1998
Overexpression of HuR, a nuclear–cytoplasmic shuttling protein, increases the in vivo stability of ARE‐containing mRNAs
Fan X, Steitz J. Overexpression of HuR, a nuclear–cytoplasmic shuttling protein, increases the in vivo stability of ARE‐containing mRNAs. The EMBO Journal 1998, 17: 3448-3460. PMID: 9628880, PMCID: PMC1170681, DOI: 10.1093/emboj/17.12.3448.Peer-Reviewed Original ResearchConceptsAU-rich elementsOverexpression of HuRUntranslated regionRNA recognition motif 3Beta-globin reporter mRNAClass II AU-rich elementsAnti-HuR antibodyMouse L929 cellsMRNA decayELAV familyVivo decay ratesDeletion mutantsReporter mRNAL929 cellsMotif 3HuR functionMRNA stabilityCytoplasmic compartmentRNA sequencesRRMs 3HuR proteinVivo roleMessenger RNAHuRHuR antibody
1993
The Cellular RNA-Binding Protein EAP Recognizes a Conserved Stem-Loop in the Epstein-Barr Virus Small RNA EBER 1
Toczyski D, Steitz J. The Cellular RNA-Binding Protein EAP Recognizes a Conserved Stem-Loop in the Epstein-Barr Virus Small RNA EBER 1. Molecular And Cellular Biology 1993, 13: 703-710. DOI: 10.1128/mcb.13.1.703-710.1993.Peer-Reviewed Original ResearchSmall RNAsCellular RNA-binding proteinsConserved stem loopRNA-binding proteinDetailed mutational analysisStem-loop structureSequence-specific mannerRNase protection experimentsDouble-stranded regionsDeletion mutantsEBER-1Mutational analysisStem loopFusion proteinProtection experimentsViral proteinsRNAProteinEpstein-Barr virus small RNAsEpstein-Barr virusBindingMutantsHerpesvirus papioNucleotidesHairpinThe cellular RNA-binding protein EAP recognizes a conserved stem-loop in the Epstein-Barr virus small RNA EBER 1.
Toczyski DP, Steitz JA. The cellular RNA-binding protein EAP recognizes a conserved stem-loop in the Epstein-Barr virus small RNA EBER 1. Molecular And Cellular Biology 1993, 13: 703-710. PMID: 8380232, PMCID: PMC358948, DOI: 10.1128/mcb.13.1.703.Peer-Reviewed Original ResearchConceptsCellular RNA-binding proteinsRNA-binding proteinDetailed mutational analysisStem-loop structureSequence-specific mannerRNase protection experimentsEpstein‐Barr virus small RNA EBER‐1Double-stranded regionsSmall RNAsDeletion mutantsEBER-1Mutational analysisFusion proteinProtection experimentsViral proteinsProteinRNAEpstein-Barr virusBindingMutantsHerpesvirus papioNucleotidesHairpinAntibodiesCells