2001
The distal convoluted tubule of rabbit kidney does not express a functional sodium channel
Velázquez H, Silva T, Andújar E, Desir G, Ellison D, Greger R. The distal convoluted tubule of rabbit kidney does not express a functional sodium channel. American Journal Of Physiology. Renal Physiology 2001, 280: f530-f539. PMID: 11181416, DOI: 10.1152/ajprenal.2001.280.3.f530.Peer-Reviewed Original ResearchAnimalsBlotting, NorthernCalcium-Binding ProteinsCloning, MolecularEndosomal Sorting Complexes Required for TransportEpithelial Sodium ChannelsIn Vitro TechniquesKidneyKidney Tubules, DistalLigasesMolecular Sequence DataNedd4 Ubiquitin Protein LigasesNephronsPerfusionRabbitsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSodium ChannelsTissue DistributionUbiquitin-Protein Ligases
1998
Rabbit distal convoluted tubule coexpresses NaCl cotransporter and 11β-hydroxysteroid dehydrogenase II mRNA
Velázquez H, Náray-Fejes-Tóth A, Silva T, Andújar E, Reilly RF, Desir GV, Ellison DH. Rabbit distal convoluted tubule coexpresses NaCl cotransporter and 11β-hydroxysteroid dehydrogenase II mRNA. Kidney International 1998, 54: 464-472. PMID: 9690213, DOI: 10.1046/j.1523-1755.1998.00036.x.Peer-Reviewed Original ResearchConceptsProximal tubulesNaCl cotransporterNephron segmentsDissected nephron segmentsTarget tissuesAction of aldosteroneAldosterone target tissuesSame nephron segmentsPolymerase chain reaction techniqueReverse transcriptase/polymerase chain reaction techniqueChain reaction techniqueDietary NaClDistal nephronBeta-HSDCCD samplesMRNA expressionNaCl cotransportRecent evidenceSimilar extentAldosteroneCotransporterHigh ratePrincipal targetTubulesTissue
1993
Identification of a novel K-channel gene (KC22) that is highly expressed in distal tubule of rabbit kidney
Desir GV, Velazquez H. Identification of a novel K-channel gene (KC22) that is highly expressed in distal tubule of rabbit kidney. American Journal Of Physiology 1993, 264: f128-f133. PMID: 7679255, DOI: 10.1152/ajprenal.1993.264.1.f128.Peer-Reviewed Original ResearchConceptsPolymerase chain reactionRabbit kidneyKidney cortexWhole kidney cortexShaker gene familyDistal tubule cellsVoltage-gated K channelsRenal tissueDistal tubulesTubule cellsRibonuclease protectionPrimary culturesKidneyChain reactionExpression levelsK channelsCortexTubulesPartial-length sequencesAbundant isoformIsoformsKidney cDNAK channel genesImmunocytochemical characterization of the high-affinity thiazide diuretic receptor in rabbit renal cortex
Ellison DH, Biemesderfer D, Morrisey J, Lauring J, Desir GV. Immunocytochemical characterization of the high-affinity thiazide diuretic receptor in rabbit renal cortex. American Journal Of Physiology 1993, 264: f141-f148. PMID: 8430824, DOI: 10.1152/ajprenal.1993.264.1.f141.Peer-Reviewed Original ResearchConceptsThiazide diuretic receptorRenal distal tubulesDistal tubulesUrinary NaCl excretionRenal cortical membranesThick ascending limbRabbit kidney cortexApical membraneThiazide diureticsRenal cortexRabbit renal cortexCortical membranesProximal tubulesAscending limbSubpopulation of cellsNaCl excretionThiazide receptorKidney cortexIntense stainingWestern blotMonoclonal antibodiesRabbit kidneyImmunocytochemical characterizationPrincipal cellsReceptors