2021
Chromosomal instability and aneuploidy as causes of cancer drug resistance
Lukow DA, Sheltzer JM. Chromosomal instability and aneuploidy as causes of cancer drug resistance. Trends In Cancer 2021, 8: 43-53. PMID: 34593353, DOI: 10.1016/j.trecan.2021.09.002.Peer-Reviewed Original ResearchConceptsChromosomal instabilityGene dosage alterationsChromosome copy number changesCopy number changesCell fitnessCancer drug resistanceCellular adaptabilitySelective pressureDrug resistanceTumor evolutionNumber changesDosage alterationsRecent evidenceAneuploidyIntratumoral heterogeneityPoor patient outcomesFitnessHigh levelsUnique vulnerabilitiesResistanceChromosomal instability accelerates the evolution of resistance to anti-cancer therapies
Lukow DA, Sausville EL, Suri P, Chunduri NK, Wieland A, Leu J, Smith JC, Girish V, Kumar AA, Kendall J, Wang Z, Storchova Z, Sheltzer JM. Chromosomal instability accelerates the evolution of resistance to anti-cancer therapies. Developmental Cell 2021, 56: 2427-2439.e4. PMID: 34352222, PMCID: PMC8933054, DOI: 10.1016/j.devcel.2021.07.009.Peer-Reviewed Original ResearchConceptsChromosomal instabilityAnti-cancer therapyCancer cell fitnessAcquisition of aneuploidyChromosome loss eventsSingle-cell sequencingEvolution of resistanceDifferent culture environmentsCellular fitnessPhenotypic plasticityCIN correlatesHuman tumorsCell fitnessHuman cellsStressful environmentsResistant populationsAcquisition of resistanceRecurrent aneuploidyCancer cellsPaclitaxel-resistant cellsCulture environmentAneuploidyPaclitaxel sensitivityFitnessCells
2020
A CRISPR Competition Assay to Identify Cancer Genetic Dependencies.
Girish V, Sheltzer JM. A CRISPR Competition Assay to Identify Cancer Genetic Dependencies. Bio-protocol 2020, 10: e3682. PMID: 33659353, PMCID: PMC7842800, DOI: 10.21769/bioprotoc.3682.Peer-Reviewed Original ResearchGenetic dependenciesTargeted locusCancer cell fitnessCRISPR/Cas9 systemGene of interestWhole-genome screenAnti-cancer drug developmentCell fitnessNuclease Cas9Mammalian cellsGenome editingGene dependenciesCas9 systemSpecific genesConsequences of lossCompetition assaysCell linesPotential targetGenesLociCancer typesDrug developmentFunction perturbationsCas9CRISPRDiscovering and validating cancer genetic dependencies: approaches and pitfalls
Lin A, Sheltzer JM. Discovering and validating cancer genetic dependencies: approaches and pitfalls. Nature Reviews Genetics 2020, 21: 671-682. PMID: 32561862, DOI: 10.1038/s41576-020-0247-7.Peer-Reviewed Original Research