1996
Genomic structure and regulation of Kcn1, a cGMP-gated potassium channel
Yao X, Liu Y, Tung F, Desir GV. Genomic structure and regulation of Kcn1, a cGMP-gated potassium channel. American Journal Of Physiology 1996, 271: f37-f41. PMID: 8760241, DOI: 10.1152/ajprenal.1996.271.1.f37.Peer-Reviewed Original ResearchConceptsCis-regulatory elementsPromoter regionEnhancer elementsNovel potassium channel geneMajor transcription initiation siteTypical TATA boxTranscription initiation sitePhorbol esterPotassium channel genesLuciferase reporter constructsPotassium channelsGenomic structurePorcine kidney cell lineDeletion analysisRabbit geneTATA boxAcid proteinGene transcriptionKidney cell lineReporter constructsChannel genesSequence analysisPrimer extensionInitiation siteNorthern blot
1993
Identification of a novel K-channel gene (KC22) that is highly expressed in distal tubule of rabbit kidney
Desir GV, Velazquez H. Identification of a novel K-channel gene (KC22) that is highly expressed in distal tubule of rabbit kidney. American Journal Of Physiology 1993, 264: f128-f133. PMID: 7679255, DOI: 10.1152/ajprenal.1993.264.1.f128.Peer-Reviewed Original ResearchConceptsPolymerase chain reactionRabbit kidneyKidney cortexWhole kidney cortexShaker gene familyDistal tubule cellsVoltage-gated K channelsRenal tissueDistal tubulesTubule cellsRibonuclease protectionPrimary culturesKidneyChain reactionExpression levelsK channelsCortexTubulesPartial-length sequencesAbundant isoformIsoformsKidney cDNAK channel genes
1992
Isolation of putative voltage-gated epithelial K-channel isoforms from rabbit kidney and LLC-PK1 cells
Desir GV, Hamlin HA, Puente E, Reilly RF, Hildebrandt F, Igarashi P. Isolation of putative voltage-gated epithelial K-channel isoforms from rabbit kidney and LLC-PK1 cells. American Journal Of Physiology 1992, 262: f151-f157. PMID: 1733291, DOI: 10.1152/ajprenal.1992.262.1.f151.Peer-Reviewed Original ResearchConceptsDeduced amino acid sequenceAmino acid sequenceAcid sequenceVoltage-gated K channelsRabbit genomic DNAPutative transmembrane segmentsShaker-like genesPutative voltage sensorShaker gene familyVoltage-gated potassium channelsGene familyShaker proteinRenal epithelial cellsTransmembrane segmentsSequence similarityRabbit cDNAEpithelial cell lineSouthern analysisProtein sequencesLLC-PK1 cellsDifferent genesGenomic DNAKidney cDNACDNAS4 segment