2021
A Conserved Nonribosomal Peptide Synthetase in Xenorhabdus bovienii Produces Citrulline-Functionalized Lipopeptides
Li JH, Cho W, Hamchand R, Oh J, Crawford JM. A Conserved Nonribosomal Peptide Synthetase in Xenorhabdus bovienii Produces Citrulline-Functionalized Lipopeptides. Journal Of Natural Products 2021, 84: 2692-2699. PMID: 34581573, PMCID: PMC9970011, DOI: 10.1021/acs.jnatprod.1c00573.Peer-Reviewed Original ResearchConceptsNonribosomal peptide synthetasePeptide synthetaseBiosynthetic gene clusterComparative genomic analysisFree-living infective juvenilesNRPS genesEffector proteinsInsect larvaeSpecialized metabolitesGene clusterMutualistic relationshipXenorhabdus bovieniiHeterologous expressionGenomic analysisRichest producersEntomopathogenic bacteriumSecondary metabolitesHost larvaeInfective juvenilesTermination domainInhibitor pathwayExogenous alcoholsLarvaeSpeciesComplex array
2020
Making and Breaking Leupeptin Protease Inhibitors in Pathogenic Gammaproteobacteria
Li J, Oh J, Kienesberger S, Kim N, Clarke D, Zechner E, Crawford J. Making and Breaking Leupeptin Protease Inhibitors in Pathogenic Gammaproteobacteria. Angewandte Chemie 2020, 132: 18028-18036. DOI: 10.1002/ange.202005506.Peer-Reviewed Original ResearchBacterial small moleculesTripeptide aldehyde protease inhibitorsHeterologous expression analysisProtease inhibitorsPhotorhabdus speciesColonization phenotypesHypothetical proteinsNematode hostsPathogenic GammaproteobacteriaExpression analysisBacterial productionAccessory enzymesGenetic distributionProteolytic degradationSmall moleculesEnzymeLeupeptinKlebsiella oxytocaInhibitorsLigasesXenorhabdusGammaproteobacteriaPhotorhabdusBiosynthesisSpeciesMaking and Breaking Leupeptin Protease Inhibitors in Pathogenic Gammaproteobacteria
Li J, Oh J, Kienesberger S, Kim NY, Clarke DJ, Zechner EL, Crawford J. Making and Breaking Leupeptin Protease Inhibitors in Pathogenic Gammaproteobacteria. Angewandte Chemie International Edition 2020, 59: 17872-17880. PMID: 32609431, DOI: 10.1002/anie.202005506.Peer-Reviewed Original ResearchConceptsBacterial small moleculesTripeptide aldehyde protease inhibitorsHeterologous expression analysisProtease inhibitorsColonization phenotypesPhotorhabdus speciesHypothetical proteinsNematode hostsPathogenic GammaproteobacteriaExpression analysisBacterial productionAccessory enzymesGenetic distributionProteolytic degradationSmall moleculesEnzymeLeupeptinKlebsiella oxytocaInhibitorsGammaproteobacteriaLigasesXenorhabdusPhotorhabdusBiosynthesisSpecies
2019
Bright Green Biofluorescence in Sharks Derives from Bromo-Kynurenine Metabolism
Park HB, Lam YC, Gaffney JP, Weaver JC, Krivoshik SR, Hamchand R, Pieribone V, Gruber DF, Crawford JM. Bright Green Biofluorescence in Sharks Derives from Bromo-Kynurenine Metabolism. IScience 2019, 19: 1291-1336. PMID: 31402257, PMCID: PMC6831821, DOI: 10.1016/j.isci.2019.07.019.Peer-Reviewed Original ResearchGreen fluorescent proteinGFP-like proteinsSpecies of sharksAcid-binding proteinFluorescence microscopy studiesFatty acid-binding proteinUndescribed groupSmall molecule metabolitesLuminescent phenotypeFluorescent proteinProteinMicrobial infectionsBiofluorescenceMarine environmentSharksSmall moleculesStructural detailsDiscovery of metabolitesWidespread natureCentral nervous systemNervous systemSpectral characterizationSpeciesMetabolitesDiversity
2018
Luciferin production and luciferase transcription in the bioluminescent copepod Metridia lucens
Tessler M, Gaffney JP, Crawford JM, Trautman E, Gujarati NA, Alatalo P, Pieribone VA, Gruber DF. Luciferin production and luciferase transcription in the bioluminescent copepod Metridia lucens. PeerJ 2018, 6: e5506. PMID: 30233994, PMCID: PMC6140675, DOI: 10.7717/peerj.5506.Peer-Reviewed Original ResearchOceanic food websParalog genesFood websUncharacterized speciesLuciferase transcriptionMolecular basisCommon speciesCopepod speciesMarine zooplanktonBright bioluminescenceLuciferase sequencesSpeciesCopepodsCopepod MetridiaCritical roleLight productionSouthern latitudesBioluminescenceMetridiaMass spectrometryL-phenylalanineTranscriptionL-tyrosineGenesZooplankton
2010
Siderophores from Neighboring Organisms Promote the Growth of Uncultured Bacteria
D'Onofrio A, Crawford JM, Stewart EJ, Witt K, Gavrish E, Epstein S, Clardy J, Lewis K. Siderophores from Neighboring Organisms Promote the Growth of Uncultured Bacteria. Cell Chemical Biology 2010, 17: 254-264. PMID: 20338517, PMCID: PMC2895992, DOI: 10.1016/j.chembiol.2010.02.010.Peer-Reviewed Original ResearchConceptsCulturing of organismsUncultured isolatesUncultured bacteriaGrowth factorGrowth promotionCultured microbesCommunity establishmentBacterial speciesSediment biofilmHelper strainSiderophoresCultured organismsOrganismsLack of growthBacteriaPetri dishesSynthetic mediumSame environmentHabitatsIsolatesMicrobesSpeciesGrowthStrainsPromiscuity