2001
Expression and Localization of Caveolin-1, and the Presence of Membrane Rafts, in Mouse and Guinea Pig Spermatozoa
Travis A, Merdiushev T, Vargas L, Jones B, Purdon M, Nipper R, Galatioto J, Moss S, Hunnicutt G, Kopf G. Expression and Localization of Caveolin-1, and the Presence of Membrane Rafts, in Mouse and Guinea Pig Spermatozoa. Developmental Biology 2001, 240: 599-610. PMID: 11784086, DOI: 10.1006/dbio.2001.0475.Peer-Reviewed Original ResearchConceptsMouse male germ cellsMale germ cellsCaveolin-1Membrane raftsDetergent-insoluble lipid raftsGerm cellsSimilar cellular functionsTrafficking of cholesterolImmunofluorescence analysisConfocal immunofluorescence analysisLight buoyant densityMembrane subdomainsGuinea pig spermatozoaSyntaxin 2Cellular functionsSucrose density gradient centrifugationFlagellar motilityLipid raftsIndirect immunofluorescence analysisNonraft fractionsSomatic cellsMembrane dynamicsAcrosomal biogenesisPig spermatozoaMature sperm
1996
Structural Relationship of Sperm Soluble Hyaluronidase to the Sperm Membrane Protein PH-201
Hunnicutt G, Mahan K, Lathrop W, Ramarao C, Myles D, Primakoff P. Structural Relationship of Sperm Soluble Hyaluronidase to the Sperm Membrane Protein PH-201. Biology Of Reproduction 1996, 54: 1343-1349. PMID: 8724363, DOI: 10.1095/biolreprod54.6.1343.Peer-Reviewed Original ResearchConceptsSoluble protein fractionPH-20Soluble hyaluronidaseGlycosyl-phosphatidyl inositol (GPI) anchorProtein fractionsMembrane-bound endoproteaseAcrosome reactionPhosphatidyl inositol anchorMembrane anchorInner acrosomal membraneMembrane proteinsAcrosome-intact spermInositol anchorEndoproteolytic cleavageDisulfide-linked fragmentsGuinea pig spermMolecular massAcrosomal membraneEnzymatic activityPig spermAffinity columnCumulus cell layersSoluble formProteinEnzymatic release