2017
Acyl Histidines: New N‐Acyl Amides from Legionella pneumophila
Tørring T, Shames SR, Cho W, Roy CR, Crawford JM. Acyl Histidines: New N‐Acyl Amides from Legionella pneumophila. ChemBioChem 2017, 18: 638-646. PMID: 28116768, PMCID: PMC5546091, DOI: 10.1002/cbic.201600618.Peer-Reviewed Original ResearchConceptsBiosynthetic gene clusterOrphan biosynthetic gene clustersPathogen-insect interactionsAmino acid-derived metabolitesLegionella pneumophilaOrphan pathwaysBiosynthetic genesVariety of protozoanGene clusterN-acyl amidesMolecular networkingHuman macrophagesDistinct groupsPneumophilaCausative agentPathwayMetabolitesHistidine metabolitesNew N-acylGenesLegionnaires' diseaseAmoebaeProtozoaMacrophagesPathogens
2016
Linking Biosynthetic Gene Clusters to their Metabolites via Pathway-Targeted Molecular Networking
Trautman EP, Crawford JM. Linking Biosynthetic Gene Clusters to their Metabolites via Pathway-Targeted Molecular Networking. Current Topics In Medicinal Chemistry 2016, 16: 1705-1716. PMID: 26456470, PMCID: PMC5055756, DOI: 10.2174/1568026616666151012111046.Peer-Reviewed Original ResearchConceptsBiosynthetic gene clusterGene clusterMicrobial biosynthetic gene clustersMetabolic pathwaysGenome sequence informationSecondary metabolic pathwaysMolecular networkingHuman-microbe interactionsNew metabolic pathwaysComplex metabolomeSmall moleculesBiosynthetic logicActive small moleculesFunctional characterizationBioinformatics predictionSequence informationSmall molecule metabolitesHuman microbiomeExperimental structural characterizationGut bacteriaPathwayNatural productsMetabolitesPharmacological potentialAvailable databasesSecondary Metabolic Pathway-Targeted Metabolomics
Vizcaino MI, Crawford JM. Secondary Metabolic Pathway-Targeted Metabolomics. Methods In Molecular Biology 2016, 1401: 175-195. PMID: 26831709, PMCID: PMC5049693, DOI: 10.1007/978-1-4939-3375-4_12.Peer-Reviewed Original ResearchConceptsHigh-resolution mass spectrometryNovel natural product discoveryIsotopic labelingNatural product discovery effortsMicrobial natural product biosynthesisOrphan biosynthetic pathwaysComplex organic mixturesGlobal Natural Products Social Molecular Networking (GNPS) platformNatural product discoveryMS/MS acquisitionNatural product biosynthesisAnalytes of interestMS data acquisitionMS/MS data acquisitionNatural product extractionOrganic mixturesMolecular networkingNatural productsChemometric platformProduct discoveryMass spectrometryProduct biosynthesisMS acquisitionLiquid chromatographyMethod development