2001
Guinea Pig Fertilin Exhibits Restricted Lateral Mobility in Epididymal Sperm and Becomes Freely Diffusing during Capacitation
Cowan A, Koppel D, Vargas L, Hunnicutt G. Guinea Pig Fertilin Exhibits Restricted Lateral Mobility in Epididymal Sperm and Becomes Freely Diffusing during Capacitation. Developmental Biology 2001, 236: 502-509. PMID: 11476588, DOI: 10.1006/dbio.2001.0343.Peer-Reviewed Original ResearchConceptsSperm-egg interactionLateral mobilityPlasma membranePlasma membrane bindingHead plasma membraneEpididymal spermPopulation of cellsFertilinMembrane bindingFluorescence redistributionHead domainMembrane bilayerAcrosome-intact spermEpididymal passageAcrosome-reacted spermSpermAcrosome reactionCauda epididymisMembraneCellsInteractionAcrosomeCapacitationBindingPhotobleaching
1996
Sperm Surface Protein PH-20 is Bifunctional: One Activity is a Hyaluronidase and a Second, Distinct Activity is Required in Secondary Sperm-Zona Binding
Hunnicutt G, Primakoff P, Myles D. Sperm Surface Protein PH-20 is Bifunctional: One Activity is a Hyaluronidase and a Second, Distinct Activity is Required in Secondary Sperm-Zona Binding. Biology Of Reproduction 1996, 55: 80-86. PMID: 8793062, DOI: 10.1095/biolreprod55.1.80.Peer-Reviewed Original ResearchConceptsSperm-zona bindingMonoclonal antibodiesHyaluronidase activityHyaluronic acidZona pellucidaPH-20Secondary sperm-zona bindingSperm penetrationAcrosome-reacted spermCumulus cell layersHyaluronidase inhibitorsCell layerRepetitive bindingAntibodiesHyaluronidaseAcrosome-intact spermOocytesPellucidaPrevious studiesActivityStructural Relationship of Sperm Soluble Hyaluronidase to the Sperm Membrane Protein PH-201
Hunnicutt G, Mahan K, Lathrop W, Ramarao C, Myles D, Primakoff P. Structural Relationship of Sperm Soluble Hyaluronidase to the Sperm Membrane Protein PH-201. Biology Of Reproduction 1996, 54: 1343-1349. PMID: 8724363, DOI: 10.1095/biolreprod54.6.1343.Peer-Reviewed Original ResearchConceptsSoluble protein fractionPH-20Soluble hyaluronidaseGlycosyl-phosphatidyl inositol (GPI) anchorProtein fractionsMembrane-bound endoproteaseAcrosome reactionPhosphatidyl inositol anchorMembrane anchorInner acrosomal membraneMembrane proteinsAcrosome-intact spermInositol anchorEndoproteolytic cleavageDisulfide-linked fragmentsGuinea pig spermMolecular massAcrosomal membraneEnzymatic activityPig spermAffinity columnCumulus cell layersSoluble formProteinEnzymatic release