1997
A new strategy for introducing photoactivatable 4-thiouridine ((4S)U) into specific positions in a long RNA molecule.
Yu YT, Steitz JA. A new strategy for introducing photoactivatable 4-thiouridine ((4S)U) into specific positions in a long RNA molecule. RNA 1997, 3: 807-10. PMID: 9214662, PMCID: PMC1369526.Peer-Reviewed Original ResearchConceptsPre-mRNAPre-mRNA substrateAT-AC intronsPhage RNA polymeraseRNA-DNA chimerasFull-length RNALong RNA moleculesRNA polymeraseRNA moleculesT4 RNA ligaseT4 DNA ligaseRNA ligaseDNA ligaseRNARNase H cleavageLigaseSpecific sitesSpecific positionsIntronsPolymeraseChimerasNew strategyCleavageOligonucleotideH cleavage
1993
A small nucleolar RNA is processed from an intron of the human gene encoding ribosomal protein S3.
Tycowski KT, Shu MD, Steitz JA. A small nucleolar RNA is processed from an intron of the human gene encoding ribosomal protein S3. Genes & Development 1993, 7: 1176-1190. PMID: 8319909, DOI: 10.1101/gad.7.7a.1176.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCell NucleolusCell-Free SystemConserved SequenceElectrophoresis, Polyacrylamide GelHeLa CellsHumansIntronsMolecular Sequence DataNucleic Acid ConformationRestriction MappingRibosomal ProteinsRNA PrecursorsRNA Processing, Post-TranscriptionalRNA, Small NuclearSequence Analysis, RNAUracil NucleotidesConceptsSmall nucleolar RNAsNucleolar RNAsRibosomal protein S3 geneNuclear RNA polymerasesSingle-copy geneSingle primary transcriptRibosomal protein S3Secondary structure modelStem-loop structureVertebrate cellsNucleolar proteinsProtein S3Transcription signalsHuman genesRNA polymerasePrimary transcriptConserved sequencesS3 geneNucleolar snRNASame strandS3 mRNANucleotides downstreamMature endNucleolar componentsIntron 1
1983
The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro
Mount S, Pettersson I, Hinterberger M, Karmas A, Steitz J. The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro. Cell 1983, 33: 509-518. PMID: 6190573, DOI: 10.1016/0092-8674(83)90432-4.Peer-Reviewed Original ResearchConceptsRNA-protein complexesSplice siteU1 snRNPGlobin RNAPartial proteinase K digestionU1 snRNPsMouse beta-globin geneBacteriophage T7 RNA polymeraseEucaryotic cell nucleusMessenger RNA splicingT7 RNA polymeraseRecombinant DNA clonesBeta-globin geneT1 ribonuclease digestionSplice site sequencesSmall intronsSnRNP proteinsRNA splicingSnRNP complexDNA clonesRNA polymeraseSnRNPSite sequenceRibonuclease digestionProteinase K digestion
1974
T7 Early messenger RNAs are the direct products of ribonuclease III cleavage
Rosenberg M, Kramer R, Steitz J. T7 Early messenger RNAs are the direct products of ribonuclease III cleavage. Journal Of Molecular Biology 1974, 89: 777-782. PMID: 4615167, DOI: 10.1016/0022-2836(74)90052-7.Peer-Reviewed Original Research