1995
Modulation of 5' splice site choice in pre-messenger RNA by two distinct steps.
Tarn WY, Steitz JA. Modulation of 5' splice site choice in pre-messenger RNA by two distinct steps. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 2504-2508. PMID: 7708674, PMCID: PMC42246, DOI: 10.1073/pnas.92.7.2504.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinU1 small nuclear ribonucleoproteinSplice site choicePre-messenger RNASR proteinsSplice site selectionSplice siteSite choiceSer/Arg-rich (SR) proteinsU2 small nuclear ribonucleoproteinU1 snRNP functionsHeLa cell nuclear extractsEssential splicing factorArg-rich proteinsCell nuclear extractsDistinct stepsSnRNP functionSite selectionSplicing factorsU1 bindingNuclear ribonucleoproteinU1 RNANuclear extractsProteinRNA
1993
A base-pairing interaction between U2 and U6 small nuclear RNAs occurs in > 150S complexes in HeLa cell extracts: implications for the spliceosome assembly pathway.
Wassarman DA, Steitz JA. A base-pairing interaction between U2 and U6 small nuclear RNAs occurs in > 150S complexes in HeLa cell extracts: implications for the spliceosome assembly pathway. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 7139-7143. PMID: 8346227, PMCID: PMC47091, DOI: 10.1073/pnas.90.15.7139.Peer-Reviewed Original ResearchConceptsU6 small nuclear RNASmall nuclear RNAS100 extractsNuclear RNASer/Arg-rich (SR) proteinsBase pairingNuclear extractsLarge complexesArg-rich proteinsPre-RNA splicingSpliceosome assembly pathwayHeLa cell extractsHeLa nuclear extractsBase-pairing interactionsBase pair formsPossible functional relationshipRibonucleoprotein complexesSpliceosome complexMammalian cellsAssembly pathwaySplicing substratePsoralen crosslinkingGlycerol gradientsCell extractsSplicing