Intra-Subtype Variation in Enteroadhesion Accounts for Differences in Epithelial Barrier Disruption and Is Associated with Metronidazole Resistance in Blastocystis Subtype-7
Wu Z, Mirza H, Tan KS. Intra-Subtype Variation in Enteroadhesion Accounts for Differences in Epithelial Barrier Disruption and Is Associated with Metronidazole Resistance in Blastocystis Subtype-7. PLOS Neglected Tropical Diseases 2014, 8: e2885. PMID: 24851944, PMCID: PMC4031124, DOI: 10.1371/journal.pntd.0002885.Peer-Reviewed Original ResearchConceptsEpithelial barrier disruptionBarrier disruptionDrug resistanceBlastocystis subtype 7Intra-subtype variationOccludin tight junction proteinsEpithelial barrier dysfunctionDiverse clinical outcomesParasite virulence factorsTight junction proteinsCaco-2 cellsClinical outcomesBarrier dysfunctionIntestinal disordersMetronidazole resistanceBlastocystis infectionSubtype 7Metronidazole susceptibilityHost responseIntestinal epitheliumEnteric pathogensNitrosative stressResistant strainsJunction proteinsLuminal stagesStrain‐Dependent Induction of Human Enterocyte Apoptosis by Blastocystis Disrupts Epithelial Barrier and ZO‐1 Organization in a Caspase 3‐ and 9‐Dependent Manner
Wu Z, Mirza H, Teo JD, Tan KS. Strain‐Dependent Induction of Human Enterocyte Apoptosis by Blastocystis Disrupts Epithelial Barrier and ZO‐1 Organization in a Caspase 3‐ and 9‐Dependent Manner. BioMed Research International 2014, 2014: 209163. PMID: 24822183, PMCID: PMC4009109, DOI: 10.1155/2014/209163.Peer-Reviewed Original ResearchConceptsEpithelial barrier compromiseEnterocyte apoptosisHuman colonic epithelial cell line Caco-2Barrier compromiseHuman intestinal epithelial barrier functionIntestinal epithelial barrier functionHuman intestineBlastocystis subtype 7Epithelial cell line Caco-2Caspase-3ST-7Epithelial barrier functionZO-1 organizationStrain-dependent inductionCell line Caco-2Caco-2 monolayersAbdominal painIntestinal symptomsPan-caspase inhibitor Z-VAD-FMKEpithelial permeabilitySubtype 7Subtype 4Epithelial barrierEpithelial resistanceBarrier function