2000
Close Association of the N Terminus of Kv1.3 with the Pore Region*
Yao X, Liu W, Tian S, Rafi H, Segal A, Desir G. Close Association of the N Terminus of Kv1.3 with the Pore Region*. Journal Of Biological Chemistry 2000, 275: 10859-10863. PMID: 10753881, DOI: 10.1074/jbc.275.15.10859.Peer-Reviewed Original ResearchConceptsN-terminusPore regionSteady-state protein levelsLarge single-channel conductanceVoltage-gated potassium channelsWild-type channelsShaker proteinCertain amino acidsChannel assemblyWild typeChannel proteinsChannel functionAmino acidsSingle-channel conductancePore blockersSpeed of inactivationTerminusProtein levelsDomain leadPore selectivityPotassium channelsProteinType channelsKinetic propertiesChannel conductance
1999
Defective processing and expression of thiazide-sensitive Na-Cl cotransporter as a cause of Gitelman’s syndrome
Kunchaparty S, Palcso M, Berkman J, Velázquez H, Desir G, Bernstein P, Reilly R, Ellison D. Defective processing and expression of thiazide-sensitive Na-Cl cotransporter as a cause of Gitelman’s syndrome. American Journal Of Physiology 1999, 277: f643-f649. PMID: 10516289, DOI: 10.1152/ajprenal.1999.277.4.f643.Peer-Reviewed Original ResearchConceptsWild-type cloneTransport proteinsWild-type proteinWild-type geneUnglycosylated proteinProtein processingNa-Cl cotransporterUnglycosylated formEndoplasmic reticulumMutant clonesFunctional expressionDisease mutationsDefective processingXenopus oocytesProteinClonesThiazide-sensitive Na-Cl cotransporterSodium uptakeMutationsOocytesMembrane stainingAutosomal recessive disorderWestern blotThe T0 Domain of Rabbit KV1.3 Regulates Steady State Channel Protein Level
Segal A, Yao X, Desir G. The T0 Domain of Rabbit KV1.3 Regulates Steady State Channel Protein Level. Biochemical And Biophysical Research Communications 1999, 254: 54-64. PMID: 9920732, DOI: 10.1006/bbrc.1998.9801.Peer-Reviewed Original ResearchConceptsN-terminal regulatory regionVoltage-gated potassium channelsWild-type channelsRegulatory regionsPlasma membraneAmino terminusChannel assemblyChannel proteinsRecognition domainSingle-channel conductanceKv channelsChannel protein levelsProtein levelsProtein densityPotassium channelsOpen probabilityType channelsChannel conductanceKv1.3Fast inactivationDomainMembraneTerminusProteinInactivation
1995
Molecular characterization of voltage and cyclic nucleotide-gated potassium channels in kidney
Desir G. Molecular characterization of voltage and cyclic nucleotide-gated potassium channels in kidney. Kidney International 1995, 48: 1031-1035. PMID: 8569064, DOI: 10.1038/ki.1995.386.Peer-Reviewed Original ResearchConceptsRenal K channelsMolecular characterizationInitial molecular characterizationPotassium channelsMammalian cellsMembrane proteinsMolecular biologyCell membraneK channelsDiverse groupPhysiologic roleKinetic propertiesPotential physiologic rolePassive movementBiologyProteinPhysiologyKidneyMembraneCharacterizationCellsExtensive dataThe structure, regulation and pathophysiology of potassium channels
Desir G. The structure, regulation and pathophysiology of potassium channels. Current Opinion In Nephrology & Hypertension 1995, 4: 402-405. PMID: 8564442, DOI: 10.1097/00041552-199509000-00005.Peer-Reviewed Original Research
1991
Reconstitution and partial purification of an amiloride-sensitive, cation channel from rabbit kidney
Desir G. Reconstitution and partial purification of an amiloride-sensitive, cation channel from rabbit kidney. Biochimica Et Biophysica Acta 1991, 1067: 38-42. PMID: 1868102, DOI: 10.1016/0005-2736(91)90023-2.Peer-Reviewed Original ResearchConceptsBrush border membrane vesiclesCation channelsMembrane vesicle proteinsChannel activityAmiloride-sensitive cation channelVesicle proteinsBorder membrane vesiclesMembrane vesiclesRabbit brush border membrane vesiclesProteoliposomesNative brush border membranesBrush border membraneSensitive cation channelPotassium channelsProteinPartial purificationAcridine orangeRenal brush-border membrane vesiclesBorder membraneDiethylthiadicarbocyanine iodideReconstitutionRabbit kidney