2000
Human vascular endothelial cells contain membrane binding sites for estradiol, which mediate rapid intracellular signaling
Russell K, Haynes M, Sinha D, Clerisme E, Bender J. Human vascular endothelial cells contain membrane binding sites for estradiol, which mediate rapid intracellular signaling. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 5930-5935. PMID: 10823945, PMCID: PMC18536, DOI: 10.1073/pnas.97.11.5930.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalCell LineCell Membrane PermeabilityCells, CulturedCyclic GMPEndothelium, VascularEstradiolFlow CytometryHumansMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesNitric OxideReceptors, EstrogenRecombinant Fusion ProteinsSerum Albumin, BovineSignal TransductionTime FactorsTransfectionConceptsHuman endothelial cellsEstrogen receptorEndothelial cellsMitogen-activated protein kinaseER antagonist ICIHuman vascular endothelial cellsMembrane estrogen receptorsMembrane-impermeant formVascular endothelial cellsRapid intracellularRapid nongenomic responsesAntagonist ICIVascular functionE2 treatmentAcute effectsERalpha antibodyCGMP productionCardiovascular systemNongenomic responsesCytometric analysisEstrogen inducesGuanylate cyclaseRapid effectsNO releaseEarly effects
1999
Anchorage dependence of mitogen-induced G1 to S transition in primary T lymphocytes.
Geginat J, Bossi G, Bender J, Pardi R. Anchorage dependence of mitogen-induced G1 to S transition in primary T lymphocytes. The Journal Of Immunology 1999, 162: 5085-93. PMID: 10227977, DOI: 10.4049/jimmunol.162.9.5085.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalCalcium-Calmodulin-Dependent Protein KinasesCell AdhesionCell CycleCell Cycle ProteinsCell SizeCyclin-Dependent Kinase 4Cyclin-Dependent Kinase 6Cyclin-Dependent Kinase Inhibitor p27Cyclin-Dependent KinasesDown-RegulationEnzyme ActivationG1 PhaseGene Expression RegulationGenes, fosGenes, junHumansInterleukin-2InterphaseKineticsLymphocyte Function-Associated Antigen-1Microtubule-Associated ProteinsMitogensProtein Serine-Threonine KinasesProto-Oncogene ProteinsReceptors, Antigen, T-CellS PhaseT-LymphocytesTumor Suppressor ProteinsConceptsNormal T cellsT lymphocytesT cellsPrimary T lymphocytesRetinoblastoma protein inactivationCytokines IL-2Function-blocking mAbsIL-2ICAM-1Mitogen-activated protein kinase activationCyclin-dependent kinase inhibitor p27kipIntegrins actMitogenic responseMitogenic cytokinesGrowth factorLymphocytesCell cycle progressionTCR stimulationLate componentsProtein kinase activationLeukocyte integrinsAnchorage dependenceTCR triggeringCycle progressionCellular requirements
1990
T-cell receptor-negative natural killer cells display antigen-specific cytotoxicity for microvascular endothelial cells.
Bender J, Pardi R, Engleman E. T-cell receptor-negative natural killer cells display antigen-specific cytotoxicity for microvascular endothelial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 6949-6953. PMID: 2402485, PMCID: PMC54659, DOI: 10.1073/pnas.87.18.6949.Peer-Reviewed Original ResearchConceptsMicrovascular endothelial cellsEndothelial cellsNK cellsNatural killer cell targetsNatural killer cellsAllogeneic endothelial cellsAntigen-specific cytotoxicityNK cell linesCD3/T cell receptorLevel of adherenceEC linesHuman microvascular endothelial cellsT cell receptorOrgan allograftsGamma chain geneKiller cellsLymphoid cellsSpecific cytotoxicityCell targetsNegative cellsCell linesHuman erythroleukemia cellsSelective cytotoxicityTi moleculesCytotoxicityNatural killer lines and clones with apparent antigen specificity.
Suzuki N, Bianchi E, Bass H, Suzuki T, Bender J, Pardi R, Brenner C, Larrick J, Engleman E. Natural killer lines and clones with apparent antigen specificity. Journal Of Experimental Medicine 1990, 172: 457-462. PMID: 2142719, PMCID: PMC2188332, DOI: 10.1084/jem.172.2.457.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntigen-Antibody ComplexAntigens, CDAntigens, DifferentiationAntigens, Differentiation, T-LymphocyteCD3 ComplexCell LineCells, CulturedClone CellsCytotoxicity, ImmunologicFlow CytometryHumansKiller Cells, NaturalMacromolecular SubstancesReceptors, Antigen, T-CellReceptors, FcReceptors, IgGConceptsLymphoblastoid cell linesAllogeneic lymphoblastoid cell linesTCR alpha/betaClass II MHC antigensIL-2-containing mediumPotent cytolytic activityNK cellsAllogeneic targetsCD3 lymphocytesMHC antigensCytolytic activityAntigen specificityTCR-beta transcriptsCD3Class ICD16CD3 epsilonSelective killingNorthern blot analysisBlot analysisCell linesK562 cellsK562 erythroleukemia cellsChain transcriptsMAbs
1987
Lymphocyte subsets differentially induce class II human leukocyte antigens on allogeneic microvascular endothelial cells.
Pardi R, Bender J, Engleman E. Lymphocyte subsets differentially induce class II human leukocyte antigens on allogeneic microvascular endothelial cells. The Journal Of Immunology 1987, 139: 2585-92. PMID: 3116086, DOI: 10.4049/jimmunol.139.8.2585.Peer-Reviewed Original ResearchConceptsClass II human leukocyteMicrovascular endothelial cellsEndothelial cellsT cellsAllogeneic T cell proliferationMajor histocompatibility complex class IIHuman leukocytesHistocompatibility complex class IILymphocyte-EC adhesionT-cell surface antigensNatural killer cellsT cell proliferationHost immune responseHuman microvascular endothelial cellsCell surface antigensAcute rejectionAntilymphocyte antibodiesGraft alloantigensLymphocyte subsetsAllogeneic lymphocytesAllograft rejectionLymphocyte subpopulationsKiller cellsAutologous monocytesCD18 antibodyPhenotypic and functional characterization of lymphocytes that bind human microvascular endothelial cells in vitro. Evidence for preferential binding of natural killer cells.
Bender J, Pardi R, Karasek M, Engleman E. Phenotypic and functional characterization of lymphocytes that bind human microvascular endothelial cells in vitro. Evidence for preferential binding of natural killer cells. Journal Of Clinical Investigation 1987, 79: 1679-1688. PMID: 3495552, PMCID: PMC424501, DOI: 10.1172/jci113007.Peer-Reviewed Original ResearchConceptsNatural killer cellsMicrovascular endothelial cellsEndothelial cellsKiller cellsE-RFCRosette forming lymphocytesRecombinant interleukin-1Human microvascular endothelial cellsReceptor-ligand interactionsLymphocyte subsetsNK effectorsNK targetsGraft rejectionNK cellsLeu-11Lymphocyte fractionInterleukin-1Microvascular endotheliumSpecific receptor-ligand interactionsAllograftsLymphocytesEC monolayersCritical targetCellsQuantitative adherence