2022
Zebrafish models of Alx-linked frontonasal dysplasia reveal a role for Alx1 and Alx3 in the anterior segment and vasculature of the developing eye
Yoon B, Yeung P, Santistevan N, Bluhm L, Kawasaki K, Kueper J, Dubielzig R, Vanoudenhove J, Cotney J, Liao E, Grinblat Y. Zebrafish models of Alx-linked frontonasal dysplasia reveal a role for Alx1 and Alx3 in the anterior segment and vasculature of the developing eye. Biology Open 2022, 11: bio059189. PMID: 35142342, PMCID: PMC9167625, DOI: 10.1242/bio.059189.Peer-Reviewed Original ResearchConceptsALX geneAnterior neurocraniumZebrafish modelGenetic mechanismsNovel roleAnterior segment formationHomeobox transcription factorCranial neural crestOxidative stress responseParalogous genesConserved roleAnterior segment defectsAbsence of eyesEthanol toxicityTranscription factorsTranscriptomic analysisLineage labelingAlx1Midfacial morphogenesisKey regulatorNeural crestStress responseSegment formationMutantsVascular development
2017
Precocious Phenotypic Transcription‐Factor Expression During Early Development
VanOudenhove J, Medina R, Ghule P, Lian J, Stein J, Zaidi S, Stein G. Precocious Phenotypic Transcription‐Factor Expression During Early Development. Journal Of Cellular Biochemistry 2017, 118: 953-958. PMID: 27591551, PMCID: PMC5336526, DOI: 10.1002/jcb.25723.Peer-Reviewed Original ResearchConceptsPhenotypic transcription factorsTranscription factorsPrecocious expressionRUNX1 transcription factorTranscriptional controlLineage identityGene expressionNovel roleBiological importanceMesenchymal transitionEarly differentiationMesenchymal differentiationTransient upregulationDetailed mechanistic studiesExpressionDifferentiationMechanistic studiesRUNX1RoleUpregulationFurther studies
2016
Transient RUNX1 Expression during Early Mesendodermal Differentiation of hESCs Promotes Epithelial to Mesenchymal Transition through TGFB2 Signaling
VanOudenhove J, Medina R, Ghule P, Lian J, Stein J, Zaidi S, Stein G. Transient RUNX1 Expression during Early Mesendodermal Differentiation of hESCs Promotes Epithelial to Mesenchymal Transition through TGFB2 Signaling. Stem Cell Reports 2016, 7: 884-896. PMID: 27720906, PMCID: PMC5106514, DOI: 10.1016/j.stemcr.2016.09.006.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsHESC differentiationMesendodermal lineage commitmentPhenotypic transcription factorsLoss of repressionRUNX1 depletionMesenchymal transitionEmbryonic stem cellsLoss of RUNX1Mesendodermal lineagesMesendodermal differentiationLineage commitmentTranscription factorsBiochemical approachesEpithelial genesCell motilityFunctional analysisEpithelial marker expressionRUNX1 expressionStem cellsRUNX1TGFB2DifferentiationEarly eventsCandidate factorsMicroRNA-378-mediated suppression of Runx1 alleviates the aggressive phenotype of triple-negative MDA-MB-231 human breast cancer cells
Browne G, Dragon J, Hong D, Messier T, Gordon J, Farina N, Boyd J, VanOudenhove J, Perez A, Zaidi S, Stein J, Stein G, Lian J. MicroRNA-378-mediated suppression of Runx1 alleviates the aggressive phenotype of triple-negative MDA-MB-231 human breast cancer cells. Tumor Biology 2016, 37: 8825-8839. PMID: 26749280, PMCID: PMC4939137, DOI: 10.1007/s13277-015-4710-6.Peer-Reviewed Original ResearchConceptsMDA-MB-231 cellsBreast cancer progressionMiR-378Breast cancer cellsCancer progressionMMTV-PyMT transgenic mouse modelCancer cellsLuciferase reporter assaysMiRNA replacement therapyMDA-MB-231 human breast cancer cellsBreast cancerTranscription factorsNumerous miRNAsEctopic expressionRegulatory pathwaysUntranslated regionMicroarray profilingReporter assaysHuman breast cancer cellsTriple-negative MDA-MB-231Breast cancer cell line MCF7Human breast cancer cell line MCF7Cell migrationRUNX1 expressionNormal hematopoiesis