2001
Expression and Localization of Caveolin-1, and the Presence of Membrane Rafts, in Mouse and Guinea Pig Spermatozoa
Travis A, Merdiushev T, Vargas L, Jones B, Purdon M, Nipper R, Galatioto J, Moss S, Hunnicutt G, Kopf G. Expression and Localization of Caveolin-1, and the Presence of Membrane Rafts, in Mouse and Guinea Pig Spermatozoa. Developmental Biology 2001, 240: 599-610. PMID: 11784086, DOI: 10.1006/dbio.2001.0475.Peer-Reviewed Original ResearchConceptsMouse male germ cellsMale germ cellsCaveolin-1Membrane raftsDetergent-insoluble lipid raftsGerm cellsSimilar cellular functionsTrafficking of cholesterolImmunofluorescence analysisConfocal immunofluorescence analysisLight buoyant densityMembrane subdomainsGuinea pig spermatozoaSyntaxin 2Cellular functionsSucrose density gradient centrifugationFlagellar motilityLipid raftsIndirect immunofluorescence analysisNonraft fractionsSomatic cellsMembrane dynamicsAcrosomal biogenesisPig spermatozoaMature sperm
1997
Analysis of the Process of Localization of Fertilin to the Sperm Posterior Head Plasma Membrane Domain during Sperm Maturation in the Epididymis
Hunnicutt G, Koppel D, Myles D. Analysis of the Process of Localization of Fertilin to the Sperm Posterior Head Plasma Membrane Domain during Sperm Maturation in the Epididymis. Developmental Biology 1997, 191: 146-159. PMID: 9356178, DOI: 10.1006/dbio.1997.8700.Peer-Reviewed Original ResearchConceptsMembrane domainsSurface proteinsPlasma membraneHead domainSperm plasma membrane proteinsADAM protein familyPlasma membrane proteinsPlasma membrane domainsEgg plasma membraneSperm-egg fusionEntire sperm headPosterior headSperm surface moleculesFertilin betaProtein familyProtein localizationMembrane proteinsMetalloprotease domainFertilinDisintegrin domainLocalization patternsMature formSperm maturationProteolytic processingProtein