2011
Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement
Isaacs FJ, Carr PA, Wang HH, Lajoie MJ, Sterling B, Kraal L, Tolonen AC, Gianoulis TA, Goodman DB, Reppas NB, Emig CJ, Bang D, Hwang SJ, Jewett MC, Jacobson JM, Church GM. Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement. Science 2011, 333: 348-353. PMID: 21764749, PMCID: PMC5472332, DOI: 10.1126/science.1205822.Peer-Reviewed Original ResearchConceptsGenome engineeringSynonymous codon substitutionsGenome engineering technologiesSynthetic lethal effectMegabase scaleCodon replacementsTAA codonCodon substitutionsRecombination frequencyCodon modificationGenetic landscapeEscherichia coliGenomeChromosomesCodonPrecise changesLethal effectsPrecise manipulationEngineering technologyNucleotidesColiPhenotypeTagsModification
2009
Programming cells by multiplex genome engineering and accelerated evolution
Wang HH, Isaacs FJ, Carr PA, Sun ZZ, Xu G, Forest CR, Church GM. Programming cells by multiplex genome engineering and accelerated evolution. Nature 2009, 460: 894-898. PMID: 19633652, PMCID: PMC4590770, DOI: 10.1038/nature08187.Peer-Reviewed Original Research
2006
RNA synthetic biology
Isaacs FJ, Dwyer DJ, Collins JJ. RNA synthetic biology. Nature Biotechnology 2006, 24: 545-554. PMID: 16680139, DOI: 10.1038/nbt1208.Peer-Reviewed Original ResearchConceptsRNA moleculesSynthetic biologyDiverse regulatory rolesRNA synthetic biologyNew biological functionsCellular control systemsSensors of intracellularMammalian systemsRNA componentBiological functionsGene expressionNatural versatilityCellular behaviorRegulatory roleRNA systemsEnvironmental stimuliBiological networksSmall moleculesBiologyNucleic acidsMoleculesYeastProteinBacteriaIntracellular
2004
Engineered riboregulators enable post-transcriptional control of gene expression
Isaacs FJ, Dwyer DJ, Ding C, Pervouchine DD, Cantor CR, Collins JJ. Engineered riboregulators enable post-transcriptional control of gene expression. Nature Biotechnology 2004, 22: 841-847. PMID: 15208640, DOI: 10.1038/nbt986.Peer-Reviewed Original ResearchConceptsStem-loop structureGene expressionPost-transcriptional controlPost-transcriptional regulation systemSmall noncoding RNAsTrans targetsCis-repressive sequenceRibosome bindingNoncoding RNAsEndogenous RNATarget genesCis sequencesRegulatory roleEscherichia coliRNABiological networksRiboregulatorsMechanistic actionGenesExpressionRecent studiesSequenceRegulation systemTranscriptionPromoter