2019
Biocatalytic Reversal of Advanced Glycation End Product Modification
Kim NY, Goddard TN, Sohn S, Spiegel DA, Crawford J. Biocatalytic Reversal of Advanced Glycation End Product Modification. ChemBioChem 2019, 20: 2402-2410. PMID: 31013547, PMCID: PMC6768434, DOI: 10.1002/cbic.201900158.Peer-Reviewed Original ResearchConceptsImproved catalytic propertiesCondensation of sugarsLysine structureStructural homology analysisCatalytic propertiesSite-directed mutagenesisLead catalystFree amino acid formAcid formEnzyme variantsMaillard reactionHomology analysisCausal agentAuthentic ligandMolecular levelAdvanced glycation end product modificationAmino acid formMnmCLack of toolsAdvanced glycation end productsCatalystPeptidomimeticsLigandsVariantsMolecules
2014
An Atypical Orphan Carbohydrate-NRPS Genomic Island Encodes a Novel Lytic Transglycosylase
Guo X, Crawford JM. An Atypical Orphan Carbohydrate-NRPS Genomic Island Encodes a Novel Lytic Transglycosylase. Cell Chemical Biology 2014, 21: 1271-1277. PMID: 25219963, PMCID: PMC4224617, DOI: 10.1016/j.chembiol.2014.07.025.Peer-Reviewed Original ResearchConceptsGenome synteny analysisSynteny analysisGenomic islandsLytic transglycosylaseNatural product gene clustersOrphan biosynthetic pathwaysGene deletion analysisGenome sequencing platformsRare structural featureBiochemical reconstructionHypothetical proteinsGene clusterAcetyl-glucosamine moietyHeterologous expressionDeletion analysisProtein homologyBiosynthetic pathwayIsland contentNew small moleculesSequencing platformsMetabolic chemistryTransglycosylasePathwaySmall moleculesNatural products
2011
NRPS Substrate Promiscuity Diversifies the Xenematides
Crawford JM, Portmann C, Kontnik R, Walsh CT, Clardy J. NRPS Substrate Promiscuity Diversifies the Xenematides. Organic Letters 2011, 13: 5144-5147. PMID: 21888371, PMCID: PMC3184645, DOI: 10.1021/ol2020237.Peer-Reviewed Original Research
2008
Acyl‐Carrier Protein–Phosphopantetheinyltransferase Partnerships in Fungal Fatty Acid Synthases
Crawford JM, Vagstad AL, Ehrlich KC, Udwary DW, Townsend CA. Acyl‐Carrier Protein–Phosphopantetheinyltransferase Partnerships in Fungal Fatty Acid Synthases. ChemBioChem 2008, 9: 1559-1563. PMID: 18551496, PMCID: PMC3189688, DOI: 10.1002/cbic.200700659.Peer-Reviewed Original ResearchSynthetic Strategy of Nonreducing Iterative Polyketide Synthases and the Origin of the Classical “Starter‐Unit Effect”
Crawford JM, Vagstad AL, Whitworth KP, Ehrlich KC, Townsend CA. Synthetic Strategy of Nonreducing Iterative Polyketide Synthases and the Origin of the Classical “Starter‐Unit Effect”. ChemBioChem 2008, 9: 1019-1023. PMID: 18338425, PMCID: PMC3182100, DOI: 10.1002/cbic.200700702.Peer-Reviewed Original ResearchStarter unit specificity directs genome mining of polyketide synthase pathways in fungi
Crawford JM, Vagstad AL, Ehrlich KC, Townsend CA. Starter unit specificity directs genome mining of polyketide synthase pathways in fungi. Bioorganic Chemistry 2008, 36: 16-22. PMID: 18215412, PMCID: PMC3200548, DOI: 10.1016/j.bioorg.2007.11.002.Peer-Reviewed Original ResearchConceptsHybrid metabolitesPathogenic fungus Coccidioides immitisPolyketide synthase pathwayHuman fungal pathogenSAT domainAflatoxin clusterGenome miningSynthase subunitsPolyketide synthasePKS clustersACP domainStarter unitProtein databasePolyketide synthesisFungal pathogensFunctional similarityHexanoyl-CoA.Fatty acid productsEscherichia coliSynthase pathwayFungiFungus Coccidioides immitisAspergillus speciesPathwayDomain