2019
Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells.
Vallery TK, Steitz JA. Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells. Journal Of Visualized Experiments 2019 PMID: 31524859, PMCID: PMC6750728, DOI: 10.3791/59697.Peer-Reviewed Original ResearchConceptsRNA FISHSarcoma-associated herpesvirusSpecific RNAViral replication compartmentsSpecific gene productsSitu hybridizationKaposi's sarcoma-associated herpesvirusMultiple cell typesReplication compartmentsGene productsViral genesHost cellsCell typesQuantitative fluorescenceNuclear factoriesFishCell morphologyHuman hostMechanistic insightsSpatiotemporal activityUninfected cellsBehavior of biomoleculesRNAProteinCells
1982
Nucleotide sequence of γδ resolvase gene and demonstration that its gene product acts as a repressor of transcription
Reed R, Shibuya G, Steitz J. Nucleotide sequence of γδ resolvase gene and demonstration that its gene product acts as a repressor of transcription. Nature 1982, 300: 381-383. PMID: 6292730, DOI: 10.1038/300381a0.Peer-Reviewed Original ResearchConceptsRepressor of transcriptionSite-specific recombination systemResolvase proteinAmino acid sequenceSite-specific recombinationElement-encoded proteinsIntercistronic regionGene initiatesFrequency of transpositionGene productsNucleotide sequenceAcid sequenceRecombination systemTranscription systemGenetic analysisΓδ resolvaseResolvaseGenesTranscriptionProteinResolvase geneRelated transposonsTransposaseModel substrateRecombination