2014
Improved bioactivity of G-rich triplex-forming oligonucleotides containing modified guanine bases
Rogers FA, Lloyd JA, Tiwari MK. Improved bioactivity of G-rich triplex-forming oligonucleotides containing modified guanine bases. Artificial DNA PNA & XNA 2014, 5: e27792. PMID: 25483840, PMCID: PMC4014521, DOI: 10.4161/adna.27792.Peer-Reviewed Original ResearchConceptsMobility gel shift assaysDNA double-strand breaksTarget sequenceGel shift assaysDouble-strand breaksGene-targeted mutagenesisActivation of apoptosisG-quadruplex formationGenomic modificationsDNA repairShift assaysReporter geneMolecular mechanismsPolypurine sequenceStrand breaksTriplex technologyTarget siteMutagenesisTriplex structureGenesGuanine baseTriplex formationGuanine basesSequenceAG30
2013
Triplex-induced DNA damage response.
Rogers FA, Tiwari MK. Triplex-induced DNA damage response. The Yale Journal Of Biology And Medicine 2013, 86: 471-8. PMID: 24348211, PMCID: PMC3848101.Peer-Reviewed Original ResearchConceptsDNA damage responseGenomic integrityDamage responseCellular pathwaysCellular DNA damage responseDNA damageAppropriate cellular responsesPro-apoptotic factorsCell cycle arrestDNA repairDNA strand breaksCellular responsesCycle arrestDamage sitesStrand breaksPathwayApoptosisHelical structureComplex seriesCentral roleTriplex formationXPDPhosphorylationΓH2AXDNAXPD-dependent activation of apoptosis in response to triplex-induced DNA damage
Tiwari M, Rogers FA. XPD-dependent activation of apoptosis in response to triplex-induced DNA damage. Nucleic Acids Research 2013, 41: 8979-8994. PMID: 23913414, PMCID: PMC3799437, DOI: 10.1093/nar/gkt670.Peer-Reviewed Original ResearchConceptsDouble-strand breaksStrand breaksGenomic integrityPro-apoptotic pathwaysHuman genomeTyrosine 142DNA repairDNA sequencesGenomic instabilityDNA strand breaksNoncanonical structuresCancer initiationDNA damageDamage sitesΓH2AX fociHelical distortionApoptosisTriplex structureCentral rolePathwayXPDGenomeTriplexesPhosphorylationΓH2AX
2011
XPD‐Dependent induction of apoptosis in cells with DNA containing triple helical repeats
Rogers F, Kaushik M. XPD‐Dependent induction of apoptosis in cells with DNA containing triple helical repeats. The FASEB Journal 2011, 25: 310.3-310.3. DOI: 10.1096/fasebj.25.1_supplement.310.3.Peer-Reviewed Original ResearchDNA repairH-DNAAltered helical structureNucleotide excision repair pathwayExcision repair pathwayTriple helical repeatsGenomic integrityPro-apoptotic pathwaysHuman genomeInduction of mutationsDNA sequencesRepair pathwaysHelical repeatBase pairsDNA damageIntricate balanceHelical distortionRepair synthesisTriplex structureApoptosisHelical structureCentral roleTriplex formationPathwayInduction
2009
Targeted correction of a thalassemia-associated β-globin mutation induced by pseudo-complementary peptide nucleic acids
Lonkar P, Kim KH, Kuan JY, Chin JY, Rogers FA, Knauert MP, Kole R, Nielsen PE, Glazer PM. Targeted correction of a thalassemia-associated β-globin mutation induced by pseudo-complementary peptide nucleic acids. Nucleic Acids Research 2009, 37: 3635-3644. PMID: 19364810, PMCID: PMC2699504, DOI: 10.1093/nar/gkp217.Peer-Reviewed Original ResearchConceptsBeta-globin geneNucleotide excision repair factorsAltered helical structureExcision repair factorsSingle base-pair modificationTriplex-forming peptide nucleic acidsPseudo-complementary peptide nucleic acidsDisease-related genesDonor DNA fragmentsComplementary DNA sequenceNucleic acidsProper splicingRepair factorsSite-specific bindingMammalian cellsSite-specific modificationDNA repairDNA sequencesGene targetingSecond intronDNA fragmentsHuman cellsTriple helix formationGene correctionHuman fibroblast cells
2002
Site-directed recombination via bifunctional PNA–DNA conjugates
Rogers FA, Vasquez KM, Egholm M, Glazer PM. Site-directed recombination via bifunctional PNA–DNA conjugates. Proceedings Of The National Academy Of Sciences Of The United States Of America 2002, 99: 16695-16700. PMID: 12461167, PMCID: PMC139206, DOI: 10.1073/pnas.262556899.Peer-Reviewed Original ResearchConceptsSite-directed recombinationDNA repairNucleotide excision repair factorsExcision repair factorsSite-specific DNASite-specific recombinationHuman cell-free extractsDonor DNA fragmentsRepair factorsMammalian cellsGenetic manipulationCell-free extractsTarget genesSite-directed mannerReporter geneStrand invasionPlasmid substratesDNA fragmentsDefective geneDonor DNAWatson-Crick base pairingDuplex DNAGenesTarget plasmidDNA