2021
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis.
Sirois I, Isabelle M, Duquette J, Saab F, Caron E. Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis. Journal Of Visualized Experiments 2021 PMID: 34723952, DOI: 10.3791/63052.Peer-Reviewed Original ResearchMeSH KeywordsHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHLA AntigensHumansMass SpectrometryPeptidesReproducibility of ResultsConceptsImmunopeptidomics workflowsComposition of peptidesSample preparation procedureQuality control samplesMass spectrometry analysisPreparation procedureElution efficiencySample preparationSpectrometry analysisPeptidesImmunoaffinity purificationReagentsMoleculesComplexesPreparationPurificationStepMHC-peptide complexesControl samplesImmunopeptidomicsNew protocolReproducibilityEfficiencyBeadsTechnology platform
2017
Precise Temporal Profiling of Signaling Complexes in Primary Cells Using SWATH Mass Spectrometry
Caron E, Roncagalli R, Hase T, Wolski W, Choi M, Menoita M, Durand S, García-Blesa A, Fierro-Monti I, Sajic T, Heusel M, Weiss T, Malissen M, Schlapbach R, Collins B, Ghosh S, Kitano H, Aebersold R, Malissen B, Gstaiger M. Precise Temporal Profiling of Signaling Complexes in Primary Cells Using SWATH Mass Spectrometry. Cell Reports 2017, 18: 3219-3226. PMID: 28355572, PMCID: PMC5382234, DOI: 10.1016/j.celrep.2017.03.019.Peer-Reviewed Original ResearchConceptsSWATH mass spectrometryProtein interactionsProtein interaction dynamicsPrimary cellsTissue-specific contextDifferential protein expressionCell-signaling eventsInteraction proteomeProtein Grb2Cellular functionsMouse geneticsCell signalingAffinity purificationMammalian tissuesDevelopmental stagesTemporal profilingSpatiotemporal organizationMass spectrometryFundamental processesProtein expressionCell populationsRobust workflowCellsInteraction dynamicsGrb2