2022
In situ architecture of the lipid transport protein VPS13C at ER–lysosome membrane contacts
Cai S, Wu Y, Guillén-Samander A, Hancock-Cerutti W, Liu J, De Camilli P. In situ architecture of the lipid transport protein VPS13C at ER–lysosome membrane contacts. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2203769119. PMID: 35858323, PMCID: PMC9303930, DOI: 10.1073/pnas.2203769119.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumCryo-focused ion beam millingMembrane contact sitesCryo-electron tomographyFull-length structureLipid transport proteinsRod-like densitiesLysosome contactBinding partnerGenetic approachesHydrophobic grooveTransport proteinsContact sitesVps13Lipid transportAlphaFold predictionsFull-length modelHeLa cellsMembrane contactSitu architectureAdjacent membranesVPS13CProteinStructural informationEndo/lysosomes
2017
Molecular architecture of the sheathed polar flagellum in Vibrio alginolyticus
Zhu S, Nishikino T, Hu B, Kojima S, Homma M, Liu J. Molecular architecture of the sheathed polar flagellum in Vibrio alginolyticus. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: 10966-10971. PMID: 28973904, PMCID: PMC5642721, DOI: 10.1073/pnas.1712489114.Peer-Reviewed Original ResearchConceptsPolar flagellumOuter membraneIntact cellsFlagellar motor componentsSheathed polar flagellumRod-shaped bacteriaGram-negative rod-shaped bacteriaLimited structural informationPeriplasmic structureSubtomogram analysisFlagellar rotationFlagellar motorGenetic approachesUnsheathed flagellaCryoelectron tomographySwimming motilitySheathed flagellumMajor remodelingFlagellaRing-like structureMolecular levelDistinct motilityFast motilityMembranous sheathLength variation