2019
How Complementary Targets Expose the microRNA 3′ End for Tailing and Trimming during Target-Directed microRNA Degradation
Pawlica P, Sheu-Gruttadauria J, MacRae IJ, Steitz JA. How Complementary Targets Expose the microRNA 3′ End for Tailing and Trimming during Target-Directed microRNA Degradation. Cold Spring Harbor Symposia On Quantitative Biology 2019, 84: 039321. PMID: 32019864, PMCID: PMC9161719, DOI: 10.1101/sqb.2019.84.039321.Peer-Reviewed Original ResearchTarget-directed miRNA degradationMiRNA 3' endCellular enzymesMiRNA seed sequencesMiRNA complementarityMiRNA decayArgonaute proteinsMicroRNA degradationMiRNA degradationMiRNA 5Posttranscriptional regulationMiRNA targetsCertain transcriptsSeed sequenceExtensive complementarityMolecular mechanismsMessenger RNATranscriptsEnzymeRegulationEnzymatic attackRecent advancesTargetMicroRNAsRNA
2014
3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells
Guo YE, Steitz JA. 3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells. RNA 2014, 20: 985-988. PMID: 24821854, PMCID: PMC4114695, DOI: 10.1261/rna.045054.114.Peer-Reviewed Original Research
2012
Association of Argonaute proteins and microRNAs can occur after cell lysis
Riley KJ, Yario TA, Steitz JA. Association of Argonaute proteins and microRNAs can occur after cell lysis. RNA 2012, 18: 1581-1585. PMID: 22836356, PMCID: PMC3425773, DOI: 10.1261/rna.034934.112.Peer-Reviewed Original ResearchConceptsHuman AgosMiRNA-protein complexesMicroRNA target identificationDirect mRNA targetsCore protein componentsArgonaute proteinsAGO proteinsMRNA targetsImmunoprecipitation experimentsProtein componentsMiRNA mimicsRNAEndogenous interactionCell lysisTarget identificationImmunopurification techniquesGlobal analysisProteinInteraction artifactsOrigin of interactionsExperimental approachArgonauteVivoAgoMiRNAs