Expression and Localization of Caveolin-1, and the Presence of Membrane Rafts, in Mouse and Guinea Pig Spermatozoa
Travis A, Merdiushev T, Vargas L, Jones B, Purdon M, Nipper R, Galatioto J, Moss S, Hunnicutt G, Kopf G. Expression and Localization of Caveolin-1, and the Presence of Membrane Rafts, in Mouse and Guinea Pig Spermatozoa. Developmental Biology 2001, 240: 599-610. PMID: 11784086, DOI: 10.1006/dbio.2001.0475.Peer-Reviewed Original ResearchMeSH KeywordsAcrosomeAnimalsCaveolin 1CaveolinsFluorescent Antibody Technique, IndirectGuinea PigsImmunoblottingMaleMembrane MicrodomainsMiceMicroscopy, ConfocalSignal TransductionSperm CapacitationSperm MotilitySpermatogenesisSpermatozoaConceptsMouse male germ cellsMale germ cellsCaveolin-1Membrane raftsDetergent-insoluble lipid raftsGerm cellsSimilar cellular functionsTrafficking of cholesterolImmunofluorescence analysisConfocal immunofluorescence analysisLight buoyant densityMembrane subdomainsGuinea pig spermatozoaSyntaxin 2Cellular functionsSucrose density gradient centrifugationFlagellar motilityLipid raftsIndirect immunofluorescence analysisNonraft fractionsSomatic cellsMembrane dynamicsAcrosomal biogenesisPig spermatozoaMature sperm