2017
Unique Regulatory Mechanisms for the Human Embryonic Stem Cell Cycle
VanOudenhove J, Grandy R, Ghule P, Lian J, Stein J, Zaidi S, Stein G. Unique Regulatory Mechanisms for the Human Embryonic Stem Cell Cycle. Journal Of Cellular Physiology 2017, 232: 1254-1257. PMID: 27532275, PMCID: PMC5315681, DOI: 10.1002/jcp.25567.Peer-Reviewed Original ResearchMeSH KeywordsCell CycleCell DifferentiationCell LineageHuman Embryonic Stem CellsHumansModels, BiologicalConceptsCell cyclePluripotent human embryonic stem cellsPluripotent cell cyclePluripotent cell populationHuman embryonic stem cellsEmbryonic stem cellsG1 periodCell cycle pauseMesodermal lineage cellsEpigenetic mechanismsEctodermal differentiationRegulatory eventsUnrestricted proliferationLineage cellsStem cellsCell populationsUnique mechanismRecent findingsDifferentiationPluripotencyCellsMechanismProliferationCycle
2016
Transient RUNX1 Expression during Early Mesendodermal Differentiation of hESCs Promotes Epithelial to Mesenchymal Transition through TGFB2 Signaling
VanOudenhove J, Medina R, Ghule P, Lian J, Stein J, Zaidi S, Stein G. Transient RUNX1 Expression during Early Mesendodermal Differentiation of hESCs Promotes Epithelial to Mesenchymal Transition through TGFB2 Signaling. Stem Cell Reports 2016, 7: 884-896. PMID: 27720906, PMCID: PMC5106514, DOI: 10.1016/j.stemcr.2016.09.006.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsHESC differentiationMesendodermal lineage commitmentPhenotypic transcription factorsLoss of repressionRUNX1 depletionMesenchymal transitionEmbryonic stem cellsLoss of RUNX1Mesendodermal lineagesMesendodermal differentiationLineage commitmentTranscription factorsBiochemical approachesEpithelial genesCell motilityFunctional analysisEpithelial marker expressionRUNX1 expressionStem cellsRUNX1TGFB2DifferentiationEarly eventsCandidate factorsGenome-Wide Studies Reveal that H3K4me3 Modification in Bivalent Genes Is Dynamically Regulated during the Pluripotent Cell Cycle and Stabilized upon Differentiation
Grandy R, Whitfield T, Wu H, Fitzgerald M, VanOudenhove J, Zaidi S, Montecino M, Lian J, van Wijnen A, Stein J, Stein G. Genome-Wide Studies Reveal that H3K4me3 Modification in Bivalent Genes Is Dynamically Regulated during the Pluripotent Cell Cycle and Stabilized upon Differentiation. Molecular And Cellular Biology 2016, 36: 615-627. PMID: 26644406, PMCID: PMC4751694, DOI: 10.1128/mcb.00877-15.Peer-Reviewed Original ResearchMeSH KeywordsCell CycleCell DifferentiationCell LineChromatinDNA MethylationDNA-Binding ProteinsEpigenesis, GeneticGene Expression Regulation, DevelopmentalGenome-Wide Association StudyHistone-Lysine N-MethyltransferaseHistonesHuman Embryonic Stem CellsHumansMyeloid-Lymphoid Leukemia ProteinNeoplasm ProteinsConceptsHuman embryonic stem cellsBivalent genesHistone modificationsCell cycleCell cycle-dependent fashionPluripotent cell cycleRepressive histone modificationsPosttranslational histone modificationsH3K4me3/H3K27me3Maintenance of pluripotencyHistone modification signaturesMethylation/demethylationLevels of H3K4me3Embryonic stem cellsInduction of differentiationChromatin regulationChromatin modifiersEpigenetic landscapeCell identityModification signaturesLineage commitmentGenomic enrichmentGene promoterProgeny cellsMolecular mechanisms