2012
Trainee Forum—Writing Winning Grants.
Taymans S, Moss S, Hunnicutt G. Trainee Forum—Writing Winning Grants. Biology Of Reproduction 2012, 87: 1-1. DOI: 10.1093/biolreprod/87.s1.1.Peer-Reviewed Original Research
2007
DIRECT EVIDENCE THAT THE SPERM ANNULUS IS REQUIRED FOR MEMBRANE DOMAIN MAINTENANCE AND NORMAL SPERM MATURATION
Hunnicutt G, Fernandez M, Steller H, Kwitny S. DIRECT EVIDENCE THAT THE SPERM ANNULUS IS REQUIRED FOR MEMBRANE DOMAIN MAINTENANCE AND NORMAL SPERM MATURATION. Biology Of Reproduction 2007, 77: 115-115. DOI: 10.1093/biolreprod/77.s1.115.Peer-Reviewed Original ResearchGM1 DYNAMICS INDICATE MEMBRANE CHANGES ASSOCIATED WITH CAPACITATION IN MURINE SPERMATOZOA
Selvaraj V, Buttke D, Atsushi A, Nelson J, Klaus A, Hunnicutt G, Travis A. GM1 DYNAMICS INDICATE MEMBRANE CHANGES ASSOCIATED WITH CAPACITATION IN MURINE SPERMATOZOA. Biology Of Reproduction 2007, 77: 166-166. DOI: 10.1093/biolreprod/77.s1.166.Peer-Reviewed Original ResearchPost-acrosomal plasma membranePlasma membraneAcrosomal exocytosisSterol effluxProgesterone-induced acrosomal exocytosisMurine spermatozoaMembrane changesMurine spermDramatic redistributionLive cellsBovine sperm headSperm deathSubunit BExocytosisStimulus-specific changesMale fertilitySperm headCapacitationSperm responseStimulus-specific patternsCholera toxin subunit BMembraneGanglioside GM1Sperm populationSpecific stimuli171 Creation of a SCCRO (DCN-1) knockout mouse results in decreased CUL3 neddylation and male infertility
Kaufman A, Kim A, Ryan R, Huryn L, Conway A, Manova K, Morris P, Hunnicutt G, Ramanathan Y, Singh B. 171 Creation of a SCCRO (DCN-1) knockout mouse results in decreased CUL3 neddylation and male infertility. Journal Of Surgical Research 2007, 137: 224. DOI: 10.1016/j.jss.2006.12.186.Peer-Reviewed Original Research
2006
Ultrastructural Features of Mammalian Sperm: Applications of Cold Field-Emission Scanning Electron Microscopy
Klaus A, Hunnicutt G. Ultrastructural Features of Mammalian Sperm: Applications of Cold Field-Emission Scanning Electron Microscopy. Microscopy And Microanalysis 2006, 12: 232-233. DOI: 10.1017/s1431927606067614.Peer-Reviewed Original Research
2005
Immune Complex Deposition in Adult Male Sprague–Dawley Rats Chronically Immunized with GnRH
Vargas L, Sewell R, Marshall A, Galatioto J, Tsong Y, Catterall J, Hunnicutt G. Immune Complex Deposition in Adult Male Sprague–Dawley Rats Chronically Immunized with GnRH. American Journal Of Reproductive Immunology 2005, 54: 292-310. PMID: 16212651, DOI: 10.1111/j.1600-0897.2005.00310.x.Peer-Reviewed Original ResearchConceptsImmune complex depositionImmune complexesComplex depositionChronic immunizationAdult male Sprague-Dawley ratsMale Sprague-Dawley ratsAnti-GnRH antibodiesGlomerular immune complexesPotential deleterious side effectsSprague-Dawley ratsDeleterious side effectsIC depositionPathologic effectsSide effectsEffective immunocontraceptiveImmunizationFinal analysisOrgan analysisCellular damageRatsMost tissuesImmunofluorescence microscopyCellular levelAnimalsGnRHThe Sept4 Septin Locus Is Required for Sperm Terminal Differentiation in Mice
Kissel H, Georgescu M, Larisch S, Manova K, Hunnicutt G, Steller H. The Sept4 Septin Locus Is Required for Sperm Terminal Differentiation in Mice. Developmental Cell 2005, 8: 353-364. PMID: 15737931, DOI: 10.1016/j.devcel.2005.01.021.Peer-Reviewed Original ResearchConceptsCaspase inhibitor XIAPDiverse cellular functionsDifferent cellular compartmentsInhibition of XIAPGenomic lociMutant spermCellular functionsMouse developmentMitochondrial architectureCellular compartmentsTumor suppressionTerminal differentiationSperm maturationDefective spermRing-like structureTargeted deletionResidual cytoplasmProteinMutant miceXIAPLociTail regionCytoplasmSpermCytokinesis
2001
Expression and Localization of Caveolin-1, and the Presence of Membrane Rafts, in Mouse and Guinea Pig Spermatozoa
Travis A, Merdiushev T, Vargas L, Jones B, Purdon M, Nipper R, Galatioto J, Moss S, Hunnicutt G, Kopf G. Expression and Localization of Caveolin-1, and the Presence of Membrane Rafts, in Mouse and Guinea Pig Spermatozoa. Developmental Biology 2001, 240: 599-610. PMID: 11784086, DOI: 10.1006/dbio.2001.0475.Peer-Reviewed Original ResearchConceptsMouse male germ cellsMale germ cellsCaveolin-1Membrane raftsDetergent-insoluble lipid raftsGerm cellsSimilar cellular functionsTrafficking of cholesterolImmunofluorescence analysisConfocal immunofluorescence analysisLight buoyant densityMembrane subdomainsGuinea pig spermatozoaSyntaxin 2Cellular functionsSucrose density gradient centrifugationFlagellar motilityLipid raftsIndirect immunofluorescence analysisNonraft fractionsSomatic cellsMembrane dynamicsAcrosomal biogenesisPig spermatozoaMature spermGuinea Pig Fertilin Exhibits Restricted Lateral Mobility in Epididymal Sperm and Becomes Freely Diffusing during Capacitation
Cowan A, Koppel D, Vargas L, Hunnicutt G. Guinea Pig Fertilin Exhibits Restricted Lateral Mobility in Epididymal Sperm and Becomes Freely Diffusing during Capacitation. Developmental Biology 2001, 236: 502-509. PMID: 11476588, DOI: 10.1006/dbio.2001.0343.Peer-Reviewed Original ResearchConceptsSperm-egg interactionLateral mobilityPlasma membranePlasma membrane bindingHead plasma membraneEpididymal spermPopulation of cellsFertilinMembrane bindingFluorescence redistributionHead domainMembrane bilayerAcrosome-intact spermEpididymal passageAcrosome-reacted spermSpermAcrosome reactionCauda epididymisMembraneCellsInteractionAcrosomeCapacitationBindingPhotobleaching
1998
Neural Crest-Specific and General Expression of Distinct Metalloprotease–Disintegrins in EarlyXenopus laevisDevelopment
Cai H, Krätzschmar J, Alfandari D, Hunnicutt G, Blobel C. Neural Crest-Specific and General Expression of Distinct Metalloprotease–Disintegrins in EarlyXenopus laevisDevelopment. Developmental Biology 1998, 204: 508-524. PMID: 9882486, DOI: 10.1006/dbio.1998.9017.Peer-Reviewed Original Research
1997
Analysis of the Process of Localization of Fertilin to the Sperm Posterior Head Plasma Membrane Domain during Sperm Maturation in the Epididymis
Hunnicutt G, Koppel D, Myles D. Analysis of the Process of Localization of Fertilin to the Sperm Posterior Head Plasma Membrane Domain during Sperm Maturation in the Epididymis. Developmental Biology 1997, 191: 146-159. PMID: 9356178, DOI: 10.1006/dbio.1997.8700.Peer-Reviewed Original ResearchConceptsMembrane domainsSurface proteinsPlasma membraneHead domainSperm plasma membrane proteinsADAM protein familyPlasma membrane proteinsPlasma membrane domainsEgg plasma membraneSperm-egg fusionEntire sperm headPosterior headSperm surface moleculesFertilin betaProtein familyProtein localizationMembrane proteinsMetalloprotease domainFertilinDisintegrin domainLocalization patternsMature formSperm maturationProteolytic processingProtein
1996
Structural Change of the Endoplasmic Reticulum during Fertilization: Evidence for Loss of Membrane Continuity Using the Green Fluorescent Protein
Terasaki M, Jaffe L, Hunnicutt G, Hammer J. Structural Change of the Endoplasmic Reticulum during Fertilization: Evidence for Loss of Membrane Continuity Using the Green Fluorescent Protein. Developmental Biology 1996, 179: 320-328. PMID: 8903348, DOI: 10.1006/dbio.1996.0263.Peer-Reviewed Original ResearchSperm Surface Protein PH-20 is Bifunctional: One Activity is a Hyaluronidase and a Second, Distinct Activity is Required in Secondary Sperm-Zona Binding
Hunnicutt G, Primakoff P, Myles D. Sperm Surface Protein PH-20 is Bifunctional: One Activity is a Hyaluronidase and a Second, Distinct Activity is Required in Secondary Sperm-Zona Binding. Biology Of Reproduction 1996, 55: 80-86. PMID: 8793062, DOI: 10.1095/biolreprod55.1.80.Peer-Reviewed Original ResearchConceptsSperm-zona bindingMonoclonal antibodiesHyaluronidase activityHyaluronic acidZona pellucidaPH-20Secondary sperm-zona bindingSperm penetrationAcrosome-reacted spermCumulus cell layersHyaluronidase inhibitorsCell layerRepetitive bindingAntibodiesHyaluronidaseAcrosome-intact spermOocytesPellucidaPrevious studiesActivityStructural Relationship of Sperm Soluble Hyaluronidase to the Sperm Membrane Protein PH-201
Hunnicutt G, Mahan K, Lathrop W, Ramarao C, Myles D, Primakoff P. Structural Relationship of Sperm Soluble Hyaluronidase to the Sperm Membrane Protein PH-201. Biology Of Reproduction 1996, 54: 1343-1349. PMID: 8724363, DOI: 10.1095/biolreprod54.6.1343.Peer-Reviewed Original ResearchConceptsSoluble protein fractionPH-20Soluble hyaluronidaseGlycosyl-phosphatidyl inositol (GPI) anchorProtein fractionsMembrane-bound endoproteaseAcrosome reactionPhosphatidyl inositol anchorMembrane anchorInner acrosomal membraneMembrane proteinsAcrosome-intact spermInositol anchorEndoproteolytic cleavageDisulfide-linked fragmentsGuinea pig spermMolecular massAcrosomal membraneEnzymatic activityPig spermAffinity columnCumulus cell layersSoluble formProteinEnzymatic release