Sperm Surface Protein PH-20 is Bifunctional: One Activity is a Hyaluronidase and a Second, Distinct Activity is Required in Secondary Sperm-Zona Binding
Hunnicutt G, Primakoff P, Myles D. Sperm Surface Protein PH-20 is Bifunctional: One Activity is a Hyaluronidase and a Second, Distinct Activity is Required in Secondary Sperm-Zona Binding. Biology Of Reproduction 1996, 55: 80-86. PMID: 8793062, DOI: 10.1095/biolreprod55.1.80.Peer-Reviewed Original ResearchConceptsSperm-zona bindingMonoclonal antibodiesHyaluronidase activityHyaluronic acidZona pellucidaPH-20Secondary sperm-zona bindingSperm penetrationAcrosome-reacted spermCumulus cell layersHyaluronidase inhibitorsCell layerRepetitive bindingAntibodiesHyaluronidaseAcrosome-intact spermOocytesPellucidaPrevious studiesActivity