2019
Structural Basis for Target-Directed MicroRNA Degradation
Sheu-Gruttadauria J, Pawlica P, Klum SM, Wang S, Yario TA, Schirle Oakdale NT, Steitz JA, MacRae IJ. Structural Basis for Target-Directed MicroRNA Degradation. Molecular Cell 2019, 75: 1243-1255.e7. PMID: 31353209, PMCID: PMC6754277, DOI: 10.1016/j.molcel.2019.06.019.Peer-Reviewed Original ResearchConceptsTarget-directed miRNA degradationMiRNA 3' endMicroRNA degradationMiRNA degradationHuman Ago2MiRNA activityMiRNA stabilityStructural basisGene expressionTarget RNALinker flexibilityMiRNAsEnd displaysFlexible linkerRNAKey determinantArgonauteHAgo2Enzymatic attackAgo2DegradationDuplexMicroRNAsMiRNAIsoforms
2009
miRNPs: versatile regulators of gene expression in vertebrate cells1
Steitz JA, Vasudevan S. miRNPs: versatile regulators of gene expression in vertebrate cells1. Biochemical Society Transactions 2009, 37: 931-935. PMID: 19754429, DOI: 10.1042/bst0370931.Peer-Reviewed Original ResearchConceptsPost-transcriptional controlAssociation of Ago2Role of miRNAsImmature Xenopus oocytesTNFalpha AREProtein FXR1Contact-inhibited cellsTranslational regulationTranslation activationVersatile regulatorsTranslational efficiencyNegative regulatorGene expressionSpecific miRNACell cycleEffector moleculesCell growthXenopus oocytesAgo2FXR1Regulating the activity of microRNPs in vertebrate cells
Steitz J, Vasudevan S, Cazalla D. Regulating the activity of microRNPs in vertebrate cells. The FASEB Journal 2009, 23: 90.3-90.3. DOI: 10.1096/fasebj.23.1_supplement.90.3.Peer-Reviewed Original ResearchAU-rich sequencesPost-transcriptional controlAssociation of Ago2Role of microRNAsProtein FXR1Translation regulationContact-inhibited cellsVertebrate cellsTranslation upregulationTranslation activationTranslational efficiencyNegative regulatorCell cycleSpecific microRNAsQuiescent cellsS phaseH. saimiriEffector moleculesCell growthMicroRNPsXenopus oocytesMicroRNAsAgo2FXR1Monocyte differentiation