm injection and preimplantation genetic testing for aneuploidy: findings from 5435 cycles
Gill P, Puchalt N, Molinaro T, Werner M, Seli E, Hotaling J, Cheng P. Leukocytospermia does not negatively impact outcomes in in vitro fertilization cycles with intracytoplasmic sperm injection and preimplantation genetic testing for aneuploidy: findings from 5435 cycles. Journal Of Assisted Reproduction And Genetics 2024, 41: 1213-1219. PMID: 38642270, PMCID: PMC11143111, DOI: 10.1007/s10815-024-03085-x.Peer-Reviewed Original ResearchConceptsLive birth rateIntracytoplasmic sperm injectionClinical pregnancy rateSustained implantation ratesIn vitro fertilizationIn vitro fertilization cyclesPreimplantation genetic testingBlastulation ratePGT-AAneuploidy rateEmbryological outcomesSperm injectionGenetic testingEuploid embryo transferPrevalence of leukocytospermiaRetrospective cohort studyMale partner ageImplantation rateBaseline characteristicsEmbryo transferLeukocytospermiaFertilization cyclesCohort studyPregnancy ratePrimary outcome
2021
B-cell lymphoma 6 expression is not associated with live birth in a normal responder in vitro fertilization population
Klimczak AM, Herlihy NS, Scott CS, Hanson BM, Kim JG, Titus S, Seli E, Scott RT. B-cell lymphoma 6 expression is not associated with live birth in a normal responder in vitro fertilization population. Fertility And Sterility 2021, 117: 351-358. PMID: 34809978, DOI: 10.1016/j.fertnstert.2021.09.036.Peer-Reviewed Original ResearchConceptsB-cell lymphoma 6 (Bcl6) expressionLive birth groupLive birthsFertilization populationNormal respondersBirth groupProportion of patientsLive birth ratePopulation of patientsEuploid embryo transferCase-control studyEndometrial tissue samplesPreimplantation genetic testingGroup of womenIVF stimulationMean HSCOREIVF outcomesInfertility centerIVF successMAIN OUTCOMEGroup 2Group 1Embryo transferPatientsClinical decisionEmbryology outcomes after oocyte vitrification with super-cooled slush nitrogen are similar to outcomes with conventional liquid nitrogen: a randomized controlled trial
Hanson BM, Kim JG, Suarez SI, Ackerman BK, Comito CE, Pangasnan R, Seli E, Hong KH, Scott RT. Embryology outcomes after oocyte vitrification with super-cooled slush nitrogen are similar to outcomes with conventional liquid nitrogen: a randomized controlled trial. Fertility And Sterility 2021, 117: 106-114. PMID: 34654569, DOI: 10.1016/j.fertnstert.2021.08.043.Peer-Reviewed Original ResearchConceptsMetaphase II oocytesEmbryology outcomesOocyte survivalII oocytesOocyte donor cyclesOocyte survival ratePreimplantation genetic testingBlastocyst formationWhole chromosome aneuploidyChromosome aneuploidySecondary outcomesPrimary outcomeDonor cyclesDonor oocytesRate of fertilizationMAIN OUTCOMESurvival rateOocyte vitrificationTrophectoderm biopsyGenetic testingDonor spermPrivate practiceNext-generation sequencingOutcomesDemonstrable improvementHuman embryo polarization requires PLC signaling to mediate trophectoderm specification
Zhu M, Shahbazi M, Martin A, Zhang C, Sozen B, Borsos M, Mandelbaum RS, Paulson RJ, Mole MA, Esbert M, Titus S, Scott RT, Campbell A, Fishel S, Gradinaru V, Zhao H, Wu K, Chen ZJ, Seli E, de los Santos MJ, Goetz M. Human embryo polarization requires PLC signaling to mediate trophectoderm specification. ELife 2021, 10: e65068. PMID: 34569938, PMCID: PMC8514238, DOI: 10.7554/elife.65068.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdultBody PatterningCell DifferentiationCell LineageCell PolarityEmbryo Culture TechniquesEmbryo, MammalianFemaleGATA3 Transcription FactorGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicHumansPhosphoinositide Phospholipase CPhospholipase C betaPregnancySignal TransductionTime FactorsYoung AdultConceptsApical domain formationInner cell massEmbryo polarizationPhospholipase CFirst lineage segregationApico-basal polarizationEight-cell stageLineage segregationMammalian developmentTrophectoderm specificationTE fateApical enrichmentApical accumulationDistinct lineagesApical domainApolar cellsPar complexPolarized cellsRNA interferenceMolecular mechanismsF-actinHuman embryosCell stageEmbryosDomain formationCumulus cells of euploid versus whole chromosome 21 aneuploid embryos reveal differentially expressed genes
Tiegs AW, Titus S, Mehta S, Garcia-Milian R, Seli E, Scott RT. Cumulus cells of euploid versus whole chromosome 21 aneuploid embryos reveal differentially expressed genes. Reproductive BioMedicine Online 2021, 43: 614-626. PMID: 34417138, DOI: 10.1016/j.rbmo.2021.06.015.Peer-Reviewed Original ResearchConceptsSerum response factorCumulus cellsDifferential gene expressionRNA sequencing analysisGene expression analysisIngenuity Pathway AnalysisCellular communication network factor 1Embryo developmental competenceExpression analysisPreimplantation embryo qualityGene expressionPathway analysisSegment polarity protein 2Sequencing analysisGenesProtein 2Response factorTrisomy 21Factor 1Developmental competenceAneuploid embryosReal-time polymerase chain reaction assaysDevelopment of biomarkersEmbryosCellsIndividual culture leads to decreased blastocyst formation but does not affect pregnancy outcomes in the setting of a single, vitrified-warmed euploid blastocyst transfer
Glatthorn HN, Hanson BM, Kim JG, Herlihy NS, Klimczak AM, Hong KH, Seli E, Scott RT. Individual culture leads to decreased blastocyst formation but does not affect pregnancy outcomes in the setting of a single, vitrified-warmed euploid blastocyst transfer. Journal Of Assisted Reproduction And Genetics 2021, 38: 2157-2164. PMID: 34086147, PMCID: PMC8417170, DOI: 10.1007/s10815-021-02252-8.Peer-Reviewed Original ResearchConceptsEuploid blastocyst transferIntracytoplasmic sperm injectionPregnancy outcomesBlastocyst transferSignificant differencesBlastocyst formationSingle embryo transferMann-Whitney U testPreimplantation genetic testingLogistic regression modelsOvarian stimulationClinical miscarriageEuploid blastocystsSperm injectionEuploid embryosEmbryo transferTrophectoderm biopsyGenetic testingGroup embryo cultureU testSame time frameDecreased rateOutcomesMiscarriageFertilization rateEvaluation of genome-wide DNA methylation profile of human embryos with different developmental competences
Yang M, Tao X, Scott K, Zhan Y, Scott RT, Seli E. Evaluation of genome-wide DNA methylation profile of human embryos with different developmental competences. Human Reproduction 2021, 36: 1682-1690. PMID: 33846747, DOI: 10.1093/humrep/deab074.Peer-Reviewed Original ResearchConceptsWhole-genome bisulfite sequencingDNA methylation profilesGenome-wide DNA methylation levelsGenome-wide DNA methylation profilesGenome-wide methylation levelsDNA methylation levelsMethylation profilesMethylation levelsDNA methylationGenome-wide DNA methylome analysisWhole-genome sequencing librariesDifferent DNA methylation profilesEpigenetic regulatory mechanismsDifferent developmental potentialEmbryonic competenceDNA methylome analysisPre-implantation embryo developmentInvolved chromosomeTrophectoderm biopsy samplesDifferentiation of cellsHuman preimplantation embryosCopy number varianceChromosomal scaleLower methylation levelsEmbryo identityAnalysis of female demographics in the United States: life expectancy, education, employment, family building decisions, and fertility service utilization
Esencan E, Simsek B, Seli E. Analysis of female demographics in the United States: life expectancy, education, employment, family building decisions, and fertility service utilization. Current Opinion In Obstetrics & Gynecology 2021, 33: 170-177. PMID: 33797423, DOI: 10.1097/gco.0000000000000704.Peer-Reviewed Original ResearchConceptsReproductive technology treatmentMedian agePregnancy attemptsART cyclesMean ageService utilizationPregnancy rateFemale fertility ratesFamily-building decisionsFertility servicesFraction of womenTechnology treatmentDonor eggsFertility rateFirst birthWomenLife expectancyAgeFemale demographicDiagnosisMarried womenThe Appraisal of Body Content (ABC) trial: Increased male or female adiposity does not significantly impact in vitro fertilization laboratory or clinical outcomes
Kim J, Patounakis G, Juneau C, Morin S, Neal S, Bergh P, Seli E, Scott R. The Appraisal of Body Content (ABC) trial: Increased male or female adiposity does not significantly impact in vitro fertilization laboratory or clinical outcomes. Fertility And Sterility 2021, 116: 444-452. PMID: 33581854, DOI: 10.1016/j.fertnstert.2020.12.037.Peer-Reviewed Original ResearchConceptsBody mass indexBioelectric impedance analysisLive birth rateLow birth weightClinical outcomesMiscarriage rateBlastocyst formation rateEuploidy rateImplantation rateBirth weightLow birth weight/Low birth weight infantsNormal weight patientsBirth weight infantsProspective cohort studyImpact of obesityFertilization laboratoryBirth rateFertilization rateBF categoriesWeight patientsMaternal obesityWeight infantsCohort studyObese women
2020
Shorter telomere length of white blood cells is associated with higher rates of aneuploidy among infertile women undergoing in vitro fertilization
Hanson BM, Tao X, Zhan Y, Kim JG, Klimczak AM, Herlihy NS, Scott RT, Seli E. Shorter telomere length of white blood cells is associated with higher rates of aneuploidy among infertile women undergoing in vitro fertilization. Fertility And Sterility 2020, 115: 957-965. PMID: 33272640, DOI: 10.1016/j.fertnstert.2020.09.164.Peer-Reviewed Original ResearchConceptsWhite blood cellsPatient ageInfertile womenCumulus cellsBlood cellsEmbryonic aneuploidyPeak estradiol levelsAntimüllerian hormone levelsProspective cohort studyTelomere lengthNumber of oocytesShorter telomere lengthReal-time polymerase chain reactionQuantitative real-time polymerase chain reactionHigh rateWarrants further investigationTelomere length assessmentSignificant overlap existsCohort studyOocyte retrievalSingle centerEstradiol levelsInfertile populationMaternal agePolymerase chain reactionRate of true recurrent implantation failure is low: results of three successive frozen euploid single embryo transfers
Pirtea P, De Ziegler D, Tao X, Sun L, Zhan Y, Ayoubi JM, Seli E, Franasiak JM, Scott RT. Rate of true recurrent implantation failure is low: results of three successive frozen euploid single embryo transfers. Fertility And Sterility 2020, 115: 45-53. PMID: 33077239, DOI: 10.1016/j.fertnstert.2020.07.002.Peer-Reviewed Original ResearchConceptsRecurrent implantation failureEuploid single-embryo transferLive birth rateSingle embryo transferImplantation rateImplantation failureEmbryo transferCumulative live birth rateSustained implantation ratesRetrospective cohort studyKaplan-Meier curvesReproductive technology centerDifference of outcomeBirth rateCumulative implantation rateLogistic regression modelsPositive heartbeatClinical pregnancyCohort studyMiscarriage rateMean ageEuploid blastocystsNormal uterusMAIN OUTCOMEGestational carrierA multicenter, prospective, blinded, nonselection study evaluating the predictive value of an aneuploid diagnosis using a targeted next-generation sequencing–based preimplantation genetic testing for aneuploidy assay and impact of biopsy
Tiegs AW, Tao X, Zhan Y, Whitehead C, Kim J, Hanson B, Osman E, Kim TJ, Patounakis G, Gutmann J, Castelbaum A, Seli E, Jalas C, Scott RT. A multicenter, prospective, blinded, nonselection study evaluating the predictive value of an aneuploid diagnosis using a targeted next-generation sequencing–based preimplantation genetic testing for aneuploidy assay and impact of biopsy. Fertility And Sterility 2020, 115: 627-637. PMID: 32863013, DOI: 10.1016/j.fertnstert.2020.07.052.Peer-Reviewed Original ResearchConceptsPreimplantation genetic testingSustained implantationPredictive valueGenetic testingClinical outcomesTrophectoderm biopsyAge-matched control groupImpact of biopsyPGT-A resultsAntral follicle countRecurrent pregnancy lossBody mass indexDetectable adverse impactAneuploid resultsSecondary outcomesFertilization cyclesFollicle countPrimary outcomeMass indexUsable blastocystsBlastocyst transferFertility centerPregnancy lossClinical error ratesStudy groupThe appraisal of body content (ABC) trial: obesity does not significantly impact gamete production in infertile men and women
Kim J, Juneau C, Patounakis G, Morin S, Neal S, Seli E, Scott R. The appraisal of body content (ABC) trial: obesity does not significantly impact gamete production in infertile men and women. Journal Of Assisted Reproduction And Genetics 2020, 37: 2733-2742. PMID: 32827101, PMCID: PMC7642175, DOI: 10.1007/s10815-020-01930-3.Peer-Reviewed Original ResearchConceptsBody mass indexLower serum AMHLower serum FSHOvarian reserve parametersPresence of PCOSControlled ovarian hyperstimulationNormal-weight counterpartsMature oocytesPercent body fatBF categoriesOvarian hyperstimulationSerum AMHOvarian reservePCOS diagnosisSerum FSHIVF outcomesLess muscle massOvarian responseMass indexFemale obesityInfertility treatmentReserve parametersBody fatInfertile menObesityAnalysis of accessible chromatin landscape in the inner cell mass and trophectoderm of human blastocysts
Yang M, Tao X, Titus S, Zhao T, Scott RT, Seli E. Analysis of accessible chromatin landscape in the inner cell mass and trophectoderm of human blastocysts. Molecular Human Reproduction 2020, 26: 702-711. PMID: 32663300, DOI: 10.1093/molehr/gaaa048.Peer-Reviewed Original ResearchConceptsInner cell massHuman preimplantation embryosEpigenetic regulationPreimplantation embryosCell lineage specificationChromatin accessibility landscapeAccessible chromatin landscapePreimplantation human blastocystsEarly embryonic developmentTranscription start siteCell massEarly embryo developmentHuman blastocystsDistal regionChromatin landscapeAccessible chromatinLineage specificationChromatin structureLow DNA inputATAC-seqTrophectoderm differentiationChromosome reorganizationAccessibility landscapeHuman embryonic materialEmbryonic developmentEffects of intraovarian injection of autologous platelet rich plasma on ovarian reserve and IVF outcome parameters in women with primary ovarian insufficiency
Cakiroglu Y, Saltik A, Yuceturk A, Karaosmanoglu O, Kopuk SY, Scott RT, Tiras B, Seli E. Effects of intraovarian injection of autologous platelet rich plasma on ovarian reserve and IVF outcome parameters in women with primary ovarian insufficiency. Aging 2020, 12: 10211-10222. PMID: 32507764, PMCID: PMC7346073, DOI: 10.18632/aging.103403.Peer-Reviewed Original ResearchConceptsAutologous platelet-rich plasmaPrimary ovarian insufficiencyPlatelet-rich plasmaIVF outcome parametersAntral follicle countIntraovarian injectionOvarian reservePRP injectionAntimullerian hormoneOutcome parametersOvarian insufficiencyRich plasmaSerum antimullerian hormoneUnderwent embryo transferExperimental treatment optionsESHRE criteriaSustained implantationOvarian stimulationSerum follicleFollicle countPRP treatmentTreatment optionsFertilization outcomeAntral folliclesEmbryo transferMitochondrial DNA content is not predictive of reproductive competence in euploid blastocysts
Scott RT, Sun L, Zhan Y, Marin D, Tao X, Seli E. Mitochondrial DNA content is not predictive of reproductive competence in euploid blastocysts. Reproductive BioMedicine Online 2020, 41: 183-190. PMID: 32600944, DOI: 10.1016/j.rbmo.2020.04.011.Peer-Reviewed Original ResearchConceptsRelative mtDNA copy numberSingle embryo transferMitochondrial DNA copy numberMtDNA copy numberEmbryo transferSuccessful single embryo transferDNA copy numberRelative mtDNA levelsHuman blastocystsReal-time polymerase chain reactionQuantitative real-time polymerase chain reactionReproductive competenceIVF outcomesMaternal ageOngoing implantationPolymerase chain reactionEuploid blastocystsMitochondrial DNA contentReproductive outcomesSame patientClinical decisionTrophectoderm biopsySame cohortAmount of mtDNACopy numberSperm Mitochondrial DNA Copy Number Is Not a Predictor of Intracytoplasmic Sperm Injection (ICSI) Cycle Outcomes
Tiegs AW, Tao X, Landis J, Zhan Y, Franasiak JM, Seli E, Wells D, Fragouli E, Scott RT. Sperm Mitochondrial DNA Copy Number Is Not a Predictor of Intracytoplasmic Sperm Injection (ICSI) Cycle Outcomes. Reproductive Sciences 2020, 27: 1350-1356. PMID: 31994001, DOI: 10.1007/s43032-020-00163-0.Peer-Reviewed Original ResearchConceptsSperm mitochondrial DNA copy numberLive birth rateQuantitative real-time polymerase chain reactionMitochondrial DNA copy numberICSI cyclesBirth rateIntracytoplasmic sperm injection cycle outcomesBlastocyst developmentDay-3 embryo biopsyMtDNA-CNDNA copy numberBlastocyst euploidy ratesMixed-effects logistic regressionReal-time polymerase chain reactionSperm samplesEffects logistic regressionBlastocyst euploidyCohort studyPrimary outcomeSecondary outcomesInfertility centerCycle outcomeEuploidy rateMaternal ageInfertile population
2019
Cumulus cells have longer telomeres than leukocytes in reproductive-age women
Lara-Molina EE, Franasiak JM, Marin D, Tao X, Díaz-Gimeno P, Florensa M, Martin M, Seli E, Pellicer A. Cumulus cells have longer telomeres than leukocytes in reproductive-age women. Fertility And Sterility 2019, 113: 217-223. PMID: 31594634, DOI: 10.1016/j.fertnstert.2019.08.089.Peer-Reviewed Original ResearchConceptsLeukocyte telomere lengthGranulosa cellsCumulus cellsTelomere lengthAssisted reproductive technology centerRelative leukocyte telomere lengthProspective noninterventional studyAntimüllerian hormone levelsAntral follicle countReproductive-age womenReproductive technology centerLonger telomeresNoninterventional studyFollicle countMean ageHormone levelsFollicular environmentMAIN OUTCOMEMature folliclesStudy subjectsSignificant associationFollicular cellsMean numberMature oocytesSignificant differencesGonadotropin-Releasing Hormone Analogs for Gonadal Protection During Gonadotoxic Chemotherapy: A Systematic Review and Meta-Analysis
Sofiyeva N, Siepmann T, Barlinn K, Seli E, Ata B. Gonadotropin-Releasing Hormone Analogs for Gonadal Protection During Gonadotoxic Chemotherapy: A Systematic Review and Meta-Analysis. Reproductive Sciences 2019, 26: 939-953. PMID: 30270741, DOI: 10.1177/1933719118799203.Peer-Reviewed Original ResearchConceptsGonadotropin-releasing hormone analogueSystematic reviewGonadotoxic chemotherapyHormone analogueRisk ratioGnRH agonist-treated patientsGonadotropin-Releasing Hormone AnalogLow-quality evidenceReproductive-aged womenConfidence intervalsSignificant protective effectGonadoprotective effectPresent systematic reviewGnRHa cotreatmentGonadal protectionSupradiaphragmatic radiotherapyCohort studyTrials RegistryGnRHa administrationMenstrual resumptionAutoimmune diseasesSubgroup analysisBreast cancerProtective effectHematological malignanciesDiminished ovarian reserve versus ovarian aging: overlaps and differences.
Ata B, Seyhan A, Seli E. Diminished ovarian reserve versus ovarian aging: overlaps and differences. Current Opinion In Obstetrics & Gynecology 2019, 31: 139-147. PMID: 30870184, DOI: 10.1097/gco.0000000000000536.Peer-Reviewed Original ResearchConceptsNormal ovarian reserveOvarian reservePregnancy lossOocyte qualityReproductive technology cyclesAge-matched womenDiminished ovarian reserveLow ovarian reserveFetal chromosomal abnormalitiesQuantitative declineFecundity of womenOvarian stimulationNatural conceptionOvarian agingPoor responseART treatmentAged womenYoung womenChromosomal abnormalitiesAvailable evidenceDecreased numberQualitative declineOocyte poolWomenBlastocyst development