1993
Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5′ splice sites in vivo.
Cortes JJ, Sontheimer EJ, Seiwert SD, Steitz JA. Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5′ splice sites in vivo. The EMBO Journal 1993, 12: 5181-5189. PMID: 8262061, PMCID: PMC413781, DOI: 10.1002/j.1460-2075.1993.tb06213.x.Peer-Reviewed Original ResearchConceptsSplice siteRabbit beta-globin geneBeta-globin transcriptsBeta-globin geneU5 genesConserved loopMRNA splicingU5 snRNASecond intronFirst exonExon sequencesLoop mutantsSecond exonExpression vectorTransient transfectionCryptic sitesNovel siteHeLa cellsGT dinucleotideExonsExon 1U5 sequencesSnRNAMutationsVivo system
1983
The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro
Mount S, Pettersson I, Hinterberger M, Karmas A, Steitz J. The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro. Cell 1983, 33: 509-518. PMID: 6190573, DOI: 10.1016/0092-8674(83)90432-4.Peer-Reviewed Original ResearchConceptsRNA-protein complexesSplice siteU1 snRNPGlobin RNAPartial proteinase K digestionU1 snRNPsMouse beta-globin geneBacteriophage T7 RNA polymeraseEucaryotic cell nucleusMessenger RNA splicingT7 RNA polymeraseRecombinant DNA clonesBeta-globin geneT1 ribonuclease digestionSplice site sequencesSmall intronsSnRNP proteinsRNA splicingSnRNP complexDNA clonesRNA polymeraseSnRNPSite sequenceRibonuclease digestionProteinase K digestion