2022
Inhibition of NETosis by a Nuclear-Penetrating Anti-DNA Autoantibody
Chen X, Cuffari BJ, Dubljevic V, Shirali A, Zhou J, Campbell JA, Suits SC, O'Sullivan KM, Hansen JE. Inhibition of NETosis by a Nuclear-Penetrating Anti-DNA Autoantibody. ImmunoHorizons 2022, 6: 356-365. PMID: 35697479, DOI: 10.4049/immunohorizons.2100091.Peer-Reviewed Original ResearchConceptsNeutrophil extracellular trapsAnti-DNA autoantibodiesRelease of NETsInhibition of NETosisOncology clinical trialsDifferentiated PLB-985 cellsMechanisms of NETosisCalcium ionophore ionomycinPLB-985 cellsIndependent NETosisC57BL/6 miceClinical trialsExtracellular trapsSuch AbsAutoantibodiesNeutrophil biologyDNA damage responseTherapeutic potentialNETosisHistone citrullinationIonophore ionomycinWestern blotSignificant inhibitionMultiple diseasesNeutrophils
2015
DNA-dependent targeting of cell nuclei by a lupus autoantibody
Weisbart RH, Chan G, Jordaan G, Noble PW, Liu Y, Glazer PM, Nishimura RN, Hansen JE. DNA-dependent targeting of cell nuclei by a lupus autoantibody. Scientific Reports 2015, 5: 12022. PMID: 26156563, PMCID: PMC4496662, DOI: 10.1038/srep12022.Peer-Reviewed Original ResearchConceptsLupus anti-DNA autoantibodiesAnti-DNA autoantibodiesTumor cell nucleiSingle-chain variable fragmentPotential therapeutic applicationsLupus autoantibodiesTherapeutic approachesIschemic conditionsCertain cancer cellsChain variable fragmentCancer cellsCell nucleiAutoantibodiesNecrotic regionsVariable fragmentTherapeutic applicationsDNA damageNuclear uptakeScFvMalignancyGreater understandingTumorsStrokeDisease
2014
A nucleolytic lupus autoantibody is toxic to BRCA2-deficient cancer cells
Noble PW, Young MR, Bernatsky S, Weisbart RH, Hansen JE. A nucleolytic lupus autoantibody is toxic to BRCA2-deficient cancer cells. Scientific Reports 2014, 4: 5958. PMID: 25091037, PMCID: PMC5380011, DOI: 10.1038/srep05958.Peer-Reviewed Original ResearchConceptsLupus autoantibodiesTherapeutic agentsCancer cellsSystemic lupus erythematosusBRCA2-deficient cellsLupus erythematosusBRCA2-deficient cancer cellsDLD1 colon cancer cellsColon cancer cellsSuch antibodiesLower riskAutoantibodiesSpecific cancersAntibodiesMalignancyDifferential effectsToxic effectsPotential utilityCellsAgentsFurther supportDNA-damaging agentsErythematosusTherapyCancer
2007
Intranuclear Protein Transduction through a Nucleoside Salvage Pathway*
Hansen JE, Tse CM, Chan G, Heinze ER, Nishimura RN, Weisbart RH. Intranuclear Protein Transduction through a Nucleoside Salvage Pathway*. Journal Of Biological Chemistry 2007, 282: 20790-20793. PMID: 17525162, DOI: 10.1074/jbc.c700090200.Peer-Reviewed Original ResearchConceptsNucleoside salvage pathwaySalvage pathwaySpecific nuclear localizationCargo proteinsGene regulationMolecular delivery vehiclesProtein transportProtein transductionTranscription factorsNuclear localizationGene expressionNuclear membraneHuman diseasesLiving cellsCell nucleiCell membraneNew molecular therapiesNumerous diseasesPathwayTransductionCellsRegulationMolecular therapyFirst demonstrationNew avenues