Sperm Surface Protein PH-20 is Bifunctional: One Activity is a Hyaluronidase and a Second, Distinct Activity is Required in Secondary Sperm-Zona Binding
Hunnicutt G, Primakoff P, Myles D. Sperm Surface Protein PH-20 is Bifunctional: One Activity is a Hyaluronidase and a Second, Distinct Activity is Required in Secondary Sperm-Zona Binding. Biology Of Reproduction 1996, 55: 80-86. PMID: 8793062, DOI: 10.1095/biolreprod55.1.80.Peer-Reviewed Original ResearchConceptsSperm-zona bindingMonoclonal antibodiesHyaluronidase activityHyaluronic acidZona pellucidaPH-20Secondary sperm-zona bindingSperm penetrationAcrosome-reacted spermCumulus cell layersHyaluronidase inhibitorsCell layerRepetitive bindingAntibodiesHyaluronidaseAcrosome-intact spermOocytesPellucidaPrevious studiesActivityStructural Relationship of Sperm Soluble Hyaluronidase to the Sperm Membrane Protein PH-201
Hunnicutt G, Mahan K, Lathrop W, Ramarao C, Myles D, Primakoff P. Structural Relationship of Sperm Soluble Hyaluronidase to the Sperm Membrane Protein PH-201. Biology Of Reproduction 1996, 54: 1343-1349. PMID: 8724363, DOI: 10.1095/biolreprod54.6.1343.Peer-Reviewed Original ResearchConceptsSoluble protein fractionPH-20Soluble hyaluronidaseGlycosyl-phosphatidyl inositol (GPI) anchorProtein fractionsMembrane-bound endoproteaseAcrosome reactionPhosphatidyl inositol anchorMembrane anchorInner acrosomal membraneMembrane proteinsAcrosome-intact spermInositol anchorEndoproteolytic cleavageDisulfide-linked fragmentsGuinea pig spermMolecular massAcrosomal membraneEnzymatic activityPig spermAffinity columnCumulus cell layersSoluble formProteinEnzymatic release