2016
Triplex structures induce DNA double strand breaks via replication fork collapse in NER deficient cells
Tiwari M, Adaku N, Peart N, Rogers FA. Triplex structures induce DNA double strand breaks via replication fork collapse in NER deficient cells. Nucleic Acids Research 2016, 44: 7742-7754. PMID: 27298253, PMCID: PMC5027492, DOI: 10.1093/nar/gkw515.Peer-Reviewed Original ResearchConceptsDouble-strand breaksReplication fork collapseFork collapseGenomic instabilityReplication-associated double-strand breaksDNA replication fork progressionNucleotide excision repair proteinsTriplex structureRobust DNA damage responseDNA double-strand breaksReplication fork progressionReplication fork stabilityStrand breaksReplication fork integrityXPC-deficient cellsDNA damage responseMirror repeat sequencesExcision repair proteinsMore DSBsNER-deficient cellsDamage recognition proteinsGenomic integrityDual phosphorylationFork stabilityFork integrity
2014
Improved bioactivity of G-rich triplex-forming oligonucleotides containing modified guanine bases
Rogers FA, Lloyd JA, Tiwari MK. Improved bioactivity of G-rich triplex-forming oligonucleotides containing modified guanine bases. Artificial DNA PNA & XNA 2014, 5: e27792. PMID: 25483840, PMCID: PMC4014521, DOI: 10.4161/adna.27792.Peer-Reviewed Original ResearchConceptsMobility gel shift assaysDNA double-strand breaksTarget sequenceGel shift assaysDouble-strand breaksGene-targeted mutagenesisActivation of apoptosisG-quadruplex formationGenomic modificationsDNA repairShift assaysReporter geneMolecular mechanismsPolypurine sequenceStrand breaksTriplex technologyTarget siteMutagenesisTriplex structureGenesGuanine baseTriplex formationGuanine basesSequenceAG30
2012
Local Delivery of Gene-Modifying Triplex-Forming Molecules to the Epidermis
Rogers FA, Hu RH, Milstone LM. Local Delivery of Gene-Modifying Triplex-Forming Molecules to the Epidermis. Journal Of Investigative Dermatology 2012, 133: 685-691. PMID: 23014335, PMCID: PMC3532560, DOI: 10.1038/jid.2012.351.Peer-Reviewed Original ResearchConceptsIntradermal deliveryHairless miceTransgenic miceBasal keratinocytesMethod of deliveryNuclei of keratinocytesIntraperitoneal administrationSitu gene correctionBack skinLocal deliveryMiceDisease-causing genesEpidermal keratinocytesKeratinocytesLocal administrationAdministrationDeliverySkinEpidermisDays
2005
Triplex-forming oligonucleotides as potential tools for modulation of gene expression.
Rogers FA, Lloyd JA, Glazer PM. Triplex-forming oligonucleotides as potential tools for modulation of gene expression. Anti-Cancer Agents In Medicinal Chemistry 2005, 5: 319-26. PMID: 16101484, DOI: 10.2174/1568011054222300.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNAGene Expression RegulationHumansMutagensNucleic Acid ConformationOligonucleotidesConceptsGene expressionAltered helical structureTarget sequenceSequence-specific mannerGenome modificationGenomic modificationsRepair pathwaysDNA-reactive compoundsOligonucleotide bindsDuplex DNAMajor grooveSpecific sitesHelical structureDual roleExpressionPossible roleCancer therapySequenceTFOGenomeTriplexesPolypyrimidineNumber of studiesDNABindsDistance and Affinity Dependence of Triplex-Induced Recombination †
Knauert MP, Lloyd JA, Rogers FA, Datta HJ, Bennett ML, Weeks DL, Glazer PM. Distance and Affinity Dependence of Triplex-Induced Recombination †. Biochemistry 2005, 44: 3856-3864. PMID: 15751961, DOI: 10.1021/bi0481040.Peer-Reviewed Original Research
2004
Peptide conjugates for chromosomal gene targeting by triplex-forming oligonucleotides
Rogers FA, Manoharan M, Rabinovitch P, Ward DC, Glazer PM. Peptide conjugates for chromosomal gene targeting by triplex-forming oligonucleotides. Nucleic Acids Research 2004, 32: 6595-6604. PMID: 15602001, PMCID: PMC545466, DOI: 10.1093/nar/gkh998.Peer-Reviewed Original ResearchConceptsMutation frequencyGene-targeted mutagenesisHomeodomain of AntennapediaDNA-binding moleculesChromosomal genesThird helixTarget genesChromosomal DNABiological activityAntpCell-penetrating peptidesGene expressionControl genesTransport peptideCellular uptakeAntigene compoundsGenesConfocal microscopyIntranuclear deliveryTFODose-responsive mannerHomeodomainAntennapediaOligonucleotideMutagenesis