Featured Publications
Enhancement of Impaired MRSA‐Infected Fracture Healing by Combinatorial Antibiotics and Modulation of Sustained Inflammation
Yu KE, Kwon H, Dussik CM, Cahill SV, Back J, Alder KD, Lee FY. Enhancement of Impaired MRSA‐Infected Fracture Healing by Combinatorial Antibiotics and Modulation of Sustained Inflammation. Journal Of Bone And Mineral Research 2020, 37: 1352-1365. PMID: 35616626, DOI: 10.1002/jbmr.4570.Peer-Reviewed Original ResearchConceptsMethicillin-resistant Staphylococcus aureusExcessive inflammationAntibiotic treatmentFracture healingTrametinib therapyInfected fracturesPro-inflammatory cytokine levelsPostoperative antibiotic administrationSetting of infectionMale C57BL/6 miceSystemic antibiotic treatmentAdjunctive therapeutic agentMRSA-infected miceImpaired callus formationFemoral fracture modelTherapeutic cohortCytokine levelsMRSA infectionMusculoskeletal infectionsPostoperative intervalsSystemic vancomycinAntibiotic administrationCytokine analysisC57BL/6 miceIntramedullary nailingLocally delivered adjuvant biofilm‐penetrating antibiotics rescue impaired endochondral fracture healing caused by MRSA infection
Cahill SV, Kwon H, Back J, Lee I, Lee S, Alder KD, Hao Z, Yu KE, Dussik CM, Kyriakides T, Lee F. Locally delivered adjuvant biofilm‐penetrating antibiotics rescue impaired endochondral fracture healing caused by MRSA infection. Journal Of Orthopaedic Research® 2021, 39: 402-414. PMID: 33336805, DOI: 10.1002/jor.24965.Peer-Reviewed Original ResearchConceptsHigh-dose rifampinFracture healingMRSA infectionMethicillin-resistant Staphylococcus aureus osteomyelitisColony-forming unitsTissue bacterial loadStaphylococcus aureus osteomyelitisReactive bone formationRadiographic union scaleEndochondral fracture healingBacterial CFU countsPoor callus formationSoft callus formationTibia scoresRifampin therapyDevastating complicationMRSA colonizationClinical efficacyInfected fracturesTibial radiographsTreatment responseMurine modelIntramedullary pinOpen fracturesO stainSmoking Alters Inflammation and Skeletal Stem and Progenitor Cell Activity During Fracture Healing in Different Murine Strains
Hao Z, Li J, Li B, Alder KD, Cahill SV, Munger AM, Lee I, Kwon H, Back J, Xu S, Kang M, Lee FY. Smoking Alters Inflammation and Skeletal Stem and Progenitor Cell Activity During Fracture Healing in Different Murine Strains. Journal Of Bone And Mineral Research 2020, 36: 186-198. PMID: 32866293, PMCID: PMC9057220, DOI: 10.1002/jbmr.4175.Peer-Reviewed Original ResearchConceptsCigarette smoke exposureCigarette smokingFracture healingSmoke exposureMurine modelMolecular alterationsBALB/cJ miceBiomechanical testingPro-inflammatory mediatorsCytokine/chemokine analysisImpaired fracture healingMultiplex cytokine/chemokine analysisInitial inflammatory responseFracture healing capacityBALB/cJProgenitor cell activitySmoking miceTransverse femoral osteotomyFemoral osteotomyImmune cellsChemokine analysisInflammatory responseFracture hematomaHigh riskMurine strains
2024
Unique Spatial Transcriptomic Profiling of the Murine Femoral Fracture Callus: A Preliminary Report
Jiang W, Caruana D, Back J, Lee F. Unique Spatial Transcriptomic Profiling of the Murine Femoral Fracture Callus: A Preliminary Report. Cells 2024, 13: 522. PMID: 38534368, PMCID: PMC10969736, DOI: 10.3390/cells13060522.Peer-Reviewed Original ResearchConceptsMDA-MB-231Transcriptomic platformsGene expressionSpatially localized gene expressionMDA-MB-231 metastatic breast cancer cellsSpatial transcriptomics platformGenetic expression changesFracture healingMetastatic breast cancer cellsBreast cancer cellsImpaired fracture healingSingle-cell RNALocal gene expressionSpatial transcriptomic profilingFracture callus formationGenomic heterogeneitySequencing techniquesDisrupt bone homeostasisTranscriptome profilingIn situ hybridizationExpression changesSoft callusPathological fracturesWild-typeSpatial transcriptomics
2023
Parathyroid hormone therapy improves MRSA-infected fracture healing in a murine diabetic model
Kwon H, Cahill S, Yu K, Alder K, Dussik C, Jeong J, Back J, Lee F. Parathyroid hormone therapy improves MRSA-infected fracture healing in a murine diabetic model. Frontiers In Cellular And Infection Microbiology 2023, 13: 1230568. PMID: 37829606, PMCID: PMC10565816, DOI: 10.3389/fcimb.2023.1230568.Peer-Reviewed Original ResearchConceptsFracture healingTeriparatide treatmentType 2 diabetic mouse modelType 2 diabetes mellitus miceBone volume/total volumeMurine diabetic modelTotal mineral densityIntermittent parathyroid hormoneSystemic antibiotic therapyImpairs fracture healingPoor fracture healingAdjuvant therapeutic agentDiabetes mellitus miceDiabetic mouse modelBlood glucose levelsCombination of antibioticsRadiographic union scaleTibia fracture modelIPTH treatmentSeptic nonunionNondiabetic counterpartsT2DM miceHormone therapyAntibiotic therapyMRSA infection
2020
Systemic Parathyroid Hormone Enhances Fracture Healing in Multiple Murine Models of Type 2 Diabetes Mellitus
Alder K, White A, Chung Y, Lee I, Back J, Kwon H, Cahill S, Hao Z, Li L, Chen F, Lee S, Riedel M, Lee F. Systemic Parathyroid Hormone Enhances Fracture Healing in Multiple Murine Models of Type 2 Diabetes Mellitus. JBMR Plus 2020, 4: e10359. PMID: 32382692, PMCID: PMC7202418, DOI: 10.1002/jbm4.10359.Peer-Reviewed Original ResearchRisk of fractureFracture healingMurine modelType 2 diabetes mellitusPTH expressionEffects of hPTHIntermittent systemic administrationSuitable murine modelPrevalence of T2DMAge-related osteoporosisDiabetes mellitusPresent studySystemic administrationSkeletal fragilityMultisystemic diseaseT2DMOsteoporotic phenotypeBone qualityBone fracturesCalcium homeostasisMesenchymal stem cellsBone phenotypeHormone productionHPTHHealing
2002
Repair of Bone Allograft Fracture Using Bone Morphogenetic Protein-2
Lee FY, Storer S, Hazan EJ, Gebhardt MC, Mankin HJ. Repair of Bone Allograft Fracture Using Bone Morphogenetic Protein-2. Clinical Orthopaedics And Related Research® 2002, 397: 119-126. PMID: 11953604, DOI: 10.1097/00003086-200204000-00017.Peer-Reviewed Original ResearchConceptsAllograft fractureNormal fracture healingFracture healingProtein 2Long-term clinical dataExtensive tumour surgeryBone morphogenetic protein-2Major revision surgeryMorphogenetic protein-2Experimental groupOnly fibrosisRadiographic unionNew bone formationRevision surgeryAdditional ratsClinical dataTumor surgeryBone allograftAllograft boneControl groupBiologic regulatorsOsteotomy siteFracture repairRecombinant human bone morphogenetic protein-2Adequate function
1997
Regional and temporal changes in the acoustic properties of fracture callus in secondary bone healing
Harten R, Lee F, Zimmerman M, Hurowitz E, Arakal R, Behrens F. Regional and temporal changes in the acoustic properties of fracture callus in secondary bone healing. Journal Of Orthopaedic Research® 1997, 15: 570-576. PMID: 9379267, DOI: 10.1002/jor.1100150413.Peer-Reviewed Original ResearchConceptsScanning acoustic microscopeMechanical propertiesAcoustic microscopyAcoustic microscopeMaterial propertiesAcoustic impedance mapsConventional testing methodsAcoustic propertiesImpedanceSecondary bone healingImpedance mapsTesting methodsPropertiesAccurate determinationBone healingPopular choiceFracturesLinear relationshipFracture healingDevicesPolymethylmethacrylateMicroscopeMean impedanceMaterials