2021
Tumor suppressor p53 regulates intestinal type 2 immunity
Chang C, Wang J, Zhao Y, Liu J, Yang X, Yue X, Wang H, Zhou F, Inclan-Rico J, Ponessa J, Xie P, Zhang L, Siracusa M, Feng Z, Hu W. Tumor suppressor p53 regulates intestinal type 2 immunity. Nature Communications 2021, 12: 3371. PMID: 34099671, PMCID: PMC8184793, DOI: 10.1038/s41467-021-23587-x.Peer-Reviewed Original ResearchConceptsType 2 immunityRole of p53Parasitic infectionsInnate lymphoid cell responsesLymphoid cell responsesImmune integrityInfection of parasitesNippostrongylus brasiliensisTuft cellsCell responsesImmunityInfectionTritrichomonas murisTumor suppressor p53P53Unrecognized functionTumor suppressionSuppressor p53Critical triggerCritical roleCell circuitRoleResponse
2020
Electronic Cigarettes Induce Mitochondrial DNA Damage and Trigger TLR9 (Toll-Like Receptor 9)-Mediated Atherosclerosis
Li J, Huynh L, Cornwell WD, Tang MS, Simborio H, Huang J, Kosmider B, Rogers TJ, Zhao H, Steinberg MB, Thu Thi Le L, Zhang L, Pham K, Liu C, Wang H. Electronic Cigarettes Induce Mitochondrial DNA Damage and Trigger TLR9 (Toll-Like Receptor 9)-Mediated Atherosclerosis. Arteriosclerosis Thrombosis And Vascular Biology 2020, 41: 839-853. PMID: 33380174, PMCID: PMC8608030, DOI: 10.1161/atvbaha.120.315556.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaAtherosclerosisDisease Models, AnimalDNA DamageDNA, MitochondrialE-Cigarette VaporFemaleHumansInflammationInflammation MediatorsMacrophagesMaleMiceMice, Inbred C57BLMice, Knockout, ApoEMiddle AgedMitochondriaRAW 264.7 CellsSignal TransductionSmokersToll-Like Receptor 9VapingConceptsECV exposureTLR9 expressionInflammatory cytokinesClassical monocytesTLR9 activationAtherosclerotic plaquesEight-week-old ApoEUpregulation of TLR9Expression of TLR9Atherosclerotic lesion developmentOil Red O stainingToll-like receptorsDays/weekE-cig exposureMonocytes/macrophagesNormal laboratory dietRed O stainingPotential pharmacological targetElectronic cigarette useHours/dayProinflammatory cytokinesCig vaporPlasma levelsTLR9 antagonistTLR9 inhibitorThe Role of Immunological Synapse in Predicting the Efficacy of Chimeric Antigen Receptor (CAR) Immunotherapy
Liu D, Badeti S, Dotti G, Jiang JG, Wang H, Dermody J, Soteropoulos P, Streck D, Birge RB, Liu C. The Role of Immunological Synapse in Predicting the Efficacy of Chimeric Antigen Receptor (CAR) Immunotherapy. Cell Communication And Signaling 2020, 18: 134. PMID: 32843053, PMCID: PMC7446110, DOI: 10.1186/s12964-020-00617-7.Peer-Reviewed Original ResearchConceptsChimeric antigen receptor (CAR) immunotherapyCAR immunotherapyCancer immunotherapyClinical outcomesImmune cellsImmunological synapseCancer therapyPrecision cancer immunotherapyRefractory blood cancersRecent clinical trialsTumor antigen specificityUnique cell surface receptorChimeric antigen receptorImmune cell activationAdoptive transferImmunotherapeutic strategiesSuppressor cellsClinical predictorsCell immunotherapyClinical trialsIndividual patientsImmunotherapyAntigen specificityCell activationBlood cancersE-cigarette promotes breast carcinoma progression and lung metastasis: Macrophage-tumor cells crosstalk and the role of CCL5 and VCAM-1
Pham K, Huynh D, Le L, Delitto D, Yang L, Huang J, Kang Y, Steinberg MB, Li J, Zhang L, Liu D, Tang MS, Liu C, Wang H. E-cigarette promotes breast carcinoma progression and lung metastasis: Macrophage-tumor cells crosstalk and the role of CCL5 and VCAM-1. Cancer Letters 2020, 491: 132-145. PMID: 32829009, PMCID: PMC9703643, DOI: 10.1016/j.canlet.2020.08.010.Peer-Reviewed Original ResearchConceptsBC cell growthCig exposureLung metastasesBreast cancerVCAM-1V-CAM-1Role of CCL5Upregulated protein expressionBC cell survivalE-cig exposurePro-tumorigenic factorsBC cell apoptosisBreast carcinoma progressionMetastatic lung colonizationCCR5 axisMFP tumorsTAMs infiltrationInfiltrated macrophagesCell growthCo-culture systemImmunohistochemical stainsCell crosstalkBC cellsBC growthProliferation indexLIF is essential for ISC function and protects against radiation-induced gastrointestinal syndrome
Wang H, Wang J, Zhao Y, Zhang X, Liu J, Zhang C, Haffty B, Verzi M, Zhang L, Gao N, Feng Z, Hu W. LIF is essential for ISC function and protects against radiation-induced gastrointestinal syndrome. Cell Death & Disease 2020, 11: 588. PMID: 32719388, PMCID: PMC7385639, DOI: 10.1038/s41419-020-02790-6.Peer-Reviewed Original ResearchConceptsRadiation-induced GI syndromeLeukemia inhibitory factorIntestinal epitheliumIntestinal stem cellsLIF deficiencyGI syndromeGastrointestinal syndromeFunction of ISCsRadiation-induced gastrointestinal syndromeRecombinant leukemia inhibitory factorRecombinant LIFWild-type miceISC functionLifespan of miceAkt/GSK3βIntestinal epithelial regenerationIntestinal epithelial homeostasisStem cellsProlong survivalWT miceAdult intestinal epitheliumRadioprotective roleΒ-catenin activityCytokine essentialHigh dosesThe role of survivin in the progression of pancreatic ductal adenocarcinoma (PDAC) and a novel survivin-targeted therapeutic for PDAC
Brown M, Zhang W, Yan D, Kenath R, Le L, Wang H, Delitto D, Ostrov D, Robertson K, Liu C, Pham K. The role of survivin in the progression of pancreatic ductal adenocarcinoma (PDAC) and a novel survivin-targeted therapeutic for PDAC. PLOS ONE 2020, 15: e0226917. PMID: 31929540, PMCID: PMC6957139, DOI: 10.1371/journal.pone.0226917.Peer-Reviewed Original ResearchConceptsPancreatic ductal adenocarcinomaTypes of cancerDuctal adenocarcinomaSurvivin expressionSurvivin inhibitorClinical response rateNovel survivin inhibitorHalf of patientsElevated survivin expressionLower patient survivalPancreatic tumor microenvironmentPotential therapeutic targetExpression of survivinRole of survivinField of oncologyPancreatic cancer linesImmunotherapeutic approachesPatient survivalUntreated cohortTherapeutic responseInhibitor of survivinTreatment resistancePDAC progressionEffective treatmentTumor cell migration
2016
Anterior Leaflet Augmentation With CorMatrix Porcine Extracellular Matrix in Twenty-Five Patients: Unexpected Patch Failures and Histologic Analysis
Kelley T, Kashem M, Wang H, McCarthy J, Carroll N, Moser G, Guy T. Anterior Leaflet Augmentation With CorMatrix Porcine Extracellular Matrix in Twenty-Five Patients: Unexpected Patch Failures and Histologic Analysis. The Annals Of Thoracic Surgery 2016, 103: 114-120. PMID: 27623276, DOI: 10.1016/j.athoracsur.2016.05.090.Peer-Reviewed Original ResearchConceptsAnterior leaflet augmentationSevere mitral regurgitationMitral regurgitationLeaflet augmentationLate deathsAutologous pericardiumECM patchPorcine extracellular matrixType of MRChronic obstructive pulmonary diseaseLower body mass indexFollow-up echocardiogramChronic renal failureObstructive pulmonary diseaseSubset of patientsVentricular ejection fractionBody mass indexSuture line dehiscenceResult of complicationsCorMatrix Extracellular MatrixTemple University HospitalDa Vinci surgical robotExtracellular matrixCause 7Chart review
2013
Altered vascular activation due to deficiency of the NADPH oxidase component p22phox
Wang H, Albadawi H, Siddiquee Z, Stone J, Panchenko M, Watkins M, Stone J. Altered vascular activation due to deficiency of the NADPH oxidase component p22phox. Cardiovascular Pathology 2013, 23: 35-42. PMID: 24035466, DOI: 10.1016/j.carpath.2013.08.003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarotid Artery InjuriesCarotid Artery, CommonCase-Control StudiesCasein Kinase IalphaCells, CulturedCoronary VesselsCytochrome b GroupElastic TissueFemaleGranulomatous Disease, ChronicHeterogeneous-Nuclear Ribonucleoprotein Group CHumansHyperplasiaInfantMaleMatrix Metalloproteinase 12MiceMice, KnockoutMuscle, Smooth, VascularNADPH OxidasesNeointimaReactive Oxygen SpeciesRNA InterferenceTissue Inhibitor of Metalloproteinase-1TransfectionConceptsWild-type littermatesElastic fiber lossVascular activationDeficient miceIntimal hyperplasiaNADPH oxidase complexCoronary artery smooth muscle cellsNicotinamide adenine dinucleotide phosphate (NADPH) oxidaseArtery smooth muscle cellsHuman coronary artery smooth muscle cellsAdenine Dinucleotide Phosphate OxidaseBasal blood pressureCarotid artery ligationExpression ratioSmooth muscle cellsLung biopsyBlood pressureFiber lossArtery ligationCarotid ligationCarotid arteryTissue inhibitorHuman patientsMuscle cellsMiceSEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion
Chen X, Wang H, Bajaj K, Zhang P, Meng Z, Ma D, Bai Y, Liu H, Adams E, Baines A, Yu G, Sartor M, Zhang B, Yi Z, Lin J, Young S, Schekman R, Ginsburg D. SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion. ELife 2013, 2: e00444. PMID: 23580231, PMCID: PMC3622177, DOI: 10.7554/elife.00444.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApolipoproteins ECell Line, TumorCholesterolCOP-Coated VesiclesDown-RegulationDyslipidemiasEndoplasmic ReticulumEpistasis, GeneticGenotypeHEK293 CellsHumansLiverMice, 129 StrainMice, Inbred C57BLMice, KnockoutMutationPhenotypeProprotein Convertase 9Proprotein ConvertasesProtein TransportReceptors, LDLSerine EndopeptidasesTransfectionVesicular Transport ProteinsConceptsEndoplasmic reticulumCOPII vesicle formationCargo recruitmentCOPII vesiclesCargo proteinsEukaryotic cellsCargo selectivitySecretory pathwaySEC24AEfficient exitUnappreciated heterogeneityNegative regulatorSecretory proteinsVesicle formationPCSK9 secretionComplete genetic deficiencyGenetic deficiencyLDLR levelsProteinVesiclesFundamental roleNormal survivalPartial overlapRecruitmentSEC24B
2012
SEC23B is required for the maintenance of murine professional secretory tissues
Tao J, Zhu M, Wang H, Afelik S, Vasievich M, Chen X, Zhu G, Jensen J, Ginsburg D, Zhang B. SEC23B is required for the maintenance of murine professional secretory tissues. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: e2001-e2009. PMID: 22745161, PMCID: PMC3406820, DOI: 10.1073/pnas.1209207109.Peer-Reviewed Original ResearchMeSH KeywordsAlcian BlueAnimalsAnthraquinonesApoptosisEndoplasmic ReticulumFluorescent Antibody TechniqueHumansIn Situ Nick-End LabelingMiceMice, Inbred C57BLMice, TransgenicMicroscopy, ImmunoelectronMutationPancreasReal-Time Polymerase Chain ReactionSecretory PathwaySpecies SpecificityVesicular Transport ProteinsConceptsEndoplasmic reticulumSecretory tissueSpecies-specific shiftsGTPase-activating proteinsUnfolded protein responseAccumulation of proteinsSimilar ultrastructural alterationsAbundant cargoCargo recognitionEukaryotic cellsSar1 GTPaseER exitER lumenSecretory pathwayTissue-specific dependenceProtein responseHypomorphic mutationsSecretory proteinsSEC23BDisparate phenotypesCOPIICongenital dyserythropoietic anemia type IIEmbryonic pancreasAnemia phenotypeProapoptotic pathways
2008
Transgenic overexpression of a stable Plasminogen Activator Inhibitor-1 variant
Fahim A, Wang H, Feng J, Ginsburg D. Transgenic overexpression of a stable Plasminogen Activator Inhibitor-1 variant. Thrombosis Research 2008, 123: 785-792. PMID: 18774162, PMCID: PMC2670886, DOI: 10.1016/j.thromres.2008.07.004.Peer-Reviewed Original ResearchConceptsPlasminogen activator inhibitor-1Plasma PAI-1 levelsPAI-1 levelsPAI-1 mRNAHigher plasma PAI-1 levelsTransgenic overexpressionEvidence of thrombosisActivator inhibitor-1PAI-1 variantsApparent thrombosisHistologic examinationSerine protease inhibitor gene familyPhysiologic effectsTransgenic miceChicken beta-actin promoterModerate expressionInhibitor-1High expressionBeta-actin promoterBiologic importanceThrombosisPhysiologic conditionsKidneyMiceLiver
2004
Dose‐Dependent Neovascularization‐Promoting Effect of Adenovirus Vector CI‐1023 in a Rat Hindlimb Ischaemic Model
Wang H, Keiser J, Olszewski B, Brammer D, Gordon D. Dose‐Dependent Neovascularization‐Promoting Effect of Adenovirus Vector CI‐1023 in a Rat Hindlimb Ischaemic Model. Basic & Clinical Pharmacology & Toxicology 2004, 95: 76-80. PMID: 15379784, DOI: 10.1111/j.1742-7843.2004.950206.x.Peer-Reviewed Original ResearchConceptsHindlimb ischemic modelDose-dependent effectIschemic modelObvious dose-dependent effectDose rangeLocal tissue expressionVascular endothelial growth factor (VEGF) proteinReplication-deficient adenovirus vectorLaser Doppler scanningEfficacy of CIWide dose rangeGrowth factor proteinDoppler scanningImmuno-histochemistryTissue blood perfusionBlood perfusionTissue expressionAdenovirus vectorFactor proteinPrevious studiesAngiographyDelayed angiogenesis in aging rats and therapeutic effect of adenoviral gene transfer of VEGF.
Wang H, Keiser J, Olszewski B, Rosebury W, Robertson A, Kovesdi I, Gordon D. Delayed angiogenesis in aging rats and therapeutic effect of adenoviral gene transfer of VEGF. International Journal Of Molecular Medicine 2004, 13: 581-7. PMID: 15010860, DOI: 10.3892/ijmm.13.4.581.Peer-Reviewed Original ResearchAdenoviridaeAgingAnimalsBlotting, NorthernCell DivisionEndothelium, VascularEnzyme-Linked Immunosorbent AssayGene Transfer TechniquesHumansImmunoblottingImmunohistochemistryNeovascularization, PathologicNeovascularization, PhysiologicRatsTime FactorsTransfectionVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2Impaired angiogenesis in SHR is associated with decreased KDR and MT1-MMP expression
Wang H, Olszewski B, Rosebury W, Wang D, Robertson A, Keiser J. Impaired angiogenesis in SHR is associated with decreased KDR and MT1-MMP expression. Biochemical And Biophysical Research Communications 2004, 315: 363-368. PMID: 14766216, DOI: 10.1016/j.bbrc.2004.01.059.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsCell DivisionDNA, ComplementaryDown-RegulationEnzyme-Linked Immunosorbent AssayGene Transfer TechniquesHumansImmunoblottingImmunohistochemistryMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNeovascularization, PathologicProliferating Cell Nuclear AntigenRatsRats, Inbred SHRRats, Inbred WKYRats, Sprague-DawleySignal TransductionSpectrophotometryTime FactorsUp-RegulationVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ConceptsSpontaneous hypertensive ratsKinase insert domain-containing receptorMT1-MMP expressionAge-matched normotensive Wistar-KyotoImpaired angiogenesisEndothelial proliferationHypertension animal modelsNormotensive Wistar-KyotoSponge implantation modelLevel of angiogenesisSprague-Dawley ratsVEGF gene transferHypertensive ratsWistar KyotoDomain-containing receptorMembrane type 1 matrix metalloproteinaseAnimal modelsImmunohistological analysisMatrix metalloproteinaseImpaired VEGFSponge implantationAngiogenesisVEGF addition
2001
Expression of metalloproteinases and its inhibitor in later stage of rabbit neointima development.
Wang H, Liu C, Song Y, Gordon D, Alavi M, Moore S. Expression of metalloproteinases and its inhibitor in later stage of rabbit neointima development. International Journal Of Molecular Medicine 2001, 7: 105-12. PMID: 11115618, DOI: 10.3892/ijmm.7.1.105.Peer-Reviewed Original ResearchAnimalsBlotting, NorthernBlotting, WesternEndothelium, VascularGene Expression RegulationIn Situ HybridizationMaleMatrix Metalloproteinase 2Matrix Metalloproteinase 9MetalloendopeptidasesRabbitsRNA, MessengerTime FactorsTissue Inhibitor of Metalloproteinase-1Tissue Inhibitor of MetalloproteinasesTunica Intima
2000
Rat sponge implant model: a new system for evaluating angiogenic gene transfer.
Wang H, Gordon D, Olszewski B, Song Y, Kovesdi I, Keiser J. Rat sponge implant model: a new system for evaluating angiogenic gene transfer. International Journal Of Molecular Medicine 2000, 6: 645-53. PMID: 11078823, DOI: 10.3892/ijmm.6.6.645.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsBeta-GalactosidaseBlood VesselsDNA, RecombinantDose-Response Relationship, DrugEndothelial Growth FactorsGene Transfer TechniquesGene Transfer, HorizontalHumansImmunohistochemistryLymphokinesModels, CardiovascularNeovascularization, PathologicProstheses and ImplantsRatsRecombinant Fusion ProteinsSurgical SpongesTime FactorsVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsVon Willebrand FactorConceptsBeta-galactosidase reporter geneSuch genesReporter geneGenesGene transferTransfection efficiencyAngiogenic genesGene therapyProtein levelsProtein expressionVEGF121 geneReplication-deficient adenovirus vectorModel systemSponge implant modelAngiogenic responseProtein injectionAngiogenesisAdenovirus vectorVEGF protein expressionAngiogenic effectsProtein concentrationEnhanced angiogenesisAngiogenic efficacyGene transfection efficiencyHigh levels
1998
Molecular characterization of rabbit CPP32 and its function in vascular smooth muscle cell apoptosis
Wang H, Keiser J. Molecular characterization of rabbit CPP32 and its function in vascular smooth muscle cell apoptosis. American Journal Of Physiology 1998, 274: h1132-h1140. PMID: 9575916, DOI: 10.1152/ajpheart.1998.274.4.h1132.Peer-Reviewed Original ResearchConceptsCPP32 mRNASmooth muscle cellsDEVD-CHOSNP treatmentSignal transduction eventsCell death detection enzyme-linked immunosorbentDegradation of PARPApoptotic cell deathRabbit smooth muscle cellsVascular smooth muscle cell apoptosisCultured arterial smooth muscle cellsCaspase membersSmooth muscle cell apoptosisDNA fragmentation analysisMuscle cell apoptosisSubstrate recognitionTransduction eventsCPP32 proteinArterial smooth muscle cellsCultured smooth muscle cellsVascular smooth muscle cellsProtease familyDegenerate primersApoptosis pathwayCentral playerExpression of Membrane-Type Matrix Metalloproteinase in Rabbit Neointimal Tissue and Its Correlation with Matrix-Metalloproteinase-2 Activation
Wang H, Keiser J. Expression of Membrane-Type Matrix Metalloproteinase in Rabbit Neointimal Tissue and Its Correlation with Matrix-Metalloproteinase-2 Activation. Journal Of Vascular Research 1998, 35: 45-54. PMID: 9482695, DOI: 10.1159/000025564.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAortaBase SequenceBlotting, NorthernCells, CulturedDNA, ComplementaryEnzyme ActivationGelatinasesGene ExpressionHumansMaleMatrix Metalloproteinase 2Matrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMolecular Sequence DataMuscle, Smooth, VascularPolymerase Chain ReactionRabbitsRNA ProbesRNA, MessengerSequence HomologyConceptsBalloon catheter de-endothelializationMMP-2 activationArterial SMCsTemporal expression patternsMembrane-type matrix metalloproteinasesMembrane-type matrix metalloproteinaseNorthern blot analysisMatrix metalloproteinasesExtracellular matrix degradationRabbit cDNAMammalian cellsMT-MMP-1Matrix metalloproteinase-2 activationMT-MMP-1 expressionExpression patternsVascular neointimal formationSequence analysisMMP-2MMP cDNAPhenotypic alterationsCDNAPeak expressionRNA probesBlot analysisMatrix degradationCollagen biosynthesis by neointimal smooth muscle cells cultured from rabbit aortic explants 15 weeks after de‐endothelialization
WANG H, LI Z, MOORE S, ALAVI M. Collagen biosynthesis by neointimal smooth muscle cells cultured from rabbit aortic explants 15 weeks after de‐endothelialization. International Journal Of Experimental Pathology 1998, 79: 47-53. PMID: 9614349, PMCID: PMC3219429, DOI: 10.1046/j.1365-2613.1998.00048.x.Peer-Reviewed Original ResearchConceptsNeointimal smooth muscle cellsSmooth muscle cellsArterial neointimaCollagen accumulationMuscle cellsConfluent smooth muscle cellsBalloon catheter injuryExtracellular matrix accumulationCatheter injuryDe-endothelializationNormal aortaProcollagen IIIMatrix accumulationAortic neointimaMRNA expression
1997
Expression of syndecan-1 in rabbit neointima following de-endothelialization by a balloon catheter
Wang H, Moore S, Alavi M. Expression of syndecan-1 in rabbit neointima following de-endothelialization by a balloon catheter. Atherosclerosis 1997, 131: 141-147. PMID: 9199266, DOI: 10.1016/s0021-9150(97)06078-4.Peer-Reviewed Original ResearchConceptsSyndecan-1Low density lipoprotein retentionSyndecan-1 expressionArterial injuryBalloon catheterImmunohistochemical stainingEarly atherogenesisNeointimal cellsPositive cellsArterial neointimaRabbit aortaNormal aortaNeointimal tissueRabbit neointimaAortic neointimaNeointimaLipoprotein retentionCell membrane proteoglycansGrowth factorProtein levelsExtracellular matrix componentsNorthern blot analysisAortaBlot analysisInjury