2012
EBV and human microRNAs co‐target oncogenic and apoptotic viral and human genes during latency
Riley KJ, Rabinowitz GS, Yario TA, Luna JM, Darnell RB, Steitz JA. EBV and human microRNAs co‐target oncogenic and apoptotic viral and human genes during latency. The EMBO Journal 2012, 31: 2207-2221. PMID: 22473208, PMCID: PMC3343464, DOI: 10.1038/emboj.2012.63.Peer-Reviewed Original ResearchConceptsHuman microRNAsLatent membrane protein 1Viral miRNA functionHigh-throughput sequencingHuman miRNA targetsMiRNA-binding sitesMiRNA functionEBV BHRF1Human genesMiRNA targetsMRNA targetsCellular miRNAsMembrane protein 1MiRNA clusterHuman miRNAsGene expressionCell cycleReporter assaysDistinct binding sitesViral mRNAsMiRNAsLytic genesLytic switchProtein 1EBV latent membrane protein 1
2009
miRNPs: versatile regulators of gene expression in vertebrate cells1
Steitz JA, Vasudevan S. miRNPs: versatile regulators of gene expression in vertebrate cells1. Biochemical Society Transactions 2009, 37: 931-935. PMID: 19754429, DOI: 10.1042/bst0370931.Peer-Reviewed Original ResearchConceptsPost-transcriptional controlAssociation of Ago2Role of miRNAsImmature Xenopus oocytesTNFalpha AREProtein FXR1Contact-inhibited cellsTranslational regulationTranslation activationVersatile regulatorsTranslational efficiencyNegative regulatorGene expressionSpecific miRNACell cycleEffector moleculesCell growthXenopus oocytesAgo2FXR1Regulating the activity of microRNPs in vertebrate cells
Steitz J, Vasudevan S, Cazalla D. Regulating the activity of microRNPs in vertebrate cells. The FASEB Journal 2009, 23: 90.3-90.3. DOI: 10.1096/fasebj.23.1_supplement.90.3.Peer-Reviewed Original ResearchAU-rich sequencesPost-transcriptional controlAssociation of Ago2Role of microRNAsProtein FXR1Translation regulationContact-inhibited cellsVertebrate cellsTranslation upregulationTranslation activationTranslational efficiencyNegative regulatorCell cycleSpecific microRNAsQuiescent cellsS phaseH. saimiriEffector moleculesCell growthMicroRNPsXenopus oocytesMicroRNAsAgo2FXR1Monocyte differentiation
2007
Switching from Repression to Activation: MicroRNAs Can Up-Regulate Translation
Vasudevan S, Tong Y, Steitz JA. Switching from Repression to Activation: MicroRNAs Can Up-Regulate Translation. Science 2007, 318: 1931-1934. PMID: 18048652, DOI: 10.1126/science.1149460.Peer-Reviewed Original ResearchMeSH Keywords3' Untranslated RegionsArgonaute ProteinsBase PairingCell CycleCell LineCell ProliferationComputational BiologyEukaryotic Initiation Factor-2Gene Expression RegulationHeLa CellsHMGA2 ProteinHumansInterphaseMicroRNAsProtein BiosynthesisRibonucleoproteinsRNA, MessengerRNA-Binding ProteinsTransfectionTumor Necrosis Factor-alphaUp-RegulationConceptsAU-rich elementsCell cycle arrestCycle arrestUntranslated regionMental retardation-related protein 1MicroRNA target sitesMicroRNA let-7Messenger RNA (mRNA) 3' untranslated regionsRegulates TranslationTranslation regulationTarget mRNAsGene expressionCell cycleCommon functionProtein 1ArgonauteTarget siteActivation signalsRepressionTumor necrosis factor-alpha mRNAMRNARegulationActivationArrestMicroRNPs
1985
Transfer RNA is an essential component of the ubiquitin- and ATP-dependent proteolytic system.
Ciechanover A, Wolin SL, Steitz JA, Lodish HF. Transfer RNA is an essential component of the ubiquitin- and ATP-dependent proteolytic system. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 1341-1345. PMID: 2983338, PMCID: PMC397256, DOI: 10.1073/pnas.82.5.1341.Peer-Reviewed Original ResearchConceptsATP-dependent proteolytic systemProteolytic systemATP-dependent degradationATP-requiring reactionATP-dependent pathwayProteolytic activityProgression of cellsProtein substratesTransfer RNAGene activationMammalian cellsUncharged tRNAReticulocyte extractsProtein degradationTRNA fractionReticulocyte systemMouse cellsCell cycleUbiquitinEssential componentRNAGel electrophoresisTRNAConjugated proteinsAutoimmune sera