An Ugi-like Biosynthetic Pathway Encodes Bombesin Receptor Subtype‑3 Agonists
Oh J, Kim NY, Chen H, Palm NW, Crawford JM. An Ugi-like Biosynthetic Pathway Encodes Bombesin Receptor Subtype‑3 Agonists. Journal Of The American Chemical Society 2019, 141: 16271-16278. PMID: 31537063, DOI: 10.1021/jacs.9b04183.Peer-Reviewed Original ResearchMeSH KeywordsCyanidesGlycopeptidesHEK293 CellsHumansModels, MolecularMolecular StructureReceptors, BombesinXenorhabdusCharacterization of a Hybrid Nonribosomal Peptide–Carbohydrate Biosynthetic Pathway in Photorhabdus luminescens
Perez CE, Crawford JM. Characterization of a Hybrid Nonribosomal Peptide–Carbohydrate Biosynthetic Pathway in Photorhabdus luminescens. Biochemistry 2019, 58: 1131-1140. PMID: 30694662, DOI: 10.1021/acs.biochem.8b01120.Peer-Reviewed Original ResearchConceptsHypothetical proteinsBiosynthetic pathwayNRPS machineryHybrid nonribosomal peptide synthetaseBacterial biosynthetic pathwaysStable isotope labelingNonribosomal peptide synthetaseProtein mass spectrometryEntomopathogen PhotorhabdusFascinating chemistryOrphan pathwaysCharacterization of oligosaccharidesAcetyl-glucosamine moietyProduction of metabolitesGenomic islandsGlycoamino acidsNovel chemistryGene clusterHeterologous expressionProtein biochemistryPeptide synthetaseBiological functionsGenome sequencingMass spectrometryIsotope labeling