2005
The Sept4 Septin Locus Is Required for Sperm Terminal Differentiation in Mice
Kissel H, Georgescu M, Larisch S, Manova K, Hunnicutt G, Steller H. The Sept4 Septin Locus Is Required for Sperm Terminal Differentiation in Mice. Developmental Cell 2005, 8: 353-364. PMID: 15737931, DOI: 10.1016/j.devcel.2005.01.021.Peer-Reviewed Original ResearchConceptsCaspase inhibitor XIAPDiverse cellular functionsDifferent cellular compartmentsInhibition of XIAPGenomic lociMutant spermCellular functionsMouse developmentMitochondrial architectureCellular compartmentsTumor suppressionTerminal differentiationSperm maturationDefective spermRing-like structureTargeted deletionResidual cytoplasmProteinMutant miceXIAPLociTail regionCytoplasmSpermCytokinesis
2001
Expression and Localization of Caveolin-1, and the Presence of Membrane Rafts, in Mouse and Guinea Pig Spermatozoa
Travis A, Merdiushev T, Vargas L, Jones B, Purdon M, Nipper R, Galatioto J, Moss S, Hunnicutt G, Kopf G. Expression and Localization of Caveolin-1, and the Presence of Membrane Rafts, in Mouse and Guinea Pig Spermatozoa. Developmental Biology 2001, 240: 599-610. PMID: 11784086, DOI: 10.1006/dbio.2001.0475.Peer-Reviewed Original ResearchConceptsMouse male germ cellsMale germ cellsCaveolin-1Membrane raftsDetergent-insoluble lipid raftsGerm cellsSimilar cellular functionsTrafficking of cholesterolImmunofluorescence analysisConfocal immunofluorescence analysisLight buoyant densityMembrane subdomainsGuinea pig spermatozoaSyntaxin 2Cellular functionsSucrose density gradient centrifugationFlagellar motilityLipid raftsIndirect immunofluorescence analysisNonraft fractionsSomatic cellsMembrane dynamicsAcrosomal biogenesisPig spermatozoaMature spermGuinea Pig Fertilin Exhibits Restricted Lateral Mobility in Epididymal Sperm and Becomes Freely Diffusing during Capacitation
Cowan A, Koppel D, Vargas L, Hunnicutt G. Guinea Pig Fertilin Exhibits Restricted Lateral Mobility in Epididymal Sperm and Becomes Freely Diffusing during Capacitation. Developmental Biology 2001, 236: 502-509. PMID: 11476588, DOI: 10.1006/dbio.2001.0343.Peer-Reviewed Original ResearchConceptsSperm-egg interactionLateral mobilityPlasma membranePlasma membrane bindingHead plasma membraneEpididymal spermPopulation of cellsFertilinMembrane bindingFluorescence redistributionHead domainMembrane bilayerAcrosome-intact spermEpididymal passageAcrosome-reacted spermSpermAcrosome reactionCauda epididymisMembraneCellsInteractionAcrosomeCapacitationBindingPhotobleaching
1996
Sperm Surface Protein PH-20 is Bifunctional: One Activity is a Hyaluronidase and a Second, Distinct Activity is Required in Secondary Sperm-Zona Binding
Hunnicutt G, Primakoff P, Myles D. Sperm Surface Protein PH-20 is Bifunctional: One Activity is a Hyaluronidase and a Second, Distinct Activity is Required in Secondary Sperm-Zona Binding. Biology Of Reproduction 1996, 55: 80-86. PMID: 8793062, DOI: 10.1095/biolreprod55.1.80.Peer-Reviewed Original ResearchConceptsSperm-zona bindingMonoclonal antibodiesHyaluronidase activityHyaluronic acidZona pellucidaPH-20Secondary sperm-zona bindingSperm penetrationAcrosome-reacted spermCumulus cell layersHyaluronidase inhibitorsCell layerRepetitive bindingAntibodiesHyaluronidaseAcrosome-intact spermOocytesPellucidaPrevious studiesActivityStructural Relationship of Sperm Soluble Hyaluronidase to the Sperm Membrane Protein PH-201
Hunnicutt G, Mahan K, Lathrop W, Ramarao C, Myles D, Primakoff P. Structural Relationship of Sperm Soluble Hyaluronidase to the Sperm Membrane Protein PH-201. Biology Of Reproduction 1996, 54: 1343-1349. PMID: 8724363, DOI: 10.1095/biolreprod54.6.1343.Peer-Reviewed Original ResearchConceptsSoluble protein fractionPH-20Soluble hyaluronidaseGlycosyl-phosphatidyl inositol (GPI) anchorProtein fractionsMembrane-bound endoproteaseAcrosome reactionPhosphatidyl inositol anchorMembrane anchorInner acrosomal membraneMembrane proteinsAcrosome-intact spermInositol anchorEndoproteolytic cleavageDisulfide-linked fragmentsGuinea pig spermMolecular massAcrosomal membraneEnzymatic activityPig spermAffinity columnCumulus cell layersSoluble formProteinEnzymatic release