1991
An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs.
Baserga SJ, Yang XD, Steitz JA. An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs. The EMBO Journal 1991, 10: 2645-2651. PMID: 1714385, PMCID: PMC452965, DOI: 10.1002/j.1460-2075.1991.tb07807.x.Peer-Reviewed Original ResearchConceptsBox CU3 snRNANucleolar small RNAsSite-specific mutationsShort nucleotide sequencesFibrillarin proteinSmall RNAsDeletion analysisCommon binding siteBox DNucleotide sequenceSnRNPsMajor familiesSnRNAU3 snRNPRNAInput RNAFibrillarinBinding sitesC sequencesBindingProteinSequenceAnti-fibrillarin autoantibodiesBiogenesis
1988
Trans splicing involves a novel form of small nuclear ribonucleoprotein particles
Bruzik J, Doren K, Hirsh D, Steitz J. Trans splicing involves a novel form of small nuclear ribonucleoprotein particles. Nature 1988, 335: 559-562. PMID: 2971142, DOI: 10.1038/335559a0.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinSL RNACis splicingSplice siteNuclear ribonucleoproteinPrecursor-messenger RNA (pre-mRNA) transcriptsHeLa cell nuclear extractsSame nuclear compartmentTrans-splicing reactionCell nuclear extractsPossible secondary structuresMessenger RNA transcriptsSm snRNPSplice acceptor siteTrans splicingCellular machineryLeader transcriptNuclear compartmentNucleotide sequenceSplicing processRNA transcriptsNuclear extractsSnRNPSplicingSecondary structure
1982
Nucleotide sequence of γδ resolvase gene and demonstration that its gene product acts as a repressor of transcription
Reed R, Shibuya G, Steitz J. Nucleotide sequence of γδ resolvase gene and demonstration that its gene product acts as a repressor of transcription. Nature 1982, 300: 381-383. PMID: 6292730, DOI: 10.1038/300381a0.Peer-Reviewed Original ResearchConceptsRepressor of transcriptionSite-specific recombination systemResolvase proteinAmino acid sequenceSite-specific recombinationElement-encoded proteinsIntercistronic regionGene initiatesFrequency of transpositionGene productsNucleotide sequenceAcid sequenceRecombination systemTranscription systemGenetic analysisΓδ resolvaseResolvaseGenesTranscriptionProteinResolvase geneRelated transposonsTransposaseModel substrateRecombination
1980
Are snRNPs involved in splicing?
Lerner M, Boyle J, Mount S, Wolin S, Steitz J. Are snRNPs involved in splicing? Nature 1980, 283: 220-224. PMID: 7350545, DOI: 10.1038/283220a0.Peer-Reviewed Original ResearchConceptsSmall nuclear RNA speciesProminent nuclear proteinsRNA-protein complexesSmall RNA moleculesSmall nuclear ribonucleoproteinNuclear RNA speciesSnRNA speciesSnRNAs U1RNA speciesU6 RNASnRNA moleculesNuclear proteinsNuclear ribonucleoproteinNucleotide sequenceU1 RNARNA moleculesSnRNPsExtensive complementaritySplice junctionsNuclear locationLines of evidenceAnti-Sm seraCell nucleiHnRNA moleculesMouse Ehrlich
1979
Genetic Signals and Nucleotide Sequences in Messenger RNA
Steitz J. Genetic Signals and Nucleotide Sequences in Messenger RNA. Biological Regulation And Development 1979, 349-399. DOI: 10.1007/978-1-4684-3417-0_9.Peer-Reviewed Original ResearchRNA moleculesMessenger RNAMessenger RNA moleculesAminoacyl-tRNA synthetasesSequence of nucleotidesThree-dimensional foldingSequence of basesGenetic signalsRNA functionRibosomal RNANucleotide sequenceTRNA moleculesGenetic informationU residuesWatson-Crick base pairsTertiary structureBase pairsPolypeptide chainRNABase pairingSpecific recognitionSequenceProteinString of beadsSynthetases
1977
The nucleotide sequence of a major glycine transfer RNA from the posterior silk gland of Bombyx mori L.
Zúñiga M, Steitz J. The nucleotide sequence of a major glycine transfer RNA from the posterior silk gland of Bombyx mori L. Nucleic Acids Research 1977, 4: 4175-4196. PMID: 414206, PMCID: PMC343233, DOI: 10.1093/nar/4.12.4175.Peer-Reviewed Original Research
1974
Nucleotide sequences of the 5′ and 3′ termini of bacteriophage T7 early messenger RNAs synthesized in vivo: Evidence for sequence specificity in RNA processing
Kramer R, Rosenberg M, Steitz J. Nucleotide sequences of the 5′ and 3′ termini of bacteriophage T7 early messenger RNAs synthesized in vivo: Evidence for sequence specificity in RNA processing. Journal Of Molecular Biology 1974, 89: 767-776. PMID: 4449132, DOI: 10.1016/0022-2836(74)90051-5.Peer-Reviewed Original ResearchConceptsEarly regionMessenger RNAAdenylic acid residuesEarly messenger RNATwo-dimensional electrophoretic techniquesLarger precursor moleculeMonocistronic messengerRNA processingGene 0.3RNA speciesNucleotide sequenceSequence specificityAcid residuesMessenger speciesSequence analysisInitiator RNASequence heterogeneityRNAEarly RNATerminus
1972
Oligonucleotide Sequence of Replicase Initiation Site in Qβ RNA
STEITZ J. Oligonucleotide Sequence of Replicase Initiation Site in Qβ RNA. Nature 1972, 236: 71-75. PMID: 4502455, DOI: 10.1038/newbio236071a0.Peer-Reviewed Original ResearchConceptsReplicase geneQβ RNAMajor coat proteinN-terminal sequenceCoat cistronTermination signalReplicase cistronNucleotide sequenceCoat proteinRNA genomeInitiator codonInitiation siteQβ replicaseGenesProteinBacteriophage QβCistronUGA suppressorRNAVirus particlesSequenceOligonucleotide sequencesProtein7GenomeReplicase