2010
Improving the yield of circulating tumour cells facilitates molecular characterisation and recognition of discordant HER2 amplification in breast cancer
Flores LM, Kindelberger DW, Ligon AH, Capelletti M, Fiorentino M, Loda M, Cibas ES, Jänne PA, Krop IE. Improving the yield of circulating tumour cells facilitates molecular characterisation and recognition of discordant HER2 amplification in breast cancer. British Journal Of Cancer 2010, 102: 1495-1502. PMID: 20461092, PMCID: PMC2869174, DOI: 10.1038/sj.bjc.6605676.Peer-Reviewed Original ResearchConceptsHuman epidermal growth factor receptor 2Tumor cellsHER2 amplificationBreast cancerCTC yieldEpidermal growth factor receptor 2Growth factor receptor 2Breast cancer patientsSignificant discordance existsFactor receptor 2Lung cancer samplesMedian cell countMetastatic tumor cellsCancer patientsLung cancerMetastatic breastMetastatic tumorsPatient CTCsMetastatic cancerMetastatic tissuesBlood samplesReceptor 2Drug AdministrationCell countPatients
2009
Comparison of the Cellsearch Profile Kit (CPK) with the Standard Cellsearch Epithelial Kit (CEK) Demonstrates the CPK Produces Higher Circulating Tumor Cell (CTC) Yields and Is Better Suited for Use in Obtaining CTC's for Molecular Characterization.
Flores L, Kindelberger D, Ligon A, Cappeletti M, Fiorentino M, Loda M, Cibas E, Janne P, Krop I. Comparison of the Cellsearch Profile Kit (CPK) with the Standard Cellsearch Epithelial Kit (CEK) Demonstrates the CPK Produces Higher Circulating Tumor Cell (CTC) Yields and Is Better Suited for Use in Obtaining CTC's for Molecular Characterization. Cancer Research 2009, 69: 3003-3003. DOI: 10.1158/0008-5472.sabcs-09-3003.Peer-Reviewed Original ResearchML of bloodTumor cellsBreast cancerCytospin slidesLow intrapatient variabilityHER2 gene copy numberMetastatic breast cancerPeripheral blood samplesBC cell linesMedian cell countTumor cell yieldMean total numberMetastatic tumor cellsPrimary cancerCD45 stainingPredictive biomarkersMechanisms of resistanceHealthy controlsIntrapatient variabilityMetastatic cancerCTC yieldPharmacodynamic studiesSolid cancersCytokeratin stainingBlood samples