2014
The Noncoding RNA Revolution—Trashing Old Rules to Forge New Ones
Cech TR, Steitz JA. The Noncoding RNA Revolution—Trashing Old Rules to Forge New Ones. Cell 2014, 157: 77-94. PMID: 24679528, DOI: 10.1016/j.cell.2014.03.008.Peer-Reviewed Original ResearchConceptsBiological functionsRNA-protein complexesLevel of transcriptionForeign nucleic acidsMost ncRNAsLong ncRNAsNcRNA researchRNA processingGenome rearrangementsNucleic acidsNoncoding RNAsGene expressionRNA structureNcRNAsBase pairingDNA synthesisRemarkable varietySnoRNPsRiboswitchGenomeSnRNPsRNAsRibosomesTranscriptionTelomerase
2010
Down-Regulation of a Host microRNA by a Viral Noncoding RNA
Cazalla D, Steitz JA. Down-Regulation of a Host microRNA by a Viral Noncoding RNA. Cold Spring Harbor Symposia On Quantitative Biology 2010, 75: 321-324. PMID: 21139068, PMCID: PMC5647998, DOI: 10.1101/sqb.2010.75.009.Peer-Reviewed Original ResearchConceptsHerpesvirus saimiriNoncoding RNAsHost cell gene expressionMiR-27Binding-dependent mannerAU-rich elementsViral noncoding RNAMarmoset T cellsMiRNA pathwayHost cell microRNAsViral life cycleConserved sequencesEctopic expressionMammalian virusesTarget genesTransient knockdownMutational analysisGene expressionHost microRNAsHSUR1Viral strategiesBase pairingDown regulationPrimate herpesvirusesLytic phase
1999
Guided tours: from precursor snoRNA to functional snoRNP
Weinstein L, Steitz J. Guided tours: from precursor snoRNA to functional snoRNP. Current Opinion In Cell Biology 1999, 11: 378-384. PMID: 10395551, DOI: 10.1016/s0955-0674(99)80053-2.Peer-Reviewed Original Research
1996
Length suppression in histone messenger RNA 3′-end maturation: Processing defects of insertion mutant premessenger RNAs can be compensated by insertions into the U7 small nuclear RNA
Scharl E, Steitz J. Length suppression in histone messenger RNA 3′-end maturation: Processing defects of insertion mutant premessenger RNAs can be compensated by insertions into the U7 small nuclear RNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 14659-14664. PMID: 8962110, PMCID: PMC26191, DOI: 10.1073/pnas.93.25.14659.Peer-Reviewed Original ResearchConceptsHistone downstream elementU7 RNAHistone messenger RNASmall nuclear RNARNA processing systemSmall ribonucleoproteinPremessenger RNANuclear RNAPre-mRNAU7 small nuclear RNADownstream elementsCleavage siteRNAMessenger RNAXenopus oocytesBase pairingProcessing defectsU7First demonstrationHistonesRNAsRibonucleoproteinInsertionMRNASites
1993
A base-pairing interaction between U2 and U6 small nuclear RNAs occurs in > 150S complexes in HeLa cell extracts: implications for the spliceosome assembly pathway.
Wassarman DA, Steitz JA. A base-pairing interaction between U2 and U6 small nuclear RNAs occurs in > 150S complexes in HeLa cell extracts: implications for the spliceosome assembly pathway. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 7139-7143. PMID: 8346227, PMCID: PMC47091, DOI: 10.1073/pnas.90.15.7139.Peer-Reviewed Original ResearchConceptsU6 small nuclear RNASmall nuclear RNAS100 extractsNuclear RNASer/Arg-rich (SR) proteinsBase pairingNuclear extractsLarge complexesArg-rich proteinsPre-RNA splicingSpliceosome assembly pathwayHeLa cell extractsHeLa nuclear extractsBase-pairing interactionsBase pair formsPossible functional relationshipRibonucleoprotein complexesSpliceosome complexMammalian cellsAssembly pathwaySplicing substratePsoralen crosslinkingGlycerol gradientsCell extractsSplicing
1987
Structural Analysis of the Human U3 Ribonucleoprotein Particle Reveal a Conserved Sequence Available for Base Pairing with Pre-rRna
Parker K, Steitz J. Structural Analysis of the Human U3 Ribonucleoprotein Particle Reveal a Conserved Sequence Available for Base Pairing with Pre-rRna. Molecular And Cellular Biology 1987, 7: 2899-2913. DOI: 10.1128/mcb.7.8.2899-2913.1987.Peer-Reviewed Original ResearchProtein-RNA interactionsSecondary structureSubsequent reverse transcriptionRNA secondary structureHuman U3Phosphorylated proteinsConserved sequencesProcessing eventsPre-rRNAAlternative functionsBase pairsNucleotides -159Specific nucleasesHeLa cellsProtein constituentsRNase ABase pairingRNPProteinNonphosphorylated proteinsRRNAReverse transcriptionSequenceKilodaltonsNucleotides 65
1979
Genetic Signals and Nucleotide Sequences in Messenger RNA
Steitz J. Genetic Signals and Nucleotide Sequences in Messenger RNA. Biological Regulation And Development 1979, 349-399. DOI: 10.1007/978-1-4684-3417-0_9.Peer-Reviewed Original ResearchRNA moleculesMessenger RNAMessenger RNA moleculesAminoacyl-tRNA synthetasesSequence of nucleotidesThree-dimensional foldingSequence of basesGenetic signalsRNA functionRibosomal RNANucleotide sequenceTRNA moleculesGenetic informationU residuesWatson-Crick base pairsTertiary structureBase pairsPolypeptide chainRNABase pairingSpecific recognitionSequenceProteinString of beadsSynthetases
1974
High resolution proton NMR study of an isolated fragment of R17 bacteriophage mRNA
HILBERS CW, SHULMAN RG, YAMANE T, STEITZ JA. High resolution proton NMR study of an isolated fragment of R17 bacteriophage mRNA. Nature 1974, 248: 225-226. PMID: 4819416, DOI: 10.1038/248225a0.Peer-Reviewed Original ResearchConceptsHelical regionBase pairsBase pairingWatson-Crick base pairingIsolated fragmentsSecondary structureNMR spectraModel systemNuclear magnetic resonance spectraHigh-resolution nuclear magnetic resonance spectraNucleic acidsHydrogen-bonded protonsMagnetic resonance spectraProton NMR studiesAdjacent basesLeaf modelFragmentsNH protonsNMR studiesTRNAResonance spectraHigh-resolution proton NMR studiesHelixPairingGuanine