2022
Exploitation of a Bacterium-Encoded Lytic Transglycosylase by a Human Oral Lytic Phage To Facilitate Infection
Cen L, Chang Y, Bedree JK, Ma Y, Zhong Q, Utter DR, Dong PT, Lux R, Bor B, Liu J, McLean JS, Le S, He X. Exploitation of a Bacterium-Encoded Lytic Transglycosylase by a Human Oral Lytic Phage To Facilitate Infection. Journal Of Virology 2022, 96: e01063-22. PMID: 36000841, PMCID: PMC9472602, DOI: 10.1128/jvi.01063-22.Peer-Reviewed Original ResearchConceptsLytic transglycosylaseLytic phagesPhage receptorsOral phageRemarkable host specificityHuman oral microbiomeSimilar expression levelsPeptidoglycan remodelingWhole-genome sequencingMutant backgroundBacterial physiologyHost specificityMicrobial communitiesSurface-grown cellsPhage populationsSensitive phenotypeBacterial hostsOral microbiomePeptidoglycan structureWild typeMetagenomic analysisMutantsSpontaneous mutantsXH001Frameshift mutation
2021
BB0259 Encompasses a Peptidoglycan Lytic Enzyme Function for Proper Assembly of Periplasmic Flagella in Borrelia burgdorferi
Xu H, Hu B, Flesher DA, Liu J, Motaleb MA. BB0259 Encompasses a Peptidoglycan Lytic Enzyme Function for Proper Assembly of Periplasmic Flagella in Borrelia burgdorferi. Frontiers In Microbiology 2021, 12: 692707. PMID: 34659138, PMCID: PMC8517470, DOI: 10.3389/fmicb.2021.692707.Peer-Reviewed Original ResearchPeriplasmic flagellaFlagellar rodProper assemblyFlagellar hookCryo-electron tomographyPG sacculusProtein homologsΕ-ProteobacteriaPeptidoglycan sacculusLytic transglycosylaseΓ-ProteobacteriaDistinct proteinsFlgJOuter membraneFunctional flagellaFilament assemblyFunctional domainsEnzyme functionCell wallMutant strainLyme disease spirocheteFlagellaEnzyme activityAssemblySacculus