Expression and Localization of Caveolin-1, and the Presence of Membrane Rafts, in Mouse and Guinea Pig Spermatozoa
Travis A, Merdiushev T, Vargas L, Jones B, Purdon M, Nipper R, Galatioto J, Moss S, Hunnicutt G, Kopf G. Expression and Localization of Caveolin-1, and the Presence of Membrane Rafts, in Mouse and Guinea Pig Spermatozoa. Developmental Biology 2001, 240: 599-610. PMID: 11784086, DOI: 10.1006/dbio.2001.0475.Peer-Reviewed Original ResearchConceptsMouse male germ cellsMale germ cellsCaveolin-1Membrane raftsDetergent-insoluble lipid raftsGerm cellsSimilar cellular functionsTrafficking of cholesterolImmunofluorescence analysisConfocal immunofluorescence analysisLight buoyant densityMembrane subdomainsGuinea pig spermatozoaSyntaxin 2Cellular functionsSucrose density gradient centrifugationFlagellar motilityLipid raftsIndirect immunofluorescence analysisNonraft fractionsSomatic cellsMembrane dynamicsAcrosomal biogenesisPig spermatozoaMature spermGuinea Pig Fertilin Exhibits Restricted Lateral Mobility in Epididymal Sperm and Becomes Freely Diffusing during Capacitation
Cowan A, Koppel D, Vargas L, Hunnicutt G. Guinea Pig Fertilin Exhibits Restricted Lateral Mobility in Epididymal Sperm and Becomes Freely Diffusing during Capacitation. Developmental Biology 2001, 236: 502-509. PMID: 11476588, DOI: 10.1006/dbio.2001.0343.Peer-Reviewed Original ResearchConceptsSperm-egg interactionLateral mobilityPlasma membranePlasma membrane bindingHead plasma membraneEpididymal spermPopulation of cellsFertilinMembrane bindingFluorescence redistributionHead domainMembrane bilayerAcrosome-intact spermEpididymal passageAcrosome-reacted spermSpermAcrosome reactionCauda epididymisMembraneCellsInteractionAcrosomeCapacitationBindingPhotobleaching