2018
T cell LFA-1-induced proinflammatory mRNA stabilization is mediated by the p38 pathway kinase MK2 in a process regulated by hnRNPs C, H1 and K
Rao GK, Wong A, Collinge M, Sarhan J, Yarovinsky TO, Ramgolam VS, Gaestel M, Pardi R, Bender JR. T cell LFA-1-induced proinflammatory mRNA stabilization is mediated by the p38 pathway kinase MK2 in a process regulated by hnRNPs C, H1 and K. PLOS ONE 2018, 13: e0201103. PMID: 30048492, PMCID: PMC6065199, DOI: 10.1371/journal.pone.0201103.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Culture TechniquesCytoplasmELAV-Like Protein 1Heterogeneous-Nuclear RibonucleoproteinsHumansIntracellular Signaling Peptides and ProteinsJurkat CellsLymphocyte Function-Associated Antigen-1Mice, Inbred C57BLMice, KnockoutProtein Serine-Threonine KinasesProteomeRNA StabilityRNA, MessengerSignal TransductionT-LymphocytesConceptsKinase MK2Β2-integrin lymphocyte function-associated antigen-1AU-rich elementsLymphocyte function-associated antigen-1Integrin lymphocyte function-associated antigen-1HuR localizationProtein HuR.Key regulatorMRNA stabilizationCritical activatorCytoplasmic translocationHuR activitySequential activationHuRIntricate processFunction-associated antigen-1MRNAEngagement resultsMK2Antigen 1H1ActivationHnRNPsHuR.Transcripts
2010
T Cell LFA-1 Engagement Induces HuR-Dependent Cytokine mRNA Stabilization through a Vav-1, Rac1/2, p38MAPK and MKK3 Signaling Cascade
Ramgolam VS, DeGregorio SD, Rao GK, Collinge M, Subaran SS, Markovic-Plese S, Pardi R, Bender JR. T Cell LFA-1 Engagement Induces HuR-Dependent Cytokine mRNA Stabilization through a Vav-1, Rac1/2, p38MAPK and MKK3 Signaling Cascade. PLOS ONE 2010, 5: e14450. PMID: 21206905, PMCID: PMC3012057, DOI: 10.1371/journal.pone.0014450.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, SurfaceCytokinesELAV ProteinsELAV-Like Protein 1GTP PhosphohydrolasesHumansIntegrinsInterferon-gammaLymphocyte Function-Associated Antigen-1MAP Kinase Kinase 3MiceMice, Inbred C57BLNeuropeptidesP38 Mitogen-Activated Protein KinasesProto-Oncogene Proteins c-vavRac GTP-Binding ProteinsRac1 GTP-Binding ProteinRNA-Binding ProteinsSignal TransductionT-LymphocytesTumor Necrosis Factor-alpha
2006
LFA-1-Dependent HuR Nuclear Export and Cytokine mRNA Stabilization in T Cell Activation
Wang JG, Collinge M, Ramgolam V, Ayalon O, Fan XC, Pardi R, Bender JR. LFA-1-Dependent HuR Nuclear Export and Cytokine mRNA Stabilization in T Cell Activation. The Journal Of Immunology 2006, 176: 2105-2113. PMID: 16455966, DOI: 10.4049/jimmunol.176.4.2105.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAntigens, SurfaceBase SequenceCD28 AntigensCell NucleusCells, CulturedCytokinesCytoplasmELAV ProteinsELAV-Like Protein 1HumansLymphocyte ActivationLymphocyte Function-Associated Antigen-1Molecular Sequence DataRNA StabilityRNA, MessengerRNA, Small InterferingRNA-Binding ProteinsT-LymphocytesConceptsNuclear exportAU-rich element (ARE) sequenceMRNA stabilizationClass II AU-rich elementsT cell activationAU-rich elementsLymphokine gene expressionRapid nuclearRegulated processIntegrin engagementCell activationMRNA reporterRNA interferenceHuR functionIntegrin LFA-1MRNA stabilityGene expressionProtein productionHuman peripheral T cellsCytoplasmic translocationGM-CSF mRNAElement sequencesProtein HuRHuR levelsLFA-1
2005
Dynamic Partitioning into Lipid Rafts Controls the Endo-Exocytic Cycle of the αL/β2 Integrin, LFA-1, during Leukocyte Chemotaxis
Fabbri M, Di Meglio S, Gagliani MC, Consonni E, Molteni R, Bender JR, Tacchetti C, Pardi R. Dynamic Partitioning into Lipid Rafts Controls the Endo-Exocytic Cycle of the αL/β2 Integrin, LFA-1, during Leukocyte Chemotaxis. Molecular Biology Of The Cell 2005, 16: 5793-5803. PMID: 16207819, PMCID: PMC1289422, DOI: 10.1091/mbc.e05-05-0413.Peer-Reviewed Original ResearchMeSH KeywordsCells, CulturedChemotaxis, LeukocyteEndocytosisExocytosisHumansLymphocyte Function-Associated Antigen-1Membrane MicrodomainsNeutrophilsProtein TransportReference ValuesConceptsDetergent-resistant membranesInternalized receptorsLate endosomal pathwayCholesterol-sequestering agentRab11 mutantDRM associationPolarized recyclingCell rearCell frontPlasma membraneChemoattractant stimulationEndocytic compartmentsEndosomal pathwayAdhesion receptorsCell migrationNew protrusionsIntegrinsLFA-1Dynamic redistributionMutantsLamellipodiaAdhesive interactionsPrimary neutrophilsIntracellular accumulationΒ2 integrins
2000
CD28 and LFA‐1 contribute to cyclosporin A‐resistant T cell growth by stabilizing the IL‐2 mRNA through distinct signaling pathways
Geginat J, Clissi B, Moro M, Dellabona P, Bender J, Pardi R. CD28 and LFA‐1 contribute to cyclosporin A‐resistant T cell growth by stabilizing the IL‐2 mRNA through distinct signaling pathways. European Journal Of Immunology 2000, 30: 1136-1144. PMID: 10760803, DOI: 10.1002/(sici)1521-4141(200004)30:4<1136::aid-immu1136>3.0.co;2-3.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDB7-2 AntigenCalcineurinCD28 AntigensCells, CulturedCyclosporineCytoskeletonDendritic CellsDNA-Binding ProteinsDrug SynergismHumansIntercellular Adhesion Molecule-1Interleukin-2Lymphocyte ActivationLymphocyte Function-Associated Antigen-1Membrane GlycoproteinsMitogen-Activated Protein KinasesNF-kappa BNFATC Transcription FactorsNuclear ProteinsPromoter Regions, GeneticProtein BindingRNA StabilityRNA, MessengerSignal TransductionSuperantigensT-LymphocytesTranscription FactorsConceptsIL-2 mRNALFA-1ICAM-1IL-2 dependentT cell proliferationSubsequent T cell proliferationCostimulatory molecule CD28TCR-induced proliferationSignaling pathwaysT cell growthIL-2 transcriptsGraft rejectionDendritic cellsIL-2Clinical transplantationT lymphocytesMolecule CD28Primary T lymphocytesNF-kappaBCD28Distinct signaling pathwaysLower transcriptional rateDifferent signaling pathwaysProtein kinase activationCell proliferation
1999
Anchorage dependence of mitogen-induced G1 to S transition in primary T lymphocytes.
Geginat J, Bossi G, Bender J, Pardi R. Anchorage dependence of mitogen-induced G1 to S transition in primary T lymphocytes. The Journal Of Immunology 1999, 162: 5085-93. PMID: 10227977, DOI: 10.4049/jimmunol.162.9.5085.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalCalcium-Calmodulin-Dependent Protein KinasesCell AdhesionCell CycleCell Cycle ProteinsCell SizeCyclin-Dependent Kinase 4Cyclin-Dependent Kinase 6Cyclin-Dependent Kinase Inhibitor p27Cyclin-Dependent KinasesDown-RegulationEnzyme ActivationG1 PhaseGene Expression RegulationGenes, fosGenes, junHumansInterleukin-2InterphaseKineticsLymphocyte Function-Associated Antigen-1Microtubule-Associated ProteinsMitogensProtein Serine-Threonine KinasesProto-Oncogene ProteinsReceptors, Antigen, T-CellS PhaseT-LymphocytesTumor Suppressor ProteinsConceptsNormal T cellsT lymphocytesT cellsPrimary T lymphocytesRetinoblastoma protein inactivationCytokines IL-2Function-blocking mAbsIL-2ICAM-1Mitogen-activated protein kinase activationCyclin-dependent kinase inhibitor p27kipIntegrins actMitogenic responseMitogenic cytokinesGrowth factorLymphocytesCell cycle progressionTCR stimulationLate componentsProtein kinase activationLeukocyte integrinsAnchorage dependenceTCR triggeringCycle progressionCellular requirements
1996
Feedback modulation of ligand-engaged alpha L/beta 2 leukocyte integrin (LFA-1) by cyclic AMP-dependent protein kinase.
Rovere P, Inverardi L, Bender J, Pardi R. Feedback modulation of ligand-engaged alpha L/beta 2 leukocyte integrin (LFA-1) by cyclic AMP-dependent protein kinase. The Journal Of Immunology 1996, 156: 2273-9. PMID: 8690918, DOI: 10.4049/jimmunol.156.6.2273.Peer-Reviewed Original ResearchConceptsProtein kinase CAdhesion receptorsKinase CCyclic AMP-dependent protein kinaseAMP-dependent protein kinaseCytoskeletal anchoring proteinsIntegrin-dependent activationCAMP-dependent kinase activationIntracellular cAMP elevationCAMP elevationHeterologous cell linesLeukocyte integrinsAnchoring proteinsRegulated processProtein kinaseAdenylyl cyclase isoformsMolecular basisKinase activationIntercellular adhesionF-actinCell deadhesionHuman intercellular adhesion molecule-1LFA-1 receptorsDependent adhesionShort-term regulation
1995
Conserved regions in the cytoplasmic domains of the leukocyte integrin alpha L beta 2 are involved in endoplasmic reticulum retention, dimerization, and cytoskeletal association.
Pardi R, Bossi G, Inverardi L, Rovida E, Bender J. Conserved regions in the cytoplasmic domains of the leukocyte integrin alpha L beta 2 are involved in endoplasmic reticulum retention, dimerization, and cytoskeletal association. The Journal Of Immunology 1995, 155: 1252-63. PMID: 7636193, DOI: 10.4049/jimmunol.155.3.1252.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric RegulationAmino Acid SequenceAnimalsCell CompartmentationCell Line, TransformedChlorocebus aethiopsCytoskeletonEndoplasmic ReticulumLymphocyte Function-Associated Antigen-1Molecular Sequence DataProtein ConformationProtein MultimerizationProtein Structure, TertiaryRecombinant Fusion ProteinsSequence DeletionT-LymphocytesTetradecanoylphorbol AcetateTransfectionConceptsAlpha L beta 2Cytoplasmic domainEndoplasmic reticulum retentionCytoskeletal associationBeta subunitAlpha L subunitAlpha beta complexFunction of integrinsBeta 2GFFKR motifAdherent cellsDeletion mutantsSubstitution mutantsEctopic expressionIntegrin alphaPlasma membraneHeterodimer formationConserved regionsAdhesion receptorsBeta complexCytoplasmic truncationRegulated functionsL subunitsStructural determinantsAlpha L
1992
Early recognition of a discordant xenogeneic organ by human circulating lymphocytes.
Inverardi L, Samaja M, Motterlini R, Mangili F, Bender J, Pardi R. Early recognition of a discordant xenogeneic organ by human circulating lymphocytes. The Journal Of Immunology 1992, 149: 1416-23. PMID: 1386865, DOI: 10.4049/jimmunol.149.4.1416.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, DifferentiationAntigens, Differentiation, T-LymphocyteCD3 ComplexCell AdhesionCytotoxicity, ImmunologicEndothelium, VascularHeart TransplantationHumansImmunity, CellularKiller Cells, NaturalLymphocyte Function-Associated Antigen-1Lymphocyte SubsetsMyocardiumRatsReceptors, Antigen, T-CellReceptors, FcReceptors, IgGTransplantation, HeterologousConceptsNK cellsXenogeneic organsCell-mediated immune mechanismsHuman lymphocytesAntibody-independent pathwayLymphocyte phenotypic analysisCell-mediated mechanismsHuman NK cellsNK cell adhesionPretreatment of lymphocytesAnti-CD18 antibodiesDiscordant xenograft rejectionHuman circulating lymphocytesEx vivo modelRat endothelial cellsCD3-CD16Vascular resistanceLymphocyte subsetsXenograft rejectionCirculating lymphocytesCoronary systemEarly recognitionImmune mechanismsT lymphocytesCell sequestrationAntigen-receptor complex stimulation triggers protein kinase C-dependent CD11a/CD18-cytoskeleton association in T lymphocytes.
Pardi R, Inverardi L, Rugarli C, Bender J. Antigen-receptor complex stimulation triggers protein kinase C-dependent CD11a/CD18-cytoskeleton association in T lymphocytes. Journal Of Cell Biology 1992, 116: 1211-1220. PMID: 1346786, PMCID: PMC2289356, DOI: 10.1083/jcb.116.5.1211.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdultAntigens, CDAntigens, Differentiation, T-LymphocyteCD18 AntigensCD3 ComplexChild, PreschoolCytoskeletal ProteinsCytoskeletonHumansLymphocyte ActivationLymphocyte Function-Associated Antigen-1PhosphorylationProtein Kinase CReceptors, Antigen, T-CellSignal TransductionT-LymphocytesConceptsProtein kinase C inhibitor staurosporineProtein kinase CT cell receptor complexCell receptor complexC inhibitor staurosporineHigh avidity stateSignal transductionCytoskeletal rearrangementsActin polymerizationIntercellular adhesionIntracellular signalsF-actinMolecular eventsInhibitor staurosporineKinase CAvidity stateLFA-1Membrane fractionCD11a/CD18 complexTCR complexTCR crosslinkingPKC desensitizationComplex activationReceptor complexFluorescence microscopy
1989
Heterogeneous distribution and transmembrane signaling properties of lymphocyte function-associated antigen (LFA-1) in human lymphocyte subsets.
Pardi R, Bender J, Dettori C, Giannazza E, Engleman E. Heterogeneous distribution and transmembrane signaling properties of lymphocyte function-associated antigen (LFA-1) in human lymphocyte subsets. The Journal Of Immunology 1989, 143: 3157-66. PMID: 2553807, DOI: 10.4049/jimmunol.143.10.3157.Peer-Reviewed Original ResearchMeSH KeywordsAdultAntigens, DifferentiationCell AdhesionCell MembraneCross-Linking ReagentsEndothelium, VascularHumansHydrolysisInterphaseLymphocyte Function-Associated Antigen-1LymphocytesMembrane GlycoproteinsN-Acetylneuraminic AcidPhosphatidylinositolsReceptors, Leukocyte-AdhesionSialic AcidsSignal TransductionStructure-Activity RelationshipConceptsLymphocyte subsetsLFA-1Lymphocyte function-associated antigenDifferent lymphocyte subsetsHuman lymphocyte subsetsFunction-associated antigenAnti-beta subunit antibodiesCell surface densityPhosphoinositide hydrolysisSialic acid contentSubunit antibodiesVariable expressionIntegrin supergene familyLFA-1 alpha subunitNatural ligandCell bindingNeuraminidase digestionActivation eventsRelative increaseAlpha subunitSupergene familySubsetBeta subunit
1987
Lymphocyte subsets differentially induce class II human leukocyte antigens on allogeneic microvascular endothelial cells.
Pardi R, Bender J, Engleman E. Lymphocyte subsets differentially induce class II human leukocyte antigens on allogeneic microvascular endothelial cells. The Journal Of Immunology 1987, 139: 2585-92. PMID: 3116086, DOI: 10.4049/jimmunol.139.8.2585.Peer-Reviewed Original ResearchConceptsClass II human leukocyteMicrovascular endothelial cellsEndothelial cellsT cellsAllogeneic T cell proliferationMajor histocompatibility complex class IIHuman leukocytesHistocompatibility complex class IILymphocyte-EC adhesionT-cell surface antigensNatural killer cellsT cell proliferationHost immune responseHuman microvascular endothelial cellsCell surface antigensAcute rejectionAntilymphocyte antibodiesGraft alloantigensLymphocyte subsetsAllogeneic lymphocytesAllograft rejectionLymphocyte subpopulationsKiller cellsAutologous monocytesCD18 antibody