2024
Deciphering the HLA-E immunopeptidome with mass spectrometry: an opportunity for universal mRNA vaccines and T-cell-directed immunotherapies
Weitzen M, Shahbazy M, Kapoor S, Caron E. Deciphering the HLA-E immunopeptidome with mass spectrometry: an opportunity for universal mRNA vaccines and T-cell-directed immunotherapies. Frontiers In Immunology 2024, 15: 1442783. PMID: 39301027, PMCID: PMC11410602, DOI: 10.3389/fimmu.2024.1442783.Peer-Reviewed Original ResearchMass spectrometryHLA-EHLA moleculesT-cell-directed immunotherapyCD8+ T cellsNovel cell surface antigensNon-classical HLA-EHLA-E moleculesCell surface antigensPeptides to CD8T cellsMRNA vaccinesTherapeutic efficacySurface antigensImmunotherapyMoleculesSpectrometryTherapeutic targetImmunopeptidomePathogen-derivedCD8Peptide repertoirePeptide fragmentsHLAMinimal polymorphism
2023
Unlocking the potential of microfluidics in mass spectrometry-based immunopeptidomics for tumor antigen discovery
Stutzmann C, Peng J, Wu Z, Savoie C, Sirois I, Thibault P, Wheeler A, Caron E. Unlocking the potential of microfluidics in mass spectrometry-based immunopeptidomics for tumor antigen discovery. Cell Reports Methods 2023, 3: 100511. PMID: 37426761, PMCID: PMC10326451, DOI: 10.1016/j.crmeth.2023.100511.Peer-Reviewed Original ResearchConceptsSingle-cell proteomicsMicrofluidic technologyPotential of microfluidicsMass spectrometrySample preparationDroplet microfluidicsDigital microfluidicsMicrofluidic methodPhysical moleculesMicrofluidicsHigh sensitivityReproducible mannerPillar arraysRecent advancesSpectrometryMoleculesImmunopeptidomicsPowerful toolProteomicsPreparationMSPeptidesSolution
2017
Precise Temporal Profiling of Signaling Complexes in Primary Cells Using SWATH Mass Spectrometry
Caron E, Roncagalli R, Hase T, Wolski W, Choi M, Menoita M, Durand S, García-Blesa A, Fierro-Monti I, Sajic T, Heusel M, Weiss T, Malissen M, Schlapbach R, Collins B, Ghosh S, Kitano H, Aebersold R, Malissen B, Gstaiger M. Precise Temporal Profiling of Signaling Complexes in Primary Cells Using SWATH Mass Spectrometry. Cell Reports 2017, 18: 3219-3226. PMID: 28355572, PMCID: PMC5382234, DOI: 10.1016/j.celrep.2017.03.019.Peer-Reviewed Original ResearchConceptsSWATH mass spectrometryProtein interactionsProtein interaction dynamicsPrimary cellsTissue-specific contextDifferential protein expressionCell-signaling eventsInteraction proteomeProtein Grb2Cellular functionsMouse geneticsCell signalingAffinity purificationMammalian tissuesDevelopmental stagesTemporal profilingSpatiotemporal organizationMass spectrometryFundamental processesProtein expressionCell populationsRobust workflowCellsInteraction dynamicsGrb2
2016
Contribution of Mass Spectrometry-Based Proteomics to the Understanding of TNF‑α Signaling
Ciuffa R, Caron E, Leitner A, Uliana F, Gstaiger M, Aebersold R. Contribution of Mass Spectrometry-Based Proteomics to the Understanding of TNF‑α Signaling. Journal Of Proteome Research 2016, 16: 14-33. PMID: 27762135, DOI: 10.1021/acs.jproteome.6b00728.Peer-Reviewed Original ResearchConceptsTNF-α signalingProteomics researchDimeric transcription factorsComplex signaling cascadesSystems-level understandingProteome levelProteomic landscapeCellular processesTranscription factorsSignaling cascadesNF-κBSignalingLevel understandingMass spectrometryProteomeActivationProteomicsSpite of decadesCytokines IL-1βDifferentiationLimited knowledgePathwayCascadeFamilyTNF
2015
An open-source computational and data resource to analyze digital maps of immunopeptidomes
Caron E, Espona L, Kowalewski D, Schuster H, Ternette N, Alpízar A, Schittenhelm R, Ramarathinam S, Arlehamn C, Koh C, Gillet L, Rabsteyn A, Navarro P, Kim S, Lam H, Sturm T, Marcilla M, Sette A, Campbell D, Deutsch E, Moritz R, Purcell A, Rammensee H, Stevanovic S, Aebersold R. An open-source computational and data resource to analyze digital maps of immunopeptidomes. ELife 2015, 4: e07661. PMID: 26154972, PMCID: PMC4507788, DOI: 10.7554/elife.07661.Peer-Reviewed Original Research
2014
A repository of assays to quantify 10,000 human proteins by SWATH-MS
Rosenberger G, Koh CC, Guo T, Röst HL, Kouvonen P, Collins BC, Heusel M, Liu Y, Caron E, Vichalkovski A, Faini M, Schubert OT, Faridi P, Ebhardt HA, Matondo M, Lam H, Bader SL, Campbell DS, Deutsch EW, Moritz RL, Tate S, Aebersold R. A repository of assays to quantify 10,000 human proteins by SWATH-MS. Scientific Data 2014, 1: 140031. PMID: 25977788, PMCID: PMC4322573, DOI: 10.1038/sdata.2014.31.Peer-Reviewed Original Research