2022
A mechanism of origin licensing control through autoinhibition of S. cerevisiae ORC·DNA·Cdc6
Schmidt JM, Yang R, Kumar A, Hunker O, Seebacher J, Bleichert F. A mechanism of origin licensing control through autoinhibition of S. cerevisiae ORC·DNA·Cdc6. Nature Communications 2022, 13: 1059. PMID: 35217664, PMCID: PMC8881611, DOI: 10.1038/s41467-022-28695-w.Peer-Reviewed Original ResearchConceptsOrigin recognition complexS. cerevisiaeCyclin-dependent kinase phosphorylationMcm2-7 loadingN-terminal domainCryo-electron microscopyCDK phosphorylationRecognition complexDNA replicationReplication originsÅ resolutionKinase phosphorylationMechanism of originCdc6Coordinated actionCerevisiaePhosphorylationDNAInhibitory signalsStructural detailsSite regulationRecruitmentOrc6AssemblyCdt1
2018
Conformational control and DNA-binding mechanism of the metazoan origin recognition complex
Bleichert F, Leitner A, Aebersold R, Botchan MR, Berger JM. Conformational control and DNA-binding mechanism of the metazoan origin recognition complex. Proceedings Of The National Academy Of Sciences Of The United States Of America 2018, 115: e5906-e5915. PMID: 29899147, PMCID: PMC6042147, DOI: 10.1073/pnas.1806315115.Peer-Reviewed Original ResearchConceptsOrigin recognition complexRecognition complexAutoinhibited stateHeterohexameric origin recognition complexMetazoan origin recognition complexHuman origin recognition complexUnstructured N-terminal regionORC-DNA interactionDNA-binding channelMcm2-7 loadingNucleotide-dependent associationCryo-EM analysisDNA-binding mechanismN-terminal regionMinichromosome maintenance 2Replicative helicaseATPase domainAutoinhibited configurationContact DNAHelix foldCellular activitiesMaintenance 2Biochemical assaysDNACdc6