2021
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis.
Sirois I, Isabelle M, Duquette J, Saab F, Caron E. Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis. Journal Of Visualized Experiments 2021 PMID: 34723952, DOI: 10.3791/63052.Peer-Reviewed Original ResearchConceptsImmunopeptidomics workflowsComposition of peptidesSample preparation procedureQuality control samplesMass spectrometry analysisPreparation procedureElution efficiencySample preparationSpectrometry analysisPeptidesImmunoaffinity purificationReagentsMoleculesComplexesPreparationPurificationStepMHC-peptide complexesControl samplesImmunopeptidomicsNew protocolReproducibilityEfficiencyBeadsTechnology platform
2010
Deletion of Immunoproteasome Subunits Imprints on the Transcriptome and Has a Broad Impact on Peptides Presented by Major Histocompatibility Complex I molecules*
de Verteuil D, Muratore-Schroeder T, Granados D, Fortier M, Hardy M, Bramoullé A, Caron É, Vincent K, Mader S, Lemieux S, Thibault P, Perreault C. Deletion of Immunoproteasome Subunits Imprints on the Transcriptome and Has a Broad Impact on Peptides Presented by Major Histocompatibility Complex I molecules*. Molecular & Cellular Proteomics 2010, 9: 2034-2047. PMID: 20484733, PMCID: PMC2938112, DOI: 10.1074/mcp.m900566-mcp200.Peer-Reviewed Original ResearchConceptsMIP repertoireLabel-free quantitative proteomics approachComplex ICell type-specific signaturesBasic cellular processesUnstructured protein regionsProteasome-mediated proteolysisQuantitative proteomics approachSet of genesPrimary mouse dendritic cellsJawed vertebratesRegulated genesCellular processesProteomic approachMass spectrometry analysisGene transcriptionBioinformatics analysisChromosome 4Protein regionsPrimary cellsGenesCleavage preferenceSpectrometry analysisCell functionPeptidomics studies