2021
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis.
Sirois I, Isabelle M, Duquette J, Saab F, Caron E. Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis. Journal Of Visualized Experiments 2021 PMID: 34723952, DOI: 10.3791/63052.Peer-Reviewed Original ResearchMeSH KeywordsHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHLA AntigensHumansMass SpectrometryPeptidesReproducibility of ResultsConceptsImmunopeptidomics workflowsComposition of peptidesSample preparation procedureQuality control samplesMass spectrometry analysisPreparation procedureElution efficiencySample preparationSpectrometry analysisPeptidesImmunoaffinity purificationReagentsMoleculesComplexesPreparationPurificationStepMHC-peptide complexesControl samplesImmunopeptidomicsNew protocolReproducibilityEfficiencyBeadsTechnology platform
2019
The Human Immunopeptidome Project: A Roadmap to Predict and Treat Immune Diseases*
Vizcaíno J, Kubiniok P, Kovalchik K, Ma Q, Duquette J, Mongrain I, Deutsch E, Peters B, Sette A, Sirois I, Caron E. The Human Immunopeptidome Project: A Roadmap to Predict and Treat Immune Diseases*. Molecular & Cellular Proteomics 2019, 19: 31-49. PMID: 31744855, PMCID: PMC6944237, DOI: 10.1074/mcp.r119.001743.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAutoimmune DiseasesCohort StudiesHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHLA AntigensHumansImmunotherapyInfectionsMass SpectrometryNeoplasmsPeptidesPrecision MedicinePrognosis
2018
Minimal Information About an Immuno‐Peptidomics Experiment (MIAIPE)
Lill J, van Veelen P, Tenzer S, Admon A, Caron E, Elias J, Heck A, Marcilla M, Marino F, Müller M, Peters B, Purcell A, Sette A, Sturm T, Ternette N, Vizcaíno J, Bassani‐Sternberg M. Minimal Information About an Immuno‐Peptidomics Experiment (MIAIPE). Proteomics 2018, 18: 1800110. PMID: 29791771, PMCID: PMC6033177, DOI: 10.1002/pmic.201800110.Peer-Reviewed Original ResearchComputational BiologyDatabases, ProteinHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHumansPeptide FragmentsProteomicsSoftwareSpecimen Handling